Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,712,180 C→T 5.6% A195A (GCG→GCA trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,712,1800CT 5.6% 222.8 / 2.5 71A195A (GCG→GCAtrmNtRNA1(Val) (adenine(37)‑N6)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base T (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

TAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATC  >  NC_000913/2712032‑2712326
                                                                                                                                                    |                                                                                                                                                  
tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTcc                                                                                                                                                    >  1:264721/1‑149 (MQ=255)
tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTcc                                                                                                                                                    >  4:304024/1‑149 (MQ=255)
 aaaCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                                                                   >  6:298745/1‑149 (MQ=255)
 aaaCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                                                                                   >  4:234624/1‑149 (MQ=255)
         aaaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGcc                                                                                                                                                 <  8:276346/143‑1 (MQ=255)
         aaaaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGcc                                                                                                                                                 >  7:276346/1‑143 (MQ=255)
                   gggcatcgcCGTATTAGCTTCGGCATTGTTCTGGGTTGGCCCACGAAAGACTAACCGCTCCGTAAATCATTCGCCCCGCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAAc                                                                                                                                 <  2:350803/143‑1 (MQ=255)
                         cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAAt                                                                                                                           <  3:234624/149‑1 (MQ=255)
                         cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAAt                                                                                                                           <  1:130543/149‑1 (MQ=255)
                         cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAAt                                                                                                                           <  5:183977/149‑1 (MQ=255)
                                     ttCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               >  4:276821/1‑149 (MQ=255)
                                     ttCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               >  2:81915/1‑149 (MQ=255)
                                     ttCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               >  8:13108/1‑149 (MQ=255)
                                       cGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               <  5:298745/147‑1 (MQ=255)
                                         gAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTaa                                                                                                           >  7:5290/1‑149 (MQ=255)
                                         gAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTaa                                                                                                           >  2:65415/1‑149 (MQ=255)
                                          aaTAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAAt                                                                                                          <  1:65415/149‑1 (MQ=255)
                                              gTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCa                                                                                                      <  2:264721/149‑1 (MQ=255)
                                               ttCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAg                                                                                                     <  3:304024/149‑1 (MQ=255)
                                               ttCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAg                                                                                                     >  7:82307/1‑149 (MQ=255)
                                               ttCTGGGCTGGCCCACGAAGGACTCAGCGTTCGCTAAAGCATTCTCCCGCCTGCGGGGCGAATGCCAGCAGCACCCGCTGCGGCAGTCGCGCTTCGTGTTCCGGCACAGCCGTCCGCAAACGAAAATGCCAGCCCATGCTTAATGCCa                                                                                                      <  5:355928/148‑1 (MQ=255)
                                                         gCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                                                                           <  8:91793/149‑1 (MQ=255)
                                                         gCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                                                                           >  7:26215/1‑149 (MQ=255)
                                                           ccACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAAc                                                                                          >  5:86498/1‑148 (MQ=255)
                                                                    aCTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAAt                                                                                <  8:26215/149‑1 (MQ=255)
                                                                      tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTCATGCCAGCTCCGTAAAACCATTACCaa                                                                                 >  6:229160/1‑146 (MQ=255)
                                                                      tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTCATGCCAGCTCCGTAAAACCATTACCaa                                                                                 <  5:229160/146‑1 (MQ=255)
                                                                      tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTa                                                                                     >  6:14829/1‑142 (MQ=255)
                                                                      tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTa                                                                                     <  5:14829/142‑1 (MQ=255)
                                                                      tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCt                                                                              >  3:152463/1‑149 (MQ=255)
                                                                        aGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGTCAGCTCCGTAAAACCATTACCAATCt                                                                              <  7:89151/147‑1 (MQ=255)
                                                                        aGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCt                                                                              <  7:13108/147‑1 (MQ=255)
                                                                               gCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTgg                                                                       >  1:56619/1‑147 (MQ=255)
                                                                               gCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgca                                                                     <  1:81915/149‑1 (MQ=255)
                                                                                 tAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcagc                                                                   >  4:238965/1‑149 (MQ=255)
                                                                                       aTTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCGTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACg                                                             >  5:350163/1‑149 (MQ=255)
                                                                                           tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGTGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCAtt                                                                                      <  2:277367/120‑1 (MQ=255)
                                                                                           tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGTGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCAtt                                                                                      >  1:277367/1‑120 (MQ=255)
                                                                                           tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCCTGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAg                                                          >  3:21912/1‑148 (MQ=255)
                                                                                           tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCAAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAg                                                          >  4:33881/1‑148 (MQ=255)
                                                                                             ccGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCTGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGaa                                                        >  6:153918/1‑148 (MQ=255)
                                                                                             ccGCCTGCGGGGAGAATGCCAGCACCACCCGATGCGGCAGTCGCGCTTCGTTTTCTGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGAACCACAACGCAGaa                                                        >  6:148623/1‑148 (MQ=255)
                                                                                                ccTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAt                                                    <  2:56619/149‑1 (MQ=255)
                                                                                                 cTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               <  6:149334/89‑1 (MQ=255)
                                                                                                 cTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGc                                                                                                               >  5:149334/1‑89 (MQ=255)
                                                                                                 cTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAt                                                    >  1:287310/1‑148 (MQ=255)
                                                                                                 cTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCGTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAAt                                                    <  2:287310/148‑1 (MQ=255)
                                                                                                    cGGGGAGAATGCCAGCAGCTCCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAAGCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATcc                                                  <  8:82307/147‑1 (MQ=255)
                                                                                                    cGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATcc                                                  <  4:152463/147‑1 (MQ=255)
                                                                                                     ggggAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCctc                                               >  3:148104/1‑149 (MQ=255)
                                                                                                                cagccgCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACCTCCGTCCGCCAACGTAAATGCCAGCCCCTGCTTAATGCACGCGCCGGACAAGCATTCACGATCTGCTCTGGCAGCACTCCGCAGCAAAAGCCCTCTTCGGGAATGc                                    >  7:268316/1‑149 (MQ=255)
                                                                                                                  gcagcaCCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATccc                                                 <  1:39223/134‑1 (MQ=255)
                                                                                                                  gcagcaCCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATccc                                                 >  2:39223/1‑134 (MQ=255)
                                                                                                                      caCCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTccg                              >  1:156495/1‑149 (MQ=255)
                                                                                                                         ccGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTaa                                       <  7:177003/137‑1 (MQ=255)
                                                                                                                         ccGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTaa                                       >  8:177003/1‑137 (MQ=255)
                                                                                                                          cGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTccgccg                           >  2:38460/1‑148 (MQ=255)
                                                                                                                             tGCGGCAGTCGCGCTTCGTTTTCTGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGcaca                       <  5:153918/149‑1 (MQ=255)
                                                                                                                             tGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGcaca                       <  3:276821/149‑1 (MQ=255)
                                                                                                                              gCGGCAGTCGCGCTTCGTTTTCTGCCACATCCGTCCGCAAAGGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGcaca                       <  5:148623/148‑1 (MQ=255)
                                                                                                                                ggCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc                                            <  6:353548/125‑1 (MQ=255)
                                                                                                                                ggCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc                                            >  5:353548/1‑125 (MQ=255)
                                                                                                                                ggCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGt                    >  4:148597/1‑149 (MQ=255)
                                                                                                                                 gCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATg                                     >  7:231152/1‑131 (MQ=255)
                                                                                                                                 gCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATg                                     <  8:231152/131‑1 (MQ=255)
                                                                                                                                   aGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAg                 >  7:381801/1‑149 (MQ=255)
                                                                                                                                           ttCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCct                                                >  8:298819/1‑110 (MQ=255)
                                                                                                                                           ttCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCct                                                <  7:298819/110‑1 (MQ=255)
                                                                                                                                           ttCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc                                            >  5:247766/1‑114 (MQ=255)
                                                                                                                                           ttCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTc                                            <  6:247766/114‑1 (MQ=255)
                                                                                                                                             cGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATgg        <  8:5290/148‑1 (MQ=255)
                                                                                                                                             cGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATgg        <  4:148104/148‑1 (MQ=255)
                                                                                                                                              gTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGt      <  8:381801/149‑1 (MQ=255)
                                                                                                                                                    cGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATc  >  8:385802/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAAAATCCCTCTTCGGTAATGCACTCCGCCGCACAGGTCAGCAACGATGGGTGATC  >  NC_000913/2712032‑2712326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: