Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,003,997	T→A	6.1%	A42A (GCT→GCA)	fliD →	flagellar filament capping protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,997	0	T	A	6.1%	214.5 / 3.7	66	A42A (GCT→GCA)	fliD	flagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base T (33/29);  new base A (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCAT  >  NC_000913/2003853‑2004143                                                                                                                                                 |                                                                                                                                                   aaaGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAAc                                                                                                                                                >  6:156080/1‑149 (MQ=255) aaaGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGGCTGGTTTTAAGTACCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTaaca                                                                                                                                                >  5:100421/1‑147 (MQ=255)  aaGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACt                                                                                                                                               <  7:322379/149‑1 (MQ=255)       aaaaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgcg                                                                                                                                          >  8:206606/1‑149 (MQ=255)       aaaaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgcg                                                                                                                                          >  8:168455/1‑149 (MQ=255)        aaaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgcgc                                                                                                                                         >  3:237396/1‑149 (MQ=255)        aaaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgcgc                                                                                                                                         >  2:268148/1‑149 (MQ=255)         aaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgc                                                                                                                                           >  3:162150/1‑146 (MQ=255)         aaGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAgc                                                                                                                                           <  4:162150/146‑1 (MQ=255)          aGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGcc                                                                                                                                        >  5:270218/1‑148 (MQ=255)           ggAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTa                                                                                                                                      <  5:77945/149‑1 (MQ=255)             aaaTCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACg                                                                                                                                    >  3:137587/1‑149 (MQ=255)                  gCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTAc                                                                                                                                <  4:237396/148‑1 (MQ=255)                    aTGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCt                                                                                                                             <  6:270218/149‑1 (MQ=255)                      ggCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTg                                                                                                                            >  2:54532/1‑148 (MQ=255)                         aaGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGaa                                                                                                                          >  5:372274/1‑147 (MQ=255)                           gTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAgc                                                                                                                      <  5:313047/149‑1 (MQ=255)                              tttCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAgcgc                                                                                                                    <  6:100421/148‑1 (MQ=255)                               ttCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAgcgcgc                                                                                                                  >  5:75633/1‑149 (MQ=255)                                tCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAgcgcgc                                                                                                                  <  6:372274/148‑1 (MQ=255)                                   tCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTgac                                                                                                              >  1:388064/1‑149 (MQ=255)                                       tGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACt                                                                                                          >  8:88040/1‑149 (MQ=255)                                         ggAGTCTGGTCAGGTCTGGATGTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACtt                                                                                                         <  3:340765/148‑1 (MQ=255)                                         ggAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACtt                                                                                                         <  7:88040/148‑1 (MQ=255)                                         ggAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACt                                                                                                          <  4:137587/147‑1 (MQ=255)                                          gAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTc                                                                                                       <  7:168455/149‑1 (MQ=255)                                               gggTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTa                                                                                                                                      <  1:23510/113‑1 (MQ=255)                                               gggTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTa                                                                                                                                      >  2:23510/1‑113 (MQ=255)                                                     ggTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCat                                                                                            <  2:388064/149‑2 (MQ=255)                                                           gATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTg                                                                                       >  1:45730/1‑148 (MQ=255)                                                             tttAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTAAGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCAt                                                                                    >  5:29045/1‑149 (MQ=255)                                                             tttAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACATAGCGCCTACGGTACTCTGAAAATCGCGCTGACGACTTTCCAGACCGCAAATACTCCAt                                                                                    >  4:243045/1‑149 (MQ=255)                                                                aaGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCaa                                                                                            >  6:357373/1‑138 (MQ=255)                                                                aaGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCaa                                                                                            <  5:357373/138‑1 (MQ=255)                                                                      cATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAg                                                                                                    <  7:332635/124‑1 (MQ=255)                                                                      cATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAg                                                                                                    <  7:337915/124‑1 (MQ=255)                                                                      cATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAg                                                                                                    >  8:332635/1‑124 (MQ=255)                                                                      cATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAg                                                                                                    >  8:337915/1‑124 (MQ=255)                                                                      cATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTaaa                                                                            <  1:54532/148‑1 (MQ=255)                                                                       aTCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGc                                                                          <  7:206606/149‑1 (MQ=255)                                                                         ccTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCAAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGcc                                                                         >  4:119242/1‑148 (MQ=255)                                                                         ccTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCAAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGcc                                                                         >  4:124321/1‑148 (MQ=255)                                                                             gATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCt                                                                    >  1:378482/1‑149 (MQ=255)                                                                                 gCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCttttt                                                                <  2:45730/149‑1 (MQ=255)                                                                                 gCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCtttt                                                                 <  5:156080/148‑1 (MQ=255)                                                                                    tCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCaa                                                                                            <  5:322853/118‑1 (MQ=255)                                                                                    tCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCaa                                                                                            >  6:322853/1‑118 (MQ=255)                                                                                      aCCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGc                                                           >  2:353487/1‑149 (MQ=255)                                                                                      aCCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTAACGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTcc                                                             <  5:61042/147‑1 (MQ=255)                                                                                           cgcgCAAAAAGCGACGCTAACCCCCATTTCATATCAGCATTCGTCTTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGccacca                                                      <  2:378482/149‑1 (MQ=255)                                                                                              gcAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCAAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGccaccagca                                                   <  3:119242/149‑1 (MQ=255)                                                                                              gcAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCAAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGccaccagca                                                   <  3:124321/149‑1 (MQ=255)                                                                                                aaaaaGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGc                                                                                               <  1:49442/103‑1 (MQ=255)                                                                                                aaaaaGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGc                                                                                               >  2:49442/1‑103 (MQ=255)                                                                                                                ccccATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCg                                                            >  2:121130/1‑122 (MQ=255)                                                                                                                ccccATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCg                                                            <  1:121130/122‑1 (MQ=255)                                                                                                                 cccATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACcgc                                >  8:94929/1‑149 (MQ=255)                                                                                                                         aaaTCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGAGCGCCATTACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGt                         <  6:75633/148‑1 (MQ=255)                                                                                                                              aGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGccac                    <  1:268148/148‑1 (MQ=255)                                                                                                                                      tcgtTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCggg           >  7:28980/1‑149 (MQ=255)                                                                                                                                            aCCGCTAAACTTAGCGCCTACGGTACGCTGAAACGCGCGCTGACGACTTTCCCGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCGCCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTacgc      >  5:101255/1‑145 (MQ=255)                                                                                                                                            aCCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGc     >  7:217544/1‑149 (MQ=255)                                                                                                                                            aCCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGc     >  1:79027/1‑149 (MQ=255)                                                                                                                                               gCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCAt  >  5:72481/1‑149 (MQ=255)                                                                                                                                                cTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGAt                                                                      <  2:67411/80‑1 (MQ=255)                                                                                                                                                cTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGAt                                                                      >  1:67411/1‑80 (MQ=255)                                                                                                                                                 |                                                                                                                                                   AAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAATCGTCGTTTACCGCTAAACTTAGCGCCTACGGTACGCTGAAAAGCGCGCTGACGACTTTCCAGACCGCCAATACTGCATTGTCTAAAGCCGATCTTTTTTCCGCCACCAGCACCACCAGCAGCACCACCGCGTTCAGTGCCACCACTGCGGGTAACGCCAT  >  NC_000913/2003853‑2004143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑