Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,101,165 G→T 5.9% S35Y (TCC→TAC)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,101,1650GT 5.9% 277.0 / 2.7 80S35Y (TCC→TAC) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base G (39/36);  new base T (3/2);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTCG  >  NC_000913/2101021‑2101309
                                                                                                                                                |                                                                                                                                                
gcagcaTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACg                                                                                                                                              >  3:335999/1‑149 (MQ=255)
 cagcaTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCGATCACTTCTTCCGTCTTCTCACGGGAACgg                                                                                                                                             >  7:70802/1‑149 (MQ=255)
 cagcaTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACgg                                                                                                                                             >  8:168732/1‑149 (MQ=255)
   gcaTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCATTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGtt                                                                                                                                           >  4:51974/1‑149 (MQ=255)
        cGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGaaa                                                                                                                                       <  1:128143/148‑1 (MQ=255)
          tGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATa                                                                                                                                    <  4:153515/149‑1 (MQ=255)
            ccTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATaga                                                                                                                                  <  7:168732/149‑1 (MQ=255)
                cACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGTAAATAGAGAc                                                                                                                               <  7:379470/148‑1 (MQ=255)
                  ccTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACgg                                                                                                                             >  5:45941/1‑148 (MQ=255)
                   cTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGta                                                                                                                           >  7:126486/1‑149 (MQ=255)
                   cTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAAACAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGACACGGta                                                                                                                           >  6:372969/1‑149 (MQ=255)
                      cTTTCACCATTACCAGGATGCGACGAGGCGTTTCCAGCGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTGCTTGCCTGGATTTTCGGCAATCACTTCTTCCGGCTTCTCACGGGAACGGTTGAAAATAGAGACGGtat                                                                                                                          <  2:72178/147‑1 (MQ=255)
                         tCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCa                                                                                                                     >  2:238667/1‑149 (MQ=255)
                           aCCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCGCCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCAc                                                                                                                    <  6:61062/148‑1 (MQ=255)
                             cATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGc                                                                                                                 >  1:31003/1‑149 (MQ=255)
                             cATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGc                                                                                                                 <  8:126486/149‑1 (MQ=255)
                               ttAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCtt                                                                                                               >  1:327708/1‑149 (MQ=255)
                                 aaCAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCttt                                                                                                              <  6:45941/148‑1 (MQ=255)
                                  aCAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTCTAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACTGTATAACCACGGCTTTc                                                                                                             <  3:301294/148‑1 (MQ=255)
                                    aGGATGCGACGATTCGTTTCCAGAGATTCGACAACCTCATTCACGGAATAGTAAGGAACCAGTTTCATGCCTGGATATTCGGCCTTCCCTTCTTCCGTCTTCTCACGGTAACGCGTGAAAATACAGACGGTTTACCCACGGCtttag                                                                                                            >  3:56778/1‑145 (MQ=255)
                                      gATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGt                                                                                                        >  3:23258/1‑149 (MQ=255)
                                          cgacgaGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGa                                                                                                     <  2:31003/148‑1 (MQ=255)
                                          cgacgaGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAg                                                                                                    <  8:52893/149‑1 (MQ=255)
                                              gaGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAgcgc                                                                                                 >  4:251642/1‑148 (MQ=255)
                                                   gTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGtt                                                                                           <  1:238667/149‑1 (MQ=255)
                                                      tCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGc                                                                                         <  6:250745/148‑1 (MQ=255)
                                                       ccAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGt                                                                                       >  2:247419/1‑149 (MQ=255)
                                                        cAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTc                                                                                      >  7:51601/1‑149 (MQ=255)
                                                          gagaTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATCACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTcc                                                                                     >  1:52888/1‑148 (MQ=255)
                                                            gaTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCAt                                                                                  <  8:51601/149‑1 (MQ=255)
                                                                 gACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTg                                                                             <  3:51974/149‑1 (MQ=255)
                                                                  aCAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGTAACGGTTGAAAATAGAGACGGTATAACCACGGCtt                                                                                                               <  4:51180/114‑1 (MQ=255)
                                                                  aCAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGTAACGGTTGAAAATAGAGACGGTATAACCACGGCtt                                                                                                               >  3:51180/1‑114 (MQ=255)
                                                                  aCAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGTAACGGTTGAAAATAGAGACGGTATAACCACGGCtt                                                                                                               <  4:46427/114‑1 (MQ=255)
                                                                  aCAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGTAACGGTTGAAAATAGAGACGGTATAACCACGGCtt                                                                                                               >  3:46427/1‑114 (MQ=255)
                                                                    aaaCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCa                                                                          >  7:174746/1‑149 (MQ=255)
                                                                    aaaCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCa                                                                          >  8:212179/1‑149 (MQ=255)
                                                                        tctTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGc                                                                                         <  7:362399/130‑1 (MQ=255)
                                                                        tctTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGc                                                                                         >  8:362399/1‑130 (MQ=255)
                                                                         ctTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATAcc                                                                      >  8:172309/1‑148 (MQ=255)
                                                                          tttCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACg                                                                                                                              <  4:233980/91‑1 (MQ=255)
                                                                          tttCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGa                                                                    >  4:175153/1‑149 (MQ=255)
                                                                          tttCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTATTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCACATCACTGCCATACCGa                                                                    >  5:292758/1‑149 (MQ=255)
                                                                               ccGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGaaaa                                                                                                                                      <  1:198859/78‑1 (MQ=255)
                                                                               ccGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGaaaa                                                                                                                                      >  2:198859/1‑78 (MQ=255)
                                                                                   ataGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                           >  2:247525/1‑149 (MQ=255)
                                                                                    taGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACTGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGAt                                                          <  5:372969/149‑1 (MQ=255)
                                                                                    taGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                           <  6:292758/148‑1 (MQ=255)
                                                                                    taGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                           <  3:251642/148‑1 (MQ=255)
                                                                                    taGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGAt                                                          <  4:335999/149‑1 (MQ=255)
                                                                                      gTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCt                                                        >  6:175038/1‑149 (MQ=255)
                                                                                       tAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGc                                                                                         >  8:120772/1‑115 (MQ=255)
                                                                                       tAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGc                                                                                         <  7:120772/115‑1 (MQ=255)
                                                                                       tAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATAc                                                                       >  8:52217/1‑133 (MQ=255)
                                                                                       tAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATAc                                                                       <  7:52217/133‑1 (MQ=255)
                                                                                         aGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGt                                                      <  1:247419/148‑1 (MQ=255)
                                                                                            aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCt                                                  >  5:258609/1‑149 (MQ=255)
                                                                                            aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCt                                                  >  3:245291/1‑149 (MQ=255)
                                                                                              ccAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTg                                                <  7:172309/149‑1 (MQ=255)
                                                                                               cAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTg                                                >  8:193213/1‑148 (MQ=255)
                                                                                                 gTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGc                                                   >  8:232062/1‑143 (MQ=255)
                                                                                                 gTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGc                                                   <  7:232062/143‑1 (MQ=255)
                                                                                                        gCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGCCGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACa                                       >  6:278988/1‑148 (MQ=255)
                                                                                                        gCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACa                                       <  7:193213/148‑1 (MQ=255)
                                                                                                         ccTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCAAGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACAt                                      <  4:245291/148‑1 (MQ=255)
                                                                                                          cTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACa                                       >  4:163673/1‑146 (MQ=255)
                                                                                                          cTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACa                                       <  3:163673/146‑1 (MQ=255)
                                                                                                            ggATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATa                                     <  6:178385/146‑1 (MQ=255)
                                                                                                            ggATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATa                                     >  5:178385/1‑146 (MQ=255)
                                                                                                                 ttCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGt                              <  5:175038/148‑1 (MQ=255)
                                                                                                                     gCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGgt                         <  8:174746/149‑1 (MQ=255)
                                                                                                                      cAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGgtg                        >  5:11298/1‑149 (MQ=255)
                                                                                                                       aaTCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGCGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGAGTCCCATCACTGCCATAACGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCACgtgt                       >  3:91708/1‑149 (MQ=255)
                                                                                                                           aCTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGAt                    <  6:11298/148‑1 (MQ=255)
                                                                                                                             ttcttcCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTACAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCAc                 >  5:269620/1‑149 (MQ=255)
                                                                                                                                     tcttctCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTg          >  6:7852/1‑148 (MQ=255)
                                                                                                                                         ctcACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGaa        <  3:175153/146‑1 (MQ=255)
                                                                                                                                            aCGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCtt                                                 >  7:73124/1‑102 (MQ=255)
                                                                                                                                            aCGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCtt                                                 <  8:73124/102‑1 (MQ=255)
                                                                                                                                            aCGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTcg  >  4:324592/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTCG  >  NC_000913/2101021‑2101309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑