New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =17 (0.220)39 (0.570) 34/220 NT 74.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 12 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related

TTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAGGA  >  NC_000913/1209471‑1209762
                                                                                                                                                    |                                                                                                                                               
ccctatgcagatgcttttgcggcattccacccccactgaatgattactgattggtcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaac                                                                                                                                                 <  3:91626‑M2/1‑1 (MQ=255)
     ggcagatgcttttgcggcattccacccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTa                                                                                                                                            <  6:293078‑M2/6‑1 (MQ=255)
      gTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTa                                                                                                                                            <  2:205748/148‑1 (MQ=255)
        agatgcttttgcggcattccacccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATg                                                                                                                                          >  2:36313‑M2/141‑148 (MQ=255)
           tgcttttgcggcattccacccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGaaaa                                                                                                                                      <  1:36313‑M2/12‑1 (MQ=255)
               gCCTTTGCCAATTCCGGGTATTATTCAGAAGAAAAAGCGGCACCATTGCATTTAAGCCAGCCTGTTGGCGGAGTGGATGAAGGACACGGAACCGGTACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCATCCTTTATGAAAATg                                                                                                                                    >  4:195738/1‑147 (MQ=255)
                 tgcggcattccacccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccagtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATgaag                                                                                                                                 <  1:60948‑M2/17‑1 (MQ=255)
                    tGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATgaagaa                                                                                                                               <  7:258146/147‑1 (MQ=255)
                           cacccccactgaatgattactgattgctcactaccaccaatcataaccggtatagctatgaaaccgttgcgtcctaatgttgccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAacatg                                                                                                                       <  4:346792‑M2/27‑3 (MQ=255)
                              ccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATg                                                                                                                                          >  1:159416‑M2/119‑126 (MQ=255)
                              ccccactgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATg                                                                                                                                          <  2:159416‑M2/8‑1 (MQ=255)
                               ggTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGATCCGGGACACCAACAGGTAATGCAGAGACTTCTCCAAACCCAACTTTTATGAAAATGAAGAAATAACAAGCAAAt                                                                                                                  >  5:36532/1‑149 (MQ=255)
                                    tgaatgattactgattgctcaataccaccaatcataacgggtatagctatgaaaccggtgcgtcctaaagtagcccttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAATTGAAGAAATAACAAGCAAATGGcat                                                                                                             <  1:251116‑M2/37‑1 (MQ=255)
                                    tgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACaa                                                                                                                        <  7:314189‑M2/26‑1 (MQ=255)
                                    tgaatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACaa                                                                                                                        >  8:314189‑M2/113‑138 (MQ=255)
                                      ttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACACGCAAATGGcatca                                                                                                           >  7:283286/1‑149 (MQ=255)
                                      aatgattactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGcatca                                                                                                           >  4:3493‑M2/111‑149 (MQ=255)
                                          attactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctcattctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATcattgc                                                                                                       >  3:249721‑M2/107‑147 (MQ=255)
                                           ttactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGttt                                                                                                                                             >  7:86310‑M2/106‑110 (MQ=255)
                                           ttactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGttt                                                                                                                                             <  8:86310‑M2/5‑1 (MQ=255)
                                           ttactgattgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCt                                                                                                      >  8:335898‑M2/106‑149 (MQ=255)
                                                 attgctcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCtttt                                                                                                <  2:368834‑M2/50‑1 (MQ=255)
                                                      tcaataccaccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAg                                                                                           >  6:66681‑M2/95‑149 (MQ=255)
                                                             caccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGcatca                                                                                                           >  3:281523‑M2/88‑126 (MQ=255)
                                                             caccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGcatca                                                                                                           <  4:281523‑M2/39‑1 (MQ=255)
                                                             caccaatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGAtt                                                                                    >  1:60715‑M2/88‑149 (MQ=255)
                                                                caatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGc                                                                                                    <  2:6541‑M2/46‑1 (MQ=255)
                                                                caatcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGc                                                                                                    >  1:6541‑M2/85‑130 (MQ=255)
                                                                aTTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTaa                                                                                 >  7:33065/1‑149 (MQ=255)
                                                                   tcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACAt                                                                              >  8:80401‑M2/82‑149 (MQ=255)
                                                                   tcataaccggtatagctatgaaaccggtgcgtcctaatgtagccgatgctgaattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACAt                                                                              >  5:34935‑M2/82‑149 (MQ=255)
                                                                      taaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGc                                                                                                    >  5:128655‑M2/79‑124 (MQ=255)
                                                                      taaccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGc                                                                                                    <  6:128655‑M2/46‑1 (MQ=255)
                                                                         ccggtatagctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTAt                                                                        >  6:35833‑M2/76‑149 (MQ=255)
                                                                               ttGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGc                                                                    <  2:146264/147‑1 (MQ=255)
                                                                               ttGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGAAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCt                                                                   <  2:196511/148‑1 (MQ=255)
                                                                                 ggCGGAGTGGCTGAAGGCCACGTAACCGGGACAACAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTAt                                                                 <  6:36532/148‑1 (MQ=255)
                                                                                 gctatgaaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTAt                                                                 <  2:60715‑M2/81‑1 (MQ=255)
                                                                                       aaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTAcgc                                                           <  5:66681‑M2/87‑1 (MQ=255)
                                                                                       aaaccggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTAcgc                                                           <  7:80401‑M2/87‑1 (MQ=255)
                                                                                            ggtgcgtcctaatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATc                                                      >  4:329407‑M2/57‑148 (MQ=255)
                                                                                                      aatgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGAc                                            >  5:97925‑M2/47‑148 (MQ=255)
                                                                                                       atgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGAc                                            <  7:335898‑M2/102‑1 (MQ=255)
                                                                                                       atgtagccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCa                                          <  6:97925‑M2/104‑1 (MQ=255)
                                                                                                        aaCCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAggg                                                                                         >  6:315109/1‑101 (MQ=255)
                                                                                                        aaCCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAggg                                                                                         <  5:315109/101‑1 (MQ=255)
                                                                                                            gccgttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAaca                                     <  3:3493‑M2/109‑1 (MQ=255)
                                                                                                               gttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAg                                  <  5:35833‑M2/112‑1 (MQ=255)
                                                                                                               gttgctgcattgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCCTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAaca                                     >  8:216367‑M2/38‑146 (MQ=255)
                                                                                                                    aaCAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTa                             >  1:115746/1‑149 (MQ=255)
                                                                                                                       attgagttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACaa                          >  5:149528‑M2/30‑149 (MQ=255)
                                                                                                                           agttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGt                       >  8:363911‑M2/26‑148 (MQ=255)
                                                                                                                             ttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGGTTTTACCAGGGGGAtt                                                                                    >  4:41095‑M2/24‑85 (MQ=255)
                                                                                                                             ttttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGAtt                                                                                    <  3:41095‑M2/62‑1 (MQ=255)
                                                                                                                               ttgctccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATgc                  >  7:75857‑M2/22‑149 (MQ=255)
                                                                                                                                   tccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTg                                                                      <  2:248070‑M2/76‑1 (MQ=255)
                                                                                                                                   tccttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTg                                                                      >  1:248070‑M2/18‑93 (MQ=255)
                                                                                                                                     cttctcccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTAACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGaa            <  2:111581‑M2/134‑1 (MQ=255)
                                                                                                                                           ccaaacccaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCGTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACtgtg       >  3:96021‑M2/10‑148 (MQ=255)
                                                                                                                                           ccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACtgtg       <  6:34935‑M2/139‑1 (MQ=255)
                                                                                                                                           ccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACtgtg       >  8:382320‑M2/10‑148 (MQ=255)
                                                                                                                                           ccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACtgtg       >  6:152163‑M2/10‑148 (MQ=255)
                                                                                                                                           ccaaaccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACtgtg       >  3:175899‑M2/10‑148 (MQ=255)
                                                                                                                                             aacccaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAg    <  8:283286‑M2/142‑1 (MQ=255)
                                                                                                                                               accaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAGGa  >  1:381550‑M2/6‑149 (MQ=255)
                                                                                                                                               accaaCGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAGGa  <  8:75857‑M2/144‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTGCAGGA  >  NC_000913/1209471‑1209762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.