Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,581 C→T 5.1% intergenic (‑61/+21) ogrK ← / ← yegZ orphan Ogr protein, positive regulator of P2 growth/pseudogene, gpD phage P2‑like protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,5810CT 5.1% 256.0 / 2.4 78intergenic (‑61/+21)ogrK/yegZorphan Ogr protein, positive regulator of P2 growth/pseudogene, gpD phage P2‑like protein D
Reads supporting (aligned to +/- strand):  ref base C (38/36);  new base T (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

TGGTGATAACGCTCTTTTGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGCTCGTCTAT  >  NC_000913/2167435‑2167727
                                                                                                                                                  |                                                                                                                                                  
tGGTGATAACGCTCTTTTGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTacaaaaca                                                                                                                                                   >  7:347951/1‑148 (MQ=255)
          gCTCTTTTGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGataaaa                                                                                                                                        >  1:246387/1‑149 (MQ=255)
          gCTCTTTTGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGataaaa                                                                                                                                        >  6:297156/1‑149 (MQ=255)
              ttttGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGataaaataa                                                                                                                                     >  2:191206/1‑148 (MQ=255)
                ttGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGAtaaaataaaa                                                                                                                                   <  4:71460/148‑1 (MQ=255)
                  gTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAAc                                                                                                                                 <  8:347951/148‑1 (MQ=255)
                      tgtCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCAc                                                                                                                            <  1:42579/149‑1 (MQ=255)
                           gTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTACGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCAt                                                                                                                        >  4:254462/1‑148 (MQ=255)
                               tataGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTAtc                                                                                                                    <  5:337992/148‑1 (MQ=255)
                                 taGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTAtcttc                                                                                                                 <  2:246387/149‑1 (MQ=255)
                                   gCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTg                                                                                                               >  6:88226/1‑149 (MQ=255)
                                      gCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGttt                                                                                                            >  8:112409/1‑149 (MQ=255)
                                           tACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTa                                                                                                        <  1:191206/148‑1 (MQ=255)
                                             cgcgCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTAccc                                                                                                     <  5:122485/149‑1 (MQ=255)
                                                      gCGGCATGCCGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAg                                                                                             >  4:237888/1‑148 (MQ=255)
                                                              cTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCTTTTTTGTTTATTGTGCCATTTTATTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTg                                                                                     <  1:347791/148‑1 (MQ=255)
                                                                 gCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGc                                                                                        <  5:236829/142‑1 (MQ=255)
                                                                 gCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCt                                                                                 >  7:220775/1‑149 (MQ=255)
                                                                 gCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATAAGAAAGc                                                                                        >  6:236829/1‑142 (MQ=255)
                                                                   aTAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTc                                                                                >  7:372839/1‑148 (MQ=255)
                                                                   aTAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTc                                                                                >  2:44739/1‑148 (MQ=255)
                                                                   aTAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTc                                                                                >  8:351360/1‑148 (MQ=255)
                                                                      aaGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAg                                                                             <  7:351360/148‑1 (MQ=255)
                                                                            aaTGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAAt                                                                      <  8:372839/149‑1 (MQ=255)
                                                                            aaTGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAAt                                                                      >  8:368160/1‑149 (MQ=255)
                                                                              tGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAg                                                                                         >  8:147219/1‑128 (MQ=255)
                                                                              tGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAg                                                                                         <  7:147219/128‑1 (MQ=255)
                                                                              tGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGc                                                                                        <  7:280900/129‑1 (MQ=255)
                                                                              tGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGc                                                                                        >  8:280900/1‑129 (MQ=255)
                                                                               gAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAAc                                                                                                                                 <  3:310359/87‑1 (MQ=255)
                                                                               gAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAAc                                                                                                                                 >  4:310359/1‑87 (MQ=255)
                                                                                aaaCATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTAtcttct                                                                                                                <  1:142157/103‑1 (MQ=255)
                                                                                aaaCATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTAtcttct                                                                                                                >  2:142157/1‑103 (MQ=255)
                                                                                  aCATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGaaa                                                                                          <  1:27200/123‑1 (MQ=255)
                                                                                  aCATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGaaa                                                                                          >  2:27200/1‑123 (MQ=255)
                                                                                  aCATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGAcgtc                                                                <  1:44739/149‑1 (MQ=255)
                                                                                    aTCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTaa                                                                       >  3:232734/1‑140 (MQ=255)
                                                                                    aTCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTaa                                                                       <  4:232734/140‑1 (MQ=255)
                                                                                    aTCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGAcgtcgt                                                              <  4:128593/149‑1 (MQ=255)
                                                                                     tCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTaa                                                                       <  7:73841/139‑1 (MQ=255)
                                                                                     tCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTaa                                                                       >  8:73841/1‑139 (MQ=255)
                                                                                               ctctTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCtat                                                   >  2:388016/1‑149 (MQ=255)
                                                                                               ctctTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACACCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCtat                                                   >  4:224927/1‑149 (MQ=255)
                                                                                                tctTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCtat                                                   >  5:179657/1‑148 (MQ=255)
                                                                                                     cGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCta                                                    >  1:48886/1‑142 (MQ=255)
                                                                                                     cGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCta                                                    <  2:48886/142‑1 (MQ=255)
                                                                                                     cGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCa                                             <  5:88226/149‑1 (MQ=255)
                                                                                                            gttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAtg                                      >  8:260425/1‑149 (MQ=255)
                                                                                                            gttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAt                                       <  5:297156/148‑1 (MQ=255)
                                                                                                             ttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTc                                                                                                           <  5:16889/79‑1 (MQ=255)
                                                                                                             ttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTc                                                                                                           >  6:16889/1‑79 (MQ=255)
                                                                                                             ttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAtgt                                     >  7:297071/1‑149 (MQ=255)
                                                                                                             ttgATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAtgt                                     >  6:105034/1‑149 (MQ=255)
                                                                                                               gATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAtgt                                     >  1:298621/1‑147 (MQ=255)
                                                                                                               gATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAAtgt                                     <  2:298621/147‑1 (MQ=255)
                                                                                                                 tGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTc                                  <  5:105034/148‑1 (MQ=255)
                                                                                                                   gTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCAcc                               <  3:237888/149‑1 (MQ=255)
                                                                                                                    tGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCt                              >  5:219976/1‑149 (MQ=255)
                                                                                                                     gCCATTTTAGTTAATTTACCCTTACAAAATAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTc                                  >  3:37174/1‑144 (MQ=255)
                                                                                                                     gCCATTTTAGTTAATTTACCCTTACAAAATAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTc                                  >  3:32368/1‑144 (MQ=255)
                                                                                                                     gCCATTTTAGTTAATTTACCCTTACAAAATAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTc                                  <  4:32368/144‑1 (MQ=255)
                                                                                                                     gCCATTTTAGTTAATTTACCCTTACAAAATAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTc                                  <  4:37174/144‑1 (MQ=255)
                                                                                                                        aTTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGt                          <  7:368160/149‑1 (MQ=255)
                                                                                                                           ttAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGa                        <  3:254462/148‑1 (MQ=255)
                                                                                                                              gTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTg                     <  3:224927/148‑1 (MQ=255)
                                                                                                                              gTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATAACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTAAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGt                    >  1:336443/1‑149 (MQ=255)
                                                                                                                               ttAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTc                   >  2:142247/1‑149 (MQ=255)
                                                                                                                                 aaTTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATgtgt                                   <  1:204507/131‑1 (MQ=255)
                                                                                                                                 aaTTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATgtgt                                   >  2:204507/1‑131 (MQ=255)
                                                                                                                                 aaTTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTcc                  <  7:260425/148‑1 (MQ=255)
                                                                                                                                 aaTTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTc                   >  2:54309/1‑147 (MQ=255)
                                                                                                                                  aTTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTaa                                                                       <  6:41914/94‑1 (MQ=255)
                                                                                                                                  aTTTACCCTTACAAAACAAACAGATAAAATAAAAACATAAATCATTATCTTCTGTTTCTTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTAGTGAAGCCTCTATTATTCAGAAAAtgtg                                    >  6:285873/1‑129 (MQ=255)
                                                                                                                                  aTTTACCCTTAAAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCAAATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGGGAAGCCGCTATTATTCAGAAAAtgtg                                    <  5:285873/129‑1 (MQ=255)
                                                                                                                                     tACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTc              <  7:112409/148‑1 (MQ=255)
                                                                                                                                      aCCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCt            >  5:256810/1‑149 (MQ=255)
                                                                                                                                       cccTTACAAAACAAACAGAGAAAATAAAAACATCAGTCATTATCTTCTGTTTCGTTCCACACATCAGAACGCCTGACCTCAAGCTCTAATGACGTCGTGAATCCGCTATTATTCAGAAAATGTGTCACCTAAGTGATTGTCCATACCTg           >  6:115011/1‑149 (MQ=255)
                                                                                                                                        ccTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGc          <  6:219976/149‑1 (MQ=255)
                                                                                                                                          ttACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTCCCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGCTc        >  7:79901/1‑149 (MQ=255)
                                                                                                                                              aaaacaaaCAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCtatta                                                 <  2:336681/104‑1 (MQ=255)
                                                                                                                                              aaaacaaaCAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCtatta                                                 >  1:336681/1‑104 (MQ=255)
                                                                                                                                              aaaacaaaCAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGCTCGTc     >  6:298492/1‑148 (MQ=255)
                                                                                                                                                aacaaacaGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGCTCGTCTAt  >  1:214714/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGTGATAACGCTCTTTTGTCGTGTCAGTGATATAGCGGCTTGTACGCGCATGTGCGGCATGCTGGCATAAAGGACAATGAAACATCGCGAGCACCTCTTCCGGTTTTGTTGATGGTGCCATTTTAGTTAATTTACCCTTACAAAACAAACAGATAAAATAAAAACATCACTCATTATCTTCTGTTTCGTACCCCACATCAGAAAGCCTGACCTCAAGCTCTAATGACGTCGTGAAGCCGCTATTATTCAGAAAATGTGTCACCTTAGTGATTGTCCAGTCCTGCTCGTCTAT  >  NC_000913/2167435‑2167727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: