Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,043,920 T→C 5.0% C87R (TGT→CGT)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,043,9200TC 5.0% 446.6 / 7.0 138C87R (TGT→CGT) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base T (69/62);  new base C (3/4);  total (72/66)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCACCCTCTCCCACTTT  >  NC_000913/3043772‑3044066
                                                                                                                                                    |                                                                                                                                                  
tgACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt                                                                                                                                                    >  2:152320/1‑149 (MQ=255)
    gTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt                                                                                                                                                    >  4:63395/1‑145 (MQ=255)
    gTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt                                                                                                                                                <  4:54344/149‑1 (MQ=255)
     ttCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt                                                                                                                                               >  8:179149/1‑149 (MQ=255)
     ttCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt                                                                                                                                                >  2:349706/1‑148 (MQ=255)
       cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc                                                                                                                                              <  8:346010/148‑1 (MQ=255)
       cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc                                                                                                                                              <  1:349706/148‑1 (MQ=255)
       cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc                                                                                                                                              <  4:320442/148‑1 (MQ=255)
       cTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCAGGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc                                                                                                                                              <  4:810/148‑1 (MQ=255)
         gACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt                                                                                                                                                <  1:17390/144‑1 (MQ=255)
         gACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt                                                                                                                                                >  2:17390/1‑144 (MQ=255)
          aCCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGCCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa                                                                                                                                           >  2:290995/1‑148 (MQ=255)
            cGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt                                                                                                                                        >  3:52070/1‑149 (MQ=255)
               tGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAACGTTTTCGTACATcc                                                                                                                                      >  6:57861/1‑148 (MQ=255)
                 gcgcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc                                                                                                                                       >  2:169256/1‑145 (MQ=255)
                 gcgcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAtt                                                                                                                                   <  4:260409/149‑1 (MQ=255)
                   gcACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGc                                                                                                                                 <  7:179149/149‑1 (MQ=255)
                     acacGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCt                                                                                                                               >  7:160674/1‑149 (MQ=255)
                       acGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTgg                                                                                                                             <  4:52070/149‑1 (MQ=255)
                                    cgAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTc                                                                                                                <  8:174574/149‑1 (MQ=255)
                                         tCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa                                                                                                           <  6:55178/149‑1 (MQ=255)
                                          cACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg                                                                                                                              <  3:190184/129‑1 (MQ=255)
                                          cACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg                                                                                                                              >  4:190184/1‑129 (MQ=255)
                                          cACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAg                                                                                                          >  7:56210/1‑149 (MQ=255)
                                             cAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCt                                                                                                                               <  7:376568/125‑1 (MQ=255)
                                             cAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGc                                                                                                        >  8:23817/1‑148 (MQ=255)
                                               aagaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa                                                                                                           >  7:308247/1‑143 (MQ=255)
                                               aagaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa                                                                                                           <  8:308247/143‑1 (MQ=255)
                                               aagaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAACGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                                                                     >  8:130414/1‑149 (MQ=255)
                                               aagaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGATGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                                                                     >  5:28643/1‑149 (MQ=255)
                                                 gaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAtt                                                                                                                                   <  5:229614/117‑1 (MQ=255)
                                                 gaagTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                                                                    >  2:264912/1‑148 (MQ=255)
                                                   agTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg                                                                                                 <  7:23817/149‑1 (MQ=255)
                                                     tCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                                                                               <  1:152320/149‑1 (MQ=255)
                                                           cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCt                                                                                                                               >  6:81285/1‑111 (MQ=255)
                                                           cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc                                                                                          >  7:371639/1‑148 (MQ=255)
                                                           cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc                                                                                          >  1:385164/1‑148 (MQ=255)
                                                                     cTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag                                                                               <  8:56210/149‑1 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg                                                                                                 <  1:84983/128‑1 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg                                                                                                 >  2:84983/1‑128 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGc                                                                            >  1:131392/1‑149 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGc                                                                            >  1:136617/1‑149 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGc                                                                            >  3:221753/1‑149 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGc                                                                            >  5:23574/1‑149 (MQ=255)
                                                                        tCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATCTTTTCGTACATACATTGCCTGGACGCGCATTTTTCGAAAAGGCTATGATGCTCAGCCAAAAGAAGAAGGGc                                                                            >  2:250347/1‑149 (MQ=255)
                                                                            aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                                                                        <  3:305618/149‑1 (MQ=255)
                                                                            aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAACGTTTTCGTACATCCATCGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                                                                        <  5:57861/149‑1 (MQ=255)
                                                                               cATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc                                                                                                                                              <  3:62636/76‑1 (MQ=255)
                                                                                   aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa                                                                  <  1:264912/148‑1 (MQ=255)
                                                                                   aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa                                                                  <  4:221753/148‑1 (MQ=255)
                                                                                    aGCCGATGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                                                                 <  6:28643/148‑1 (MQ=255)
                                                                                     gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACga                                                               <  3:323220/149‑1 (MQ=255)
                                                                                       cGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAACGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatg                                                             <  7:130414/149‑1 (MQ=255)
                                                                                       cGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAACGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatg                                                             <  7:119431/149‑1 (MQ=255)
                                                                                         ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatgat                                                           <  4:76850/149‑1 (MQ=255)
                                                                                             tttttATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGt                                                       >  8:200237/1‑149 (MQ=255)
                                                                                                atATGGTCACTATAAGGAAGCCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCTTACATCCAATGCCTGTACGTGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc                                                     >  4:341419/2‑148 (MQ=255)
                                                                                                   tGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGAt                                                  <  7:2744/148‑1 (MQ=255)
                                                                                                     gTCACTATAAGGAAGACATCAAGCTATTTGCAGAAATGGGCTTGAATTGTTTTCGTACATCCATTGCCTGTACGCGCATTTTTCCTAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGaa                                               <  6:23574/149‑1 (MQ=255)
                                                                                                      tCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat                                                              >  4:40918/1‑133 (MQ=255)
                                                                                                      tCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat                                                              <  3:40918/133‑1 (MQ=255)
                                                                                                      tCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAAc                                              >  2:120138/1‑149 (MQ=255)
                                                                                                         cTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgc                                           <  1:257708/149‑1 (MQ=255)
                                                                                                         cTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgc                                           >  7:197740/1‑149 (MQ=255)
                                                                                                          tataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAAc                                                                                      >  8:1671/1‑105 (MQ=255)
                                                                                                          tataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgct                                          >  4:134104/1‑149 (MQ=255)
                                                                                                            taAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgct                                          <  6:110577/147‑1 (MQ=255)
                                                                                                              aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAGGGGCTGAAGTTCTAc                                                                 >  8:336398/1‑122 (MQ=255)
                                                                                                               ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAAt                                     <  1:290995/149‑1 (MQ=255)
                                                                                                                 aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATa                                    >  8:106285/1‑148 (MQ=255)
                                                                                                                      aTCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt                                                                      >  1:220891/1‑109 (MQ=255)
                                                                                                                      aTCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt                                                                      <  2:220891/109‑1 (MQ=255)
                                                                                                                      aTCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat                                                              >  6:330412/1‑117 (MQ=255)
                                                                                                                      aTCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat                                                              <  5:330412/117‑1 (MQ=255)
                                                                                                                       tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                                                                    >  8:388283/1‑110 (MQ=255)
                                                                                                                         aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatgat                                                           <  4:66612/117‑1 (MQ=255)
                                                                                                                         aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatgat                                                           <  2:35225/117‑1 (MQ=255)
                                                                                                                         aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatgat                                                           >  3:66612/1‑117 (MQ=255)
                                                                                                                         aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATc                             <  6:310388/147‑1 (MQ=255)
                                                                                                                         aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATc                             >  5:310388/1‑147 (MQ=255)
                                                                                                                          aGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa                                                                  >  7:98669/1‑109 (MQ=255)
                                                                                                                          aGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGaa                          >  6:201947/1‑149 (MQ=255)
                                                                                                                          aGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGaa                          >  8:140584/1‑149 (MQ=255)
                                                                                                                               tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCgg                      <  2:131392/148‑1 (MQ=255)
                                                                                                                               tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGt                     <  5:45742/149‑1 (MQ=255)
                                                                                                                                    cGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCa                >  8:350200/1‑149 (MQ=255)
                                                                                                                                     gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCAc               <  5:60777/149‑1 (MQ=255)
                                                                                                                                           ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCACCCtctc         >  3:243048/1‑149 (MQ=255)
                                                                                                                                             cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaaa                                      <  4:92157/118‑1 (MQ=255)
                                                                                                                                             cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaaa                                      >  3:92157/1‑118 (MQ=255)
                                                                                                                                             cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCACCCTCTccc       >  6:243276/1‑149 (MQ=255)
                                                                                                                                              ttCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCgg                      <  2:275544/133‑1 (MQ=255)
                                                                                                                                              ttCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCgg                      >  1:275544/1‑133 (MQ=255)
                                                                                                                                              ttCAAATGTTTTCGTACATCCATTGCCTGGACACGCATTTTTCAAAAAGGCGATGAAGCTCAGCCAAACGAAGAACGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTCAAATACAACATCTAACCGGTGATCACCCTCTCCCa      >  5:256299/1‑149 (MQ=255)
                                                                                                                                                 aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGAt                                                  >  8:374690/1‑102 (MQ=255)
                                                                                                                                                  aaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCGAAACGAAGAAGGGCTGAAGGTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCACCCTCTCCCACttt  >  4:256035/1‑149 (MQ=255)
                                                                                                                                                  aaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgctg                                         >  5:93313/1‑110 (MQ=255)
                                                                                                                                                  aaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgctg                                         <  6:93313/110‑1 (MQ=255)
                                                                                                                                                   aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCa                                                                                             <  1:64836/57‑1 (MQ=255)
                                                                                                                                                   aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCa                                                                                             >  2:64836/1‑57 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAATACAACATCGAACCGGTGATCACCCTCTCCCACTTT  >  NC_000913/3043772‑3044066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: