Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,831,080 C→T 8.5% V404M (GTG→ATG)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,831,0800CT 8.5% 140.6 / 2.2 47V404M (GTG→ATG) norRanaerobic nitric oxide reductase DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (23/20);  new base T (2/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCACCGCCGCCACTTCTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGG  >  NC_000913/2830939‑2831224
                                                                                                                                             |                                                                                                                                                
cGGGCACCGCCGCCACTTCTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTccc                                                                                                                                           <  2:16460/149‑1 (MQ=255)
          ctccACTTCTGGCGTCGGCAACGTCACATCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCTCTGCGGGTGGCTCTCGCCAGAACAACCGCACTATTAAAAGAATGTTCCAATTCGCGCACTTTTCCCCGAAAAATg                                                                                                                                  >  6:110326/3‑148 (MQ=255)
           gccACTTCTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGGTCGCGCACGTTTCCCGGAAAACTGTa                                                                                                                                <  3:264891/149‑1 (MQ=255)
            ccACTTCTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAg                                                                                                                               >  4:50578/1‑149 (MQ=255)
                  cTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTg                                                                                                                          <  1:41159/148‑1 (MQ=255)
                     gCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGt                                                                                                                      <  4:315391/149‑1 (MQ=255)
                                     cTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCgg                                                                                                      >  6:33832/1‑149 (MQ=255)
                                        aGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTACAGTAAATTTc                                                                                                               <  1:51072/137‑1 (MQ=255)
                                        aGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTACAGTAAATTTc                                                                                                               >  2:51072/1‑137 (MQ=255)
                                          gAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACt                                                                                                  >  4:175109/1‑148 (MQ=255)
                                                cAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATAc                                                                                            >  6:342650/1‑148 (MQ=255)
                                                   aaTGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTTATACCAcg                                                                                        >  4:352928/1‑149 (MQ=255)
                                                     tGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATa                                                                                             <  5:39414/142‑1 (MQ=255)
                                                     tGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATa                                                                                             >  6:39414/1‑142 (MQ=255)
                                                       ttGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCggg                                                                                    <  5:342650/149‑1 (MQ=255)
                                                                aaGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCTGGAGAGCCCCt                                                                           >  3:311132/1‑149 (MQ=255)
                                                                aaGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGga                                                                                   <  2:293311/141‑1 (MQ=255)
                                                                aaGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGga                                                                                   >  1:293311/1‑141 (MQ=255)
                                                                   aaTCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCATGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCAc                                                                                                   >  8:127009/1‑122 (MQ=255)
                                                                   aaTCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCATGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCAc                                                                                                   <  7:121431/122‑1 (MQ=255)
                                                                   aaTCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCATGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCAc                                                                                                   <  7:127009/122‑1 (MQ=255)
                                                                   aaTCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCATGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCAc                                                                                                   >  8:121431/1‑122 (MQ=255)
                                                                   aaTCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTg                                                                                                                                  <  4:242639/91‑1 (MQ=255)
                                                                        cTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCaaa                                                                   <  5:33832/149‑1 (MQ=255)
                                                                         ttCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTa                                                                                                                                <  6:17925/87‑1 (MQ=255)
                                                                         ttCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTa                                                                                                                                >  5:17925/1‑87 (MQ=255)
                                                                         ttCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATa                                                                                             >  6:10229/1‑122 (MQ=255)
                                                                         ttCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATa                                                                                             <  5:10229/122‑1 (MQ=255)
                                                                           cATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGa                                                                >  1:316442/1‑149 (MQ=255)
                                                                            aTCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGACAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCCCTTACTACCACGCGGGCGCGCCCCTGCCGCTACCGac                                                               >  8:373614/1‑149 (MQ=255)
                                                                                              tctcGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCTGTAAATTTCGCGCTCCGGCACTTAATACCAcgc                                                                                       <  1:367161/107‑1 (MQ=255)
                                                                                              tctcGCAAGAAATAAAGCCCGATGAAAAGCATGTTCCAGTTAGCGAACCTTTCCCGGAAAACTGTAGTGTTGCAGTAAATATCGCGCTACGGAACTAAATACCAcgc                                                                                       >  2:367161/1‑107 (MQ=255)
                                                                                                tcGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTTATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATAcc                                             <  3:352928/147‑1 (MQ=255)
                                                                                                tcGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATAcccc                                           >  5:85897/1‑149 (MQ=255)
                                                                                                  gCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATAcccc                                           <  6:85897/147‑1 (MQ=255)
                                                                                                    cAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCTAACGACACTGCTCGCAGAAATACCCCGcga                                       <  4:311132/149‑3 (MQ=255)
                                                                                                         cTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCcagcag                                   >  4:291414/1‑148 (MQ=255)
                                                                                                            ccGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAAt                                >  1:127701/1‑148 (MQ=255)
                                                                                                             cGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGa                              >  5:137255/1‑149 (MQ=255)
                                                                                                                   attaaTAGCATGTTCCAGTTCGCGCACGTTTCACGGAAAACTGAAGTGTTGCAGTAAATATCGCGCTCCTGAACTTAATACCACGCGGGAGAGCCCCTGCCGAAAACGACCCTGCTCGCAGAAATACCCCGCCAGCAGAATGAcatcat                        <  5:110326/146‑1 (MQ=255)
                                                                                                                           cATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGAc                             <  3:194621/136‑1 (MQ=255)
                                                                                                                           cATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGAc                             >  4:194621/1‑136 (MQ=255)
                                                                                                                           cATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGc                <  3:50578/149‑1 (MQ=255)
                                                                                                                               ttCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATAcccc                                           >  2:218649/1‑118 (MQ=255)
                                                                                                                               ttCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATAcccc                                           <  1:218649/118‑1 (MQ=255)
                                                                                                                                 ccAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACg           >  5:73497/1‑148 (MQ=255)
                                                                                                                                          gcACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGcggcgg  >  5:230527/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CGGGCACCGCCGCCACTTCTGGCGTCGGCAACGTCACCTCAGGAAAAGCAAAATGTTGCGCCTCAAGAATCACTTCATCGCCGCTGCGGGTGGCTCTCGCCAGAACTACCGCCCGATGAATAGCATGTTCCAGTTCGCGCACGTTTCCCGGAAAACTGTAGTGTTGCAGTAAATTTCGCGCTCCGGCACTTAATACCACGCGGGAGAGCCCCTGCCGCAAACGACACTGCTCGCAGAAATACCCCGCCAGCAGAATGACATCATCGCCCCGCTCACGCAGCGGCGG  >  NC_000913/2830939‑2831224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑