Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,253,194 G→A 7.3% L318F (CTT→TTT)  nupX ← nucleoside permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,253,1940GA 7.3% 169.4 / 2.8 55L318F (CTT→TTT) nupXnucleoside permease
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

AGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAA  >  NC_000913/2253050‑2253341
                                                                                                                                                |                                                                                                                                                   
aGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTc                                                                                                                                                 <  7:1186/149‑1 (MQ=255)
  ttAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGGTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCg                                                                                                                                                >  1:329321/1‑148 (MQ=255)
    aGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCa                                                                                                                                              <  5:65488/148‑1 (MQ=255)
     gCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCAt                                                                                                                                             >  6:353655/1‑148 (MQ=255)
            cGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAg                                                                                                                                     <  2:219333/149‑1 (MQ=255)
             gCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTCCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGt                                                                                                                                    <  6:81375/149‑1 (MQ=255)
                   cgcgAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCacac                                                                                                                              <  3:104125/149‑1 (MQ=255)
                                       caGTTTTAGCATCGAGAGTGCCAGCCGTTTTCAGATAGGGTTAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGa                                                                                                          <  7:277288/149‑1 (MQ=255)
                                         gTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGc                                                                                                        <  5:353655/149‑1 (MQ=255)
                                         gTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAAATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGc                                                                                                        >  2:341567/1‑149 (MQ=255)
                                         gTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAAATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGc                                                                                                        >  2:336313/1‑149 (MQ=255)
                                               gCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGa                                                                                                          >  8:80340/1‑141 (MQ=255)
                                               gCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGa                                                                                                          <  7:80340/141‑1 (MQ=255)
                                               gCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCcagcag                                                                                                  <  1:138123/149‑1 (MQ=255)
                                                           ccAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTaaaa                                                                                       <  1:268939/148‑1 (MQ=255)
                                                               ccGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCcaca                                                                                                                               >  3:137774/1‑104 (MQ=255)
                                                               ccGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCcaca                                                                                                                               <  4:137774/104‑1 (MQ=255)
                                                                               tGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGtt                                                                  >  7:344813/1‑149 (MQ=255)
                                                                                  gaAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCa                                                                >  1:13122/1‑148 (MQ=255)
                                                                                          gaTAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCAt                                                                     >  1:147438/1‑135 (MQ=255)
                                                                                          gaTAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCAt                                                                     <  2:147438/135‑1 (MQ=255)
                                                                                              aGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAAATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGc                                                   <  1:341567/149‑1 (MQ=255)
                                                                                              aGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAAATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGc                                                   <  1:336313/149‑1 (MQ=255)
                                                                                                 gACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGccac                                                >  2:196675/1‑149 (MQ=255)
                                                                                                    aaaTTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCaa                                             >  7:197775/1‑149 (MQ=255)
                                                                                                         cATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAAcgcc                                         <  8:197775/148‑1 (MQ=255)
                                                                                                               ttGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGat                                   >  8:356195/1‑148 (MQ=255)
                                                                                                                 gCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAaaccaaacca                                                     >  4:307245/1‑128 (MQ=255)
                                                                                                                 gCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAaaccaaacca                                                     <  3:307245/128‑1 (MQ=255)
                                                                                                                  ccAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataata                               >  8:269863/1‑149 (MQ=255)
                                                                                                                  ccAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataata                               >  4:133730/1‑149 (MQ=255)
                                                                                                                  ccAGTTGCTGGCCAATCCAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCCGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataata                               <  2:329321/149‑1 (MQ=255)
                                                                                                                   cAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCACTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCACACCAGCCACCAACGCCGCCGattatcc                              >  1:329734/1‑147 (MQ=255)
                                                                                                                    aGTTTCTGTCCAATCAAACTCCCGGCAAGATTCTCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAAACTAAAATGGCCTCCAGAGAGGAATGGTCAAAACCACACCAGCCACCCCCGCCGCCGATAATAcc                             >  6:73068/1‑149 (MQ=25)
                                                                                                                      tttCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGt                           <  8:344813/149‑1 (MQ=255)
                                                                                                                        tCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGtt                          <  7:356195/148‑1 (MQ=255)
                                                                                                                         cTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAACCCAGCCACCAACGCCGCCGATAATACCGTTaa                        >  5:263946/1‑149 (MQ=255)
                                                                                                                            tCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCCCCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCCGCGAGGCATGTTCAAAACCAAACCCGCCACCAACGCCGCCGATAATACCGTTAATc                      >  5:360308/1‑148 (MQ=255)
                                                                                                                               aaTCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTaa                        <  1:252978/143‑1 (MQ=255)
                                                                                                                               aaTCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTaa                        >  2:252978/1‑143 (MQ=255)
                                                                                                                                    aaCTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAg                                                                         >  5:52815/1‑89 (MQ=255)
                                                                                                                                    aaCTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAg                                                                         <  6:52815/89‑1 (MQ=255)
                                                                                                                                       tCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa                                 >  7:294421/1‑126 (MQ=255)
                                                                                                                                       tCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa                                 <  8:294421/126‑1 (MQ=255)
                                                                                                                                         ccGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTg         <  7:269863/148‑1 (MQ=255)
                                                                                                                                         ccGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCACTGGAGCCAGCAGTTAACCTAAAATGGACTCCAGCGAGGCATGTGCAAAACCAAACCAGCCTCCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGc        <  5:73068/149‑1 (MQ=255)
                                                                                                                                          cGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGc        >  2:292562/1‑148 (MQ=255)
                                                                                                                                           ggCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaa      <  1:196675/149‑1 (MQ=255)
                                                                                                                                            gCAAGATTCTCATCACTCCAGTCCACAGCCATCACCCACGCAAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCTAACCAGCAACCAACGCCGCCGATAATACCGTTAAt                       <  8:88994/131‑1 (MQ=255)
                                                                                                                                            gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAAt                       >  7:88994/1‑131 (MQ=255)
                                                                                                                                            gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAAc                   <  3:344682/135‑1 (MQ=255)
                                                                                                                                            gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAAc                   >  4:344682/1‑135 (MQ=255)
                                                                                                                                            gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaac     <  1:292562/149‑1 (MQ=255)
                                                                                                                                            gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACATAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaa      >  6:279726/1‑148 (MQ=255)
                                                                                                                                               aGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACaaa  >  6:166084/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAA  >  NC_000913/2253050‑2253341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑