Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,524,438 A→T 5.3% G147G (GGT→GGA)  xapA ← purine nucleoside phosphorylase 2; nicotinamide 1‑beta‑D‑riboside synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,524,4380AT 5.3% 230.5 / 3.4 76G147G (GGT→GGA) xapApurine nucleoside phosphorylase 2; nicotinamide 1‑beta‑D‑riboside synthase
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base T (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

CGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCG  >  NC_000913/2524290‑2524582
                                                                                                                                                    |                                                                                                                                                
ggAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGa                                                                                                                                                  <  3:75594/148‑1 (MQ=255)
 gAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCCTTTGCCAGTGAGAAGAAGGGCTCGACAAAACGCTCATCGTTAAGAc                                                                                                                                                 >  2:320155/1‑149 (MQ=255)
 gAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGa                                                                                                                                                  <  2:79934/148‑1 (MQ=255)
 gAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTAGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGGTAAGAc                                                                                                                                                 >  2:314996/1‑149 (MQ=255)
   aaTTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGAccc                                                                                                                                               <  3:276790/149‑1 (MQ=255)
     ttCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACccac                                                                                                                                             <  3:51498/149‑1 (MQ=255)
       cGGCCCCGGATACGATACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACccacc                                                                                                                                            <  4:224317/148‑1 (MQ=255)
            ccGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGc                                                                                                                                      <  2:166509/149‑1 (MQ=255)
             cGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCg                                                                                                                                     >  5:282901/1‑149 (MQ=255)
                    gagaCGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGTCCCACCATCGGCGTACCggg                                                                                                                              >  1:25815/1‑149 (MQ=255)
                    gagaCGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCggg                                                                                                                              >  3:336734/1‑149 (MQ=255)
                        cGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAACCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATg                                                                                                                          <  3:11417/149‑1 (MQ=255)
                         gAACACGCCCTCCGTCAGAGGGAAACGCTCTTCGTTCGCCACTTTTTGTACCAGTGCGCGGGATTCCGCATCGTAGGCAGTTGCCAGTGAGAAGAAGCGCTCTCCAACACGATCATCGTTAAGACCCCCCATCGGCGTACCGGGCAggg                                                                                                                         >  1:123005/1‑149 (MQ=255)
                            cacGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgtgt                                                                                                                      <  5:132740/149‑1 (MQ=255)
                             acGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGtt                                                                                                                     <  1:353263/149‑1 (MQ=255)
                               gCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATg                                                                                                                          <  6:91882/142‑1 (MQ=255)
                               gCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATg                                                                                                                          >  5:91882/1‑142 (MQ=255)
                               gCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa                                                                                                                   >  3:158124/1‑149 (MQ=255)
                               gCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa                                                                                                                   >  4:306567/1‑149 (MQ=255)
                                 ccTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGata                                                                                                                 <  5:166601/149‑1 (MQ=255)
                                 ccTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGata                                                                                                                 >  7:135657/1‑149 (MQ=255)
                                         agagGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCttt                                                                                                         <  3:306567/149‑1 (MQ=255)
                                           agGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCa                                                                                                       <  8:348006/149‑1 (MQ=255)
                                               aaaCCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGTCCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGc                                                                                                   <  2:25815/149‑1 (MQ=255)
                                                aaCCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgtg                                                                                                                       >  8:148430/1‑128 (MQ=255)
                                                aaCCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGgtg                                                                                                                       <  7:148430/128‑1 (MQ=255)
                                                 aCCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGa                                                                                                 >  5:311695/1‑149 (MQ=255)
                                                  cccTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGata                                                                                                                 >  7:363059/1‑132 (MQ=255)
                                                  cccTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGata                                                                                                                 <  8:363059/132‑1 (MQ=255)
                                                     tcttctTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAg                                                                                             >  4:156435/1‑149 (MQ=255)
                                                     tcttctTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCAGCGTAGGCATTTTCCAGTGAGAAGAAGCGCTCTCCAAACCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCATTCAATGAGACCat                                                                                             >  4:192631/1‑148 (MQ=255)
                                                     tattcttTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGAATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTATCCAAAACGATAATCGTTAATACACCCCATCGACGTACAGCGCATGGTGTAGATATTATAATTCAATGCGACCAg                                                                                             >  2:312073/3‑149 (MQ=255)
                                                            tCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGctgc                                                                                        <  6:282901/147‑1 (MQ=255)
                                                              gCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCaa                                                                                                      <  1:72113/131‑1 (MQ=255)
                                                              gCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCaa                                                                                                      >  2:72113/1‑131 (MQ=255)
                                                               ccACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa                                                                                                                   <  8:343163/117‑1 (MQ=255)
                                                               ccACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGa                                                                                                                   >  7:343163/1‑117 (MQ=255)
                                                                cACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGAc                                                                                                <  5:80453/135‑1 (MQ=255)
                                                                cACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGAc                                                                                                >  6:80453/1‑135 (MQ=255)
                                                                 aCTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGcccc                                                                                 >  5:46784/1‑149 (MQ=255)
                                                                         tAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCg                                                                         <  6:46784/149‑1 (MQ=255)
                                                                           aCAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGa                                                                       <  4:158124/149‑1 (MQ=255)
                                                                               tGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCa                                                                   <  6:311695/149‑1 (MQ=255)
                                                                                    ggTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAg                                                              >  7:48347/1‑149 (MQ=255)
                                                                                    ggTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGGGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAg                                                              <  1:320155/149‑1 (MQ=255)
                                                                                    ggTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGGTAAGACCCACCATCGGCGGACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAg                                                              <  1:314996/149‑1 (MQ=255)
                                                                                           cGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCg                                                       >  6:326976/1‑149 (MQ=255)
                                                                                              aTCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGTCCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCAt                                                    >  2:186187/1‑149 (MQ=255)
                                                                                                 gTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTgg                                                 >  5:205512/1‑149 (MQ=255)
                                                                                                 gTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTg                                                  <  5:272294/148‑1 (MQ=255)
                                                                                                 gTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTg                                                  <  8:135657/148‑1 (MQ=255)
                                                                                                     gCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTgg                                                 <  8:109065/145‑1 (MQ=255)
                                                                                                     gCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTgg                                                 >  7:109065/1‑145 (MQ=255)
                                                                                                     gCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCa                                             <  5:326976/149‑1 (MQ=255)
                                                                                                       aTTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAg                                            >  1:54798/1‑148 (MQ=255)
                                                                                                            ccAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGtt                                                                                                                     >  5:60417/1‑70 (MQ=255)
                                                                                                            ccAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGtt                                                                                                                     <  6:60417/70‑1 (MQ=255)
                                                                                                             cAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGc                                                         >  1:171466/1‑129 (MQ=255)
                                                                                                             cAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGc                                                         <  2:171466/129‑1 (MQ=255)
                                                                                                               gTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTTTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGc                                   >  2:306800/1‑149 (MQ=255)
                                                                                                               gTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAg                                    >  6:186103/1‑148 (MQ=255)
                                                                                                               gTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGc                                   <  6:205512/149‑1 (MQ=255)
                                                                                                                   gaagaaGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCg                                >  8:190481/1‑148 (MQ=255)
                                                                                                                    aagaagCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCa                              <  5:160418/149‑1 (MQ=255)
                                                                                                                         gcgcTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCAtt                                                   >  4:75479/1‑123 (MQ=255)
                                                                                                                         gcgcTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCAtt                                                   <  3:75479/123‑1 (MQ=255)
                                                                                                                                   aaaCGATCATCGTTAAGTCCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTaaa               <  1:186187/149‑1 (MQ=255)
                                                                                                                                    aaCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGaa                                          <  5:61894/121‑1 (MQ=255)
                                                                                                                                    aaCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGaa                                          >  6:61894/1‑121 (MQ=255)
                                                                                                                                    aaCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAg              <  7:190481/149‑1 (MQ=255)
                                                                                                                                     aCGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAgg             <  1:312073/149‑1 (MQ=255)
                                                                                                                                         tcatcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTAc          >  2:181934/1‑148 (MQ=255)
                                                                                                                                            tcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGt                                      >  7:333369/1‑117 (MQ=255)
                                                                                                                                            tcGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGt                                      <  8:333369/117‑1 (MQ=255)
                                                                                                                                              gTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTc                                 <  2:89830/120‑1 (MQ=255)
                                                                                                                                              gTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTc                                 >  1:89830/1‑120 (MQ=255)
                                                                                                                                               ttAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGc   <  1:306800/149‑1 (MQ=255)
                                                                                                                                                tAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCg  >  8:293361/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGAAATTCGGCCCCGGATACGAGACGAACACGCCCTCCGTCAGAGGGAAACCCTCTTCTTTCGCCACTTTTTGTAACAGTGCGCGGTATTCCGCATCGTAGGCATTTGCCAGTGAGAAGAAGCGCTCTCCAAAACGATCATCGTTAAGACCCACCATCGGCGTACCGGGCATGGTGTTGATATGATCTTTCAATGCGACCAGGCTGCCTGCCCCCACTTCCGGACGCAGCGAGCCTGCCGCATTGGTGCAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCG  >  NC_000913/2524290‑2524582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑