Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,155,228 A→G 5.0% intergenic (‑10/‑127) yqhC ← / → yqhD transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,2280AG 5.0% 310.4 / 3.5 98intergenic (‑10/‑127)yqhC/yqhDtranscriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (44/49);  new base G (2/3);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

TCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGC  >  NC_000913/3155084‑3155373
                                                                                                                                                |                                                                                                                                                 
tCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActct                                                                                                                                               >  1:214606/1‑149 (MQ=255)
tCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActct                                                                                                                                               >  6:21171/1‑149 (MQ=255)
tCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActc                                                                                                                                                >  8:132907/1‑148 (MQ=255)
tCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActc                                                                                                                                                <  7:132907/148‑1 (MQ=255)
  cTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTgg                                                                                                                                             <  5:285051/149‑1 (MQ=255)
  cTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTg                                                                                                                                              <  8:328056/148‑1 (MQ=255)
   tGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActct                                                                                                                                               >  5:204283/1‑146 (MQ=255)
   tGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActct                                                                                                                                               <  6:204283/146‑1 (MQ=255)
     aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGgag                                                                                                                                           <  7:280046/148‑1 (MQ=255)
     aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                            <  8:86471/147‑1 (MQ=255)
     aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                            <  1:239050/147‑1 (MQ=255)
     aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                            <  3:185863/147‑1 (MQ=255)
     aCAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                            <  2:188694/147‑1 (MQ=255)
       aaaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGaaa                                                                                                                                        >  4:119865/1‑149 (MQ=255)
       aaaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGaaa                                                                                                                                        >  6:109145/1‑149 (MQ=255)
          cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAg                                                                                                                                     >  2:379067/1‑149 (MQ=255)
          cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAg                                                                                                                                     <  3:119865/149‑1 (MQ=255)
           tATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                                                                                                    >  8:201732/1‑149 (MQ=255)
            aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                                                                                                    >  4:325465/1‑148 (MQ=255)
             ttttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgctac                                                                                                                                  <  2:18830/149‑6 (MQ=255)
             ttttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgcta                                                                                                                                   <  2:353644/148‑5 (MQ=255)
               ttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCTCGTAATTTCTTACGAATAATGAGGCAATTTGATTGTTCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGc                                                                                                                                   >  1:123569/1‑146 (MQ=255)
                tCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGa                                                                                                                               >  2:172514/1‑149 (MQ=255)
                  tttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACa                                                                                                                             >  8:387938/1‑149 (MQ=255)
                  tttATTTTTCAGTTTATTAAATTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACa                                                                                                                             >  8:267058/1‑149 (MQ=255)
                   ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                            >  1:278172/1‑149 (MQ=255)
                      tttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                                                                                                         <  7:201732/149‑1 (MQ=255)
                      tttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                          <  1:165173/148‑1 (MQ=255)
                             gTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAt                                                                                                                  <  3:325465/149‑1 (MQ=255)
                                tattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                <  2:278172/148‑1 (MQ=255)
                                tattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATCTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                <  4:338304/148‑1 (MQ=255)
                                 attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgcta                                                                                                                                   >  1:168747/1‑124 (MQ=255)
                                 attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgcta                                                                                                                                   <  2:168747/128‑5 (MQ=255)
                                  ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                                                                                              >  8:51018/1‑148 (MQ=255)
                                  ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                                                                                              <  7:51018/148‑1 (MQ=255)
                                   taACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                            >  5:246799/1‑149 (MQ=255)
                                       tttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                           >  8:110910/1‑146 (MQ=255)
                                         tATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                <  6:303203/139‑1 (MQ=255)
                                         tATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                >  5:303203/1‑139 (MQ=255)
                                           tCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGcc                                                                                                    <  5:202260/149‑1 (MQ=255)
                                           tCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGc                                                                                                     >  3:50279/1‑148 (MQ=255)
                                            ccGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAt                                                                                                                  <  8:37295/134‑1 (MQ=255)
                                            ccGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAt                                                                                                                  >  7:37295/1‑134 (MQ=255)
                                                cAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                          >  4:246488/1‑122 (MQ=255)
                                                cAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                          <  3:246488/122‑1 (MQ=255)
                                                cAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGgc                                                                                               <  5:21171/149‑1 (MQ=255)
                                                  gCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGgcgc                                                                                             >  7:279229/1‑149 (MQ=255)
                                                        ggCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGa                                                                                       <  4:50279/149‑1 (MQ=255)
                                                        ggCAAATCTCTTCACGTTTAATATCGCGTAATTTCTTAGGAATAATTCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGATAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGa                                                                                       <  3:136254/149‑1 (MQ=255)
                                                         gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGAt                                                                                      <  3:135349/149‑1 (MQ=255)
                                                           aaaTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATca                                                                                    <  1:172514/149‑1 (MQ=255)
                                                             aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATcac                                                                                   >  4:199129/1‑148 (MQ=255)
                                                                tctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAAt                                                                                >  3:294558/1‑148 (MQ=255)
                                                                tctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAAt                                                                                >  6:307940/1‑148 (MQ=255)
                                                                 ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATg                                                                               >  8:125571/1‑148 (MQ=255)
                                                                  ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACaa                                                                                 <  7:10337/145‑1 (MQ=255)
                                                                  ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAAt                                                                                <  7:275020/146‑1 (MQ=255)
                                                                    cACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                            <  7:267058/148‑1 (MQ=255)
                                                                     aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACACTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                           >  5:171053/1‑116 (MQ=255)
                                                                     aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACACTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                           <  6:171053/116‑1 (MQ=255)
                                                                       gTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                        >  5:370653/1‑149 (MQ=255)
                                                                          tCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATc                                                                      <  7:125571/148‑1 (MQ=255)
                                                                                      aTTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGtt                                                          <  6:370653/148‑1 (MQ=255)
                                                                                       tttCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTtct                                                        >  1:21656/1‑149 (MQ=255)
                                                                                          cTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                      <  5:307940/148‑1 (MQ=255)
                                                                                           ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCGCTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGCGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGc                                                    >  2:94923/1‑149 (MQ=255)
                                                                                           ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGc                                                    >  5:21018/1‑149 (MQ=255)
                                                                                           ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGc                                                    >  7:174966/1‑149 (MQ=255)
                                                                                           ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGc                                                    >  5:41432/1‑149 (MQ=255)
                                                                                               gAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                      <  7:60656/143‑1 (MQ=255)
                                                                                               gAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                      >  8:60656/1‑143 (MQ=255)
                                                                                                    atGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCatat                                            <  5:109145/148‑1 (MQ=255)
                                                                                                    atGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATAtt                                           <  6:21018/149‑1 (MQ=255)
                                                                                                     tGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTg                                          <  7:387938/149‑1 (MQ=255)
                                                                                                       cGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGCGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGc                                        >  3:261953/1‑149 (MQ=255)
                                                                                                           aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGtt                                                          >  8:41202/1‑127 (MQ=255)
                                                                                                           aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGtt                                                          <  7:41202/127‑1 (MQ=255)
                                                                                                           aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                      >  5:349269/1‑131 (MQ=255)
                                                                                                           aaTTTGATGGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                      <  6:349269/131‑1 (MQ=255)
                                                                                                               tGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                     <  4:257698/112‑1 (MQ=255)
                                                                                                               tGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                     >  3:257698/1‑112 (MQ=255)
                                                                                                                gATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAg                               >  3:156437/1‑149 (MQ=255)
                                                                                                                 aTTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                              <  6:41432/149‑1 (MQ=255)
                                                                                                                 aTTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                              <  4:156437/149‑1 (MQ=255)
                                                                                                                 aTTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                              <  6:246799/149‑1 (MQ=255)
                                                                                                                     tgCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAg                           <  2:214606/148‑1 (MQ=255)
                                                                                                                          aaTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAACCATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGt                      >  6:141362/1‑148 (MQ=255)
                                                                                                                           aTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTaa                    <  3:199129/149‑1 (MQ=255)
                                                                                                                           aTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTaa                    <  1:379067/149‑1 (MQ=255)
                                                                                                                                   gCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac            >  7:138405/1‑149 (MQ=255)
                                                                                                                                     aTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACtt          >  3:279095/1‑149 (MQ=255)
                                                                                                                                          tcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGcc                                       <  8:15099/115‑1 (MQ=255)
                                                                                                                                          tcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAAt                   <  3:287797/135‑1 (MQ=255)
                                                                                                                                          tcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAAt                   >  4:287797/1‑135 (MQ=255)
                                                                                                                                            catcACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCTTTATCTGCCCTCATATTGGCACAGCAAATGGAGCAAGTAATTAACAACTTTAAtatt   >  3:249401/4‑146 (MQ=255)
                                                                                                                                            cAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGa                            >  4:212510/1‑124 (MQ=255)
                                                                                                                                            cAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGa                            <  3:212510/124‑1 (MQ=255)
                                                                                                                                             aGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGTGAGCCAGTGCGCTGGCGATCACAATGAAAAACTTCTGGCAGATCGTTCTCTGCCCTGATATTGGCGCAGCAAAGGGAGCATGTAATGAACATCTTTAATCTGc  <  2:123569/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGC  >  NC_000913/3155084‑3155373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: