Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,217,580 T→C 5.0% E499E (GAA→GAG aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,217,5800TC 5.0% 249.2 / 2.8 79E499E (GAA→GAGaerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATG  >  NC_000913/3217435‑3217726
                                                                                                                                                 |                                                                                                                                                  
ccGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTc                                                                                                                                                  <  5:4898/148‑1 (MQ=255)
ccGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTc                                                                                                                                                  <  7:167139/148‑1 (MQ=255)
        gACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCt                                                                                                                                         >  4:10174/1‑149 (MQ=255)
        gACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCt                                                                                                                                         >  5:232537/1‑149 (MQ=255)
             ttGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGAATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGgcgc                                                                                                                                    >  8:165697/1‑149 (MQ=255)
                  gcgcCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGgtgt                                                                                                                               >  6:246786/1‑149 (MQ=255)
                    gcCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt                                                                                                                             <  6:232537/149‑1 (MQ=255)
                      cTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCa                                                                                                                           >  8:176329/1‑149 (MQ=255)
                       ttATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCAc                                                                                                                          >  4:128494/1‑149 (MQ=255)
                         aTCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCa                                                                                                                        >  5:136899/1‑149 (MQ=255)
                          tCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATATCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCAt                                                                                                                       >  5:207369/1‑149 (MQ=255)
                          tCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCAt                                                                                                                       >  3:224259/1‑149 (MQ=255)
                                cTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGa                                                                                                                 <  2:323733/149‑1 (MQ=255)
                                      ccGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGgtgt                                                                                                                               >  1:143039/1‑129 (MQ=255)
                                      ccGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGgtgt                                                                                                                               <  2:143039/129‑1 (MQ=255)
                                      ccGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTg                                                                                                           >  5:74571/1‑149 (MQ=255)
                                      ccGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTg                                                                                                           >  1:1250/1‑149 (MQ=255)
                                             acacCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCActc                                                                                                    <  6:74571/149‑1 (MQ=255)
                                               acCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCa                                                                                                                                                <  4:251635/103‑1 (MQ=255)
                                               acCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCa                                                                                                                                                >  3:251635/1‑103 (MQ=255)
                                                 cccGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCtt                                                                                                >  8:68512/1‑149 (MQ=255)
                                                   cGCAGGCCTGACAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCCTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTc                                                                                               >  7:77136/1‑148 (MQ=255)
                                                   cGCAGGCCTGACAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCCTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTc                                                                                               >  7:71845/1‑148 (MQ=255)
                                                        gCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt                                                                                                                             >  3:200915/1‑113 (MQ=255)
                                                        gCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt                                                                                                                             <  4:200915/113‑1 (MQ=255)
                                                         ccTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAg                                                                                         >  8:381995/1‑148 (MQ=255)
                                                          cTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCt                                                                                       >  2:387936/1‑149 (MQ=255)
                                                           tGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTc                                                                                      >  1:108466/1‑149 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt                                                                                                                             >  4:119095/1‑108 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt                                                                                                                             <  3:119095/108‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTc                                                                                      <  3:10174/147‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCgg                                                                                    >  4:229983/1‑149 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGACATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTcc                                                                                              <  1:151404/139‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGACATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTcc                                                                                              >  2:151404/1‑139 (MQ=255)
                                                                aGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCa                                                                                  <  2:1250/148‑1 (MQ=255)
                                                                        cAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         <  4:224259/149‑1 (MQ=255)
                                                                        cAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         >  6:215895/1‑149 (MQ=255)
                                                                         aGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGGATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTggg                                                                        <  7:165697/149‑1 (MQ=255)
                                                                             tcgcatcAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCTCGGTGTTTAACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCGCGGCATTTTTCTGGTTGa                                                                     >  4:254119/1‑148 (MQ=255)
                                                                             tcgcatcAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         >  5:222721/1‑144 (MQ=255)
                                                                             tcgcatcAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         <  6:222721/144‑1 (MQ=255)
                                                                               gcatcAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACGAGCTCGGCATTTTTCTGGGTGATc                                                                   <  6:136899/148‑1 (MQ=255)
                                                                                catcAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAg                                                                 <  3:128494/149‑1 (MQ=255)
                                                                                 atcCGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCTTCACCGCGTCTTCCAGTCGGATGGCGCGGTGTTTCACCATCGGCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAgg                                                                <  1:267411/149‑1 (MQ=255)
                                                                                     ggCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGtt                                                              >  6:268844/1‑147 (MQ=255)
                                                                                     ggCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCCTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTaa                                                            <  8:71845/149‑1 (MQ=255)
                                                                                     ggCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCCTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTaa                                                            <  8:77136/149‑1 (MQ=255)
                                                                                      gCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAg                                                           >  7:235133/1‑149 (MQ=255)
                                                                                      gCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGACGACACATGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAg                                                           <  3:254119/149‑1 (MQ=255)
                                                                                        aTTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCt                                                         >  2:272692/1‑149 (MQ=255)
                                                                                              cTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCa                                                   >  8:236639/1‑149 (MQ=255)
                                                                                              cTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCa                                                   <  5:268844/149‑1 (MQ=255)
                                                                                               tCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAgg                                                                >  6:18478/1‑135 (MQ=255)
                                                                                               tCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAgg                                                                <  5:18478/135‑1 (MQ=255)
                                                                                                 cAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGACGGCTGGCGCCGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCACCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCAACCACTg                                                >  7:288982/1‑149 (MQ=255)
                                                                                                  aaCCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGc                                               <  7:236639/149‑1 (MQ=255)
                                                                                                         ggATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTc                                        <  8:235133/149‑1 (MQ=255)
                                                                                                           aTCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAg                                      <  7:381995/149‑1 (MQ=255)
                                                                                                            tCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         <  1:120234/113‑1 (MQ=255)
                                                                                                            tCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTgg                                                                         >  2:120234/1‑113 (MQ=255)
                                                                                                            tCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGtt                                                              >  5:339410/1‑124 (MQ=255)
                                                                                                            tCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGtt                                                              <  6:339410/124‑1 (MQ=255)
                                                                                                              cGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTCCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGc                                    <  6:358598/148‑1 (MQ=255)
                                                                                                                gCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTg                                  >  8:51695/1‑148 (MQ=255)
                                                                                                                   tACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGaaa                              <  5:246786/149‑1 (MQ=255)
                                                                                                                    aCCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAg                             >  1:20620/1‑149 (MQ=255)
                                                                                                                     tcGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGc                            <  6:207369/148‑1 (MQ=255)
                                                                                                                           aaTGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATc                       <  1:387936/148‑1 (MQ=255)
                                                                                                                            aTGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCgg                     >  7:134884/1‑149 (MQ=255)
                                                                                                                            aTGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCgg                     >  5:146964/1‑149 (MQ=255)
                                                                                                                             tGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGc                    <  7:51695/149‑1 (MQ=255)
                                                                                                                                aGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCAACCAGCTAGGCATTTTTCTGGGTCATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTg                 >  7:187062/1‑149 (MQ=255)
                                                                                                                                  tACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGtt               <  2:108466/149‑1 (MQ=255)
                                                                                                                                       tCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGc          >  5:220305/1‑149 (MQ=255)
                                                                                                                                       tCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGc          >  3:221843/1‑149 (MQ=255)
                                                                                                                                          ccGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGtt       <  3:229983/149‑1 (MQ=255)
                                                                                                                                              gTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAAt   <  6:146964/149‑1 (MQ=255)
                                                                                                                                              gTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAAt   <  6:220305/149‑1 (MQ=255)
                                                                                                                                               tCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATg  <  1:272692/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATG  >  NC_000913/3217435‑3217726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: