| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,045,437 | A→G | 5.6% | D155D (GAT→GAC) | ygfF ← | putative NAD(P)‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,045,437 | 0 | A | G | 5.6% | 217.9 / 2.8 | 72 | D155D (GAT→GAC) | ygfF | putative NAD(P)‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (34/33); new base G (2/2); total (37/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
GATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTAC > NC_000913/3045289‑3045583 | gATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTaa > 4:86859/1‑149 (MQ=255) aCGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAAc < 4:224580/149‑1 (MQ=255) gcgTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACat < 2:301784/149‑1 (MQ=255) gTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGCCTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTACTCAACatat > 3:22933/1‑149 (MQ=255) gTCCAGGCTCGCCCCCGCTGGCGTGCATTTATGTATAAATAAACCAAGGCCGCACACAGTTAAAGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGACCGGTGGTTAACGTATCAATCCCCACTTTCGATGCCGCGTAAAAAACagat > 6:5753/1‑149 (MQ=255) tCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATAtt > 8:172857/1‑149 (MQ=255) cccAGGCTCGCCGCCGCTGGCGTGCATTTCGTTATAAATAAACCCTGGCCGCACTCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATAtt < 5:66395/148‑1 (MQ=255) gCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCt > 6:185961/1‑149 (MQ=255) gccgccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGc < 4:257247/148‑1 (MQ=255) gccgccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGc > 4:315015/1‑148 (MQ=255) ccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAAcc > 3:7629/1‑149 (MQ=255) ccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAAcc < 4:7629/149‑1 (MQ=255) cGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGt < 2:120848/149‑1 (MQ=255) gTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGt > 3:201634/1‑148 (MQ=255) tGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGa < 4:202127/149‑1 (MQ=255) cATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAg < 4:22933/148‑1 (MQ=255) cATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAg < 3:86859/148‑1 (MQ=255) aTTTCGGTATAAATAATCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGc > 5:135260/1‑149 (MQ=255) gTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCg > 1:151261/1‑148 (MQ=255) ataaataaACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCg > 3:248681/1‑146 (MQ=255) ataaataaACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGaa < 4:248681/148‑1 (MQ=255) taaataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAaga < 5:185961/149‑1 (MQ=255) taaataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAaga < 4:201634/149‑1 (MQ=255) aataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAGTCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAgaga > 7:179307/1‑149 (MQ=255) aataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAGTCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAgaga > 7:173944/1‑149 (MQ=255) ataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGa < 4:103289/134‑1 (MQ=255) ataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGa > 3:103289/1‑134 (MQ=255) ataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGCAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAgaga > 5:267349/1‑148 (MQ=255) gCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAGTCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATa < 8:179307/149‑1 (MQ=255) gCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAGTCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATa < 8:173944/149‑1 (MQ=255) gCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGAt < 5:180244/148‑1 (MQ=255) aGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAgaga > 5:98229/1‑129 (MQ=255) aGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAgaga < 6:98229/129‑1 (MQ=255) gTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCAc > 1:88001/1‑149 (MQ=255) aaCGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGGACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGc > 3:84256/1‑149 (MQ=255) aCGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGa > 8:62273/1‑133 (MQ=255) aCGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGa < 7:62273/133‑1 (MQ=255) ggATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCAcc > 6:171711/1‑113 (MQ=255) ggATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCAcc < 5:171711/113‑1 (MQ=255) ggATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGccacca < 7:172857/149‑1 (MQ=255) tCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAttt < 3:315015/149‑1 (MQ=255) cccTGCGCGGCGACTTCCAGCGATAGTCCGGTTGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAtttt < 5:5753/148‑1 (MQ=255) cccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAttttt < 5:46914/149‑1 (MQ=255) ccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGCTGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAttt < 4:19743/146‑1 (MQ=255) ccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAttttt > 7:134928/1‑148 (MQ=255) ccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCAttt > 3:19743/1‑146 (MQ=255) cgcgGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAgc < 2:151261/148‑1 (MQ=255) cgcgGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAgc < 2:88001/148‑1 (MQ=255) gACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGAc < 4:219658/111‑1 (MQ=255) gACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGAc > 3:219658/1‑111 (MQ=255) ttCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGtt > 1:139913/1‑149 (MQ=255) ttCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGtt > 5:259898/1‑149 (MQ=255) aGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGATCCCAACCGGGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTAc > 2:371000/1‑149 (MQ=255) gtggtTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGgca > 7:269705/1‑149 (MQ=255) tggtTAACGTATCAATCGCCCCTTTCGATGCCGCGCAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGCCGATAGCTCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTAGCGCCTCGCGgcag < 6:267349/149‑1 (MQ=255) gtTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTg > 5:146257/1‑52 (MQ=255) gtTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTg < 6:146257/52‑1 (MQ=255) aCGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCagag > 6:260721/1‑148 (MQ=255) gTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGgcagca < 7:384143/143‑1 (MQ=255) gTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGgcagca > 8:384143/1‑143 (MQ=255) tATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGaaaa > 3:223879/1‑149 (MQ=255) aTCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCAc > 4:166379/1‑95 (MQ=255) aTCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCAc < 3:166379/95‑1 (MQ=255) aTCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGaaaa > 4:341228/1‑148 (MQ=255) aTCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGaaaa < 3:341228/148‑1 (MQ=255) tCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATccc > 3:49355/1‑149 (MQ=255) cccTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCa > 2:150058/1‑149 (MQ=255) cTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACg > 2:329539/1‑149 (MQ=255) tttCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGt > 8:115161/1‑149 (MQ=255) cgcgTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGaaa < 8:134928/149‑1 (MQ=255) gcgTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTcgcg > 6:355732/1‑113 (MQ=255) gcgTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTcgcg < 5:355732/113‑1 (MQ=255) tAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTAc < 6:135260/149‑1 (MQ=255) | GATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTAC > NC_000913/3045289‑3045583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |