Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,348,798	T→A	5.3%	intergenic (+30/+24)	yfaE → / ← inaA	ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,348,798	0	T	A	5.3%	250.3 / 3.1	76	intergenic (+30/+24)	yfaE/inaA	ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base T (34/38);  new base A (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

CGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCGACGA  >  NC_000913/2348655‑2348942                                                                                                                                                |                                                                                                                                                  cGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTc                                                                                                                                              <  7:81333/149‑1 (MQ=255)  gCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCt                                                                                                                                             <  5:257959/149‑1 (MQ=255)    cTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTa                                                                                                                                           >  4:198107/1‑149 (MQ=255)       gTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATc                                                                                                                                         <  3:152229/148‑1 (MQ=255)        ttGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTg                                                                                                                                       >  5:204766/1‑149 (MQ=255)          gCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                     >  2:140402/1‑149 (MQ=255)           cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc‑c                                                                                                                                   <  6:129008/149‑1 (MQ=255)           cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                     <  3:303761/148‑1 (MQ=255)           cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                     <  8:113152/148‑1 (MQ=255)           cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATAAGACGCATAAGCGTTGTATCTGGca                                                                                                                                    <  6:279444/149‑2 (MQ=39)            aGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc‑c                                                                                                                                   >  7:177992/1‑148 (MQ=255)              gTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTa                                                                                                                                 >  7:153868/1‑148 (MQ=255)               tCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTAc                                                                                                                                >  1:303669/1‑148 (MQ=255)                 aaGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAAc                                                                                                                             >  4:258303/1‑149 (MQ=255)                        cTGGATTGCCGAACCGTTAGCCTTTATTAAGCCGGGGGAAATTTTGCCCAGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACa                                                                                                                      >  2:315340/1‑149 (MQ=255)                         tGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACAt                                                                                                                     >  8:97375/1‑149 (MQ=255)                           gATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACAtcg                                                                                                                   >  4:350701/1‑147 (MQ=255)                           gATTGCCGAACCGTTAGCCTTTATTCAGCCGCGGTAAATTTTGCCCTGTTGTGGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACAt                                                                                                                     <  3:191521/147‑1 (MQ=255)                                ccGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgtag                                                                                                              <  8:153868/149‑1 (MQ=255)                                 cGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgtagt                                                                                                             <  5:219329/149‑1 (MQ=255)                                   aaCCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTaa                                                                                                           >  5:238281/1‑149 (MQ=255)                                     ccGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCACTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTTGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATa                                                                                                         <  4:202190/149‑1 (MQ=255)                                      cGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTCCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATAc                                                                                                        <  5:337568/149‑1 (MQ=255)                                      cGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGGGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATAc                                                                                                        <  7:336726/149‑1 (MQ=255)                                        ttAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGc                                                                                                      <  8:177992/149‑1 (MQ=255)                                             cTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATgg                                                                                                                   >  3:278150/1‑131 (MQ=255)                                             cTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATgg                                                                                                                   <  4:278150/131‑1 (MQ=255)                                             cTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCa                                                                                                 >  8:17725/1‑149 (MQ=255)                                              tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGAtt                                                                                                                         >  3:221025/1‑124 (MQ=255)                                              tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGAtt                                                                                                                         <  4:221025/124‑1 (MQ=255)                                                                 ttttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                             <  3:350701/149‑1 (MQ=255)                                                                   ttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                            <  1:140402/148‑1 (MQ=255)                                                                   ttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                            <  8:215823/148‑1 (MQ=255)                                                                       ccTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                     >  6:372862/1‑88 (MQ=255)                                                                       ccTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                     <  5:372862/88‑1 (MQ=255)                                                                        cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGGTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGAAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg                                                                      >  3:246196/1‑149 (MQ=255)                                                                        cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgta                                                                                                               <  5:178583/108‑1 (MQ=255)                                                                        cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgta                                                                                                               >  6:178583/1‑108 (MQ=255)                                                                         tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                            >  8:261431/1‑142 (MQ=255)                                                                         tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                            <  7:261431/142‑1 (MQ=255)                                                                              ttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCa                                                                <  1:315340/149‑1 (MQ=255)                                                                                gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg                                                                      >  6:248652/1‑141 (MQ=255)                                                                                gCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg                                                                      <  5:248652/141‑1 (MQ=255)                                                                                        aaaGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTc                                                      <  3:198107/149‑1 (MQ=255)                                                                                        aaaGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTc                                                      <  2:303669/149‑1 (MQ=255)                                                                                         aaGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTc                                                      >  3:195907/1‑148 (MQ=255)                                                                                           ggCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAg                                                   >  7:132313/1‑149 (MQ=255)                                                                                               atatTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTgg                                               >  8:232946/1‑149 (MQ=255)                                                                                                  ttGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATAtt                                                          >  5:76360/1‑135 (MQ=255)                                                                                                  ttGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATAtt                                                          <  6:76360/135‑1 (MQ=255)                                                                                                    gAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgta                                                                                                               <  6:253255/80‑1 (MQ=255)                                                                                                    gAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgta                                                                                                               >  5:253255/1‑80 (MQ=255)                                                                                                     aaaTCGAGGTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGAAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGaa                                          <  4:246196/148‑1 (MQ=255)                                                                                                     aaaTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAg                                         >  7:26950/1‑149 (MQ=255)                                                                                                          gagaTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCAt                                     <  8:26950/148‑1 (MQ=255)                                                                                                          gagaTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                    <  3:258303/149‑1 (MQ=255)                                                                                                           agaTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGa                                   <  8:132313/149‑1 (MQ=255)                                                                                                           agaTGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGa                                   >  3:228125/1‑149 (MQ=255)                                                                                                                  aaTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCg                            >  5:289951/1‑149 (MQ=255)                                                                                                                    ttGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                             <  1:240747/98‑1 (MQ=255)                                                                                                                    ttGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                             >  2:240747/1‑98 (MQ=255)                                                                                                                       gTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGAt                                  >  1:381969/1‑138 (MQ=255)                                                                                                                       gTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCACCTGGCGGAAGTCATGAt                                  <  2:381969/138‑1 (MQ=255)                                                                                                                       gTTGTAG‑GCCAGATAAGACGCATAAGCGTTGTATCTGGCACTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCa                                                                >  5:100892/1‑108 (MQ=18)                                                                                                                       gTTGTAG‑GCCAGATAAGACGCATAAGCGTTGTATCTGGCACTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCa                                                                <  6:100892/108‑1 (MQ=18)                                                                                                                        ttGTAG‑GCCAGATAAGACGCATAAGCGTTGTATCTGGCACTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTAt                      >  4:281283/1‑149 (MQ=37)                                                                                                                             gTGCCAGATACAGCGGGTATGCGTCTTAGCTGGG‑CTACAACGATTACATGGCGTAGTAATACGCTTTCGCCTGCTGCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGa                 >  8:317997/1‑149 (MQ=255)                                                                                                                             gTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGa                 <  7:232946/149‑1 (MQ=255)                                                                                                                                 cAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGc              >  8:275128/1‑148 (MQ=255)                                                                                                                                        aaCGCTTATGCGTCTTATCTGGC‑CTACAACGATTACCTGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAgcggcg      >  2:49177/1‑149 (MQ=255)                                                                                                                                        aaCGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCgta                                                                                                               <  8:12372/44‑1 (MQ=255)                                                                                                                                        aaCGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGCCGCAAgcggcg      >  6:52729/1‑149 (MQ=255)                                                                                                                                          cGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGcgac    <  7:275128/149‑1 (MQ=255)                                                                                                                                            cTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGcgacga  <  7:17725/149‑1 (MQ=255)                                                                                                                                             ttATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCaa            <  7:86741/138‑1 (MQ=255)                                                                                                                                             ttATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCaa            >  8:86741/1‑138 (MQ=255)                                                                                                                                                |                                                                                                                                                  CGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGC‑CTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCGACGA  >  NC_000913/2348655‑2348942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑