Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,232,611 C→T 6.3% A316V (GCT→GTT)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,232,6110CT 6.3% 200.0 / 2.4 63A316V (GCT→GTT) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (25/34);  new base T (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACG  >  NC_000913/3232463‑3232751
                                                                                                                                                    |                                                                                                                                            
ggcgCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGc                                                                                                                                              >  4:213358/1‑149 (MQ=255)
ggcgCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGc                                                                                                                                              <  2:369300/149‑1 (MQ=255)
ggcgCATATAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGc                                                                                                                                              >  2:306627/1‑149 (MQ=255)
     aTTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATg                                                                                                                                         <  7:213045/149‑1 (MQ=255)
                      gcAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCGGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCaa                                                                                                                          >  4:147915/1‑147 (MQ=255)
                        aaaCTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAgc                                                                                                                      <  2:262330/149‑1 (MQ=255)
                         aaCTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAgcg                                                                                                                     >  2:225441/1‑149 (MQ=255)
                            tGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGc                                                                                                                                              >  6:68067/1‑121 (MQ=255)
                            tGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGc                                                                                                                                              <  5:68067/121‑1 (MQ=255)
                             gAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAAt                                                                                                                 >  6:11598/1‑149 (MQ=255)
                                 ggCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCggg                                                                                                             <  7:364868/149‑1 (MQ=255)
                                 ggCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCgg                                                                                                              <  8:344798/148‑1 (MQ=255)
                                 ggCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCgg                                                                                                              >  7:344798/1‑148 (MQ=255)
                                    cACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACg                                                                                                          >  6:146567/1‑149 (MQ=255)
                                            gCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGAt                                                                                                   <  2:296643/148‑1 (MQ=255)
                                            gCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGAt                                                                                                   <  3:213358/148‑1 (MQ=255)
                                             cTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGAt                                                                                                   >  8:343586/1‑147 (MQ=255)
                                             cTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATga                                                                                                 <  2:309173/149‑1 (MQ=255)
                                             cTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATga                                                                                                 <  1:225441/149‑1 (MQ=255)
                                               gCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTACTGTCAAAAGCGCAATCGGGACGAGCCGATgaga                                                                                               <  2:384420/149‑1 (MQ=255)
                                                ccGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATgag                                                                                                <  5:146567/147‑1 (MQ=255)
                                                      gTAACCACCAACCGGATTAACGATCCGCGGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAacac                                                                                        <  3:147915/149‑1 (MQ=255)
                                                         accaccAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACtt                                                                                      <  5:11598/148‑1 (MQ=255)
                                                           caccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGa                                                                                                         <  1:68473/127‑1 (MQ=255)
                                                           caccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGa                                                                                                         >  2:68473/1‑127 (MQ=255)
                                                            accaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTAt                                                                                  >  3:196256/1‑149 (MQ=255)
                                                             ccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGAt                                                                                                   >  3:172337/1‑131 (MQ=255)
                                                             ccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGAt                                                                                                   <  4:172337/131‑1 (MQ=255)
                                                             ccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTAtt                                                                                 >  8:339442/1‑149 (MQ=255)
                                                             ccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTAtt                                                                                 <  1:69305/149‑1 (MQ=255)
                                                             ccaACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTAtt                                                                                 <  1:306627/149‑1 (MQ=255)
                                                               aaCCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTgg                                                                               <  7:248062/149‑1 (MQ=255)
                                                               aaCCGGAATAACGATCCGAAGCTTGACGACGATAATCTATCGCGCGGCGATGCCGATATGGTATCGATCGCGCGACCGTTTCTTGCTGATCCCGAGCTCCAGTCAAAAGCCCAAACGGGACGAGCCGATGAGATCACCACTAGTATTg                                                                                >  2:276466/1‑148 (MQ=255)
                                                                    gATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCa                                                                          <  7:343586/149‑1 (MQ=255)
                                                                        aaCGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAacac                                                                                        <  8:387385/131‑1 (MQ=255)
                                                                        aaCGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAacac                                                                                        >  7:387385/1‑131 (MQ=255)
                                                                        aaCGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAAt                                                                        >  4:38300/1‑147 (MQ=255)
                                                                           gATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGAc                                                                                                           >  6:29247/1‑109 (MQ=255)
                                                                           gATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGAc                                                                                                           <  5:29247/109‑1 (MQ=255)
                                                                                   ggTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGTTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGAt                                                                                              >  7:125433/1‑114 (MQ=255)
                                                                                   ggTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGTTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGAt                                                                                              <  8:125433/114‑1 (MQ=255)
                                                                                   ggTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGTTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGAt                                                                                              >  1:194409/1‑114 (MQ=255)
                                                                                   ggTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGTTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGAt                                                                                              <  2:194409/114‑1 (MQ=255)
                                                                                    gTTGCCGACGATATTGTCGCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACAGTTGTATTGGCTGCAATCAGGCCTGTCTCGa                                                          <  3:284023/149‑1 (MQ=255)
                                                                                         cgacgaTATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCaaa                                                     >  4:306019/1‑149 (MQ=255)
                                                                                         cgacgaTATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCaaa                                                     <  4:196256/149‑1 (MQ=255)
                                                                                          gacgaTATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAAt                                                    >  5:17435/1‑149 (MQ=255)
                                                                                              atatTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTc                                                <  3:38300/149‑1 (MQ=255)
                                                                                                 ttCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGtt                                             >  3:314690/1‑149 (MQ=255)
                                                                                                  tgtctcGCGCGGCGATGCCGATATTGTATCGATGGCGGGACCGTTTCTTGCTGATGCGGAGCTGCTGTCTTAAGCGCAATCGGGACGAGGCGTTGAGTTCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGtt                                             <  1:276466/146‑1 (MQ=255)
                                                                                                  tctctcGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTg                                            >  2:129989/1‑149 (MQ=255)
                                                                                                   ctctcGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTg                                            <  6:17435/148‑1 (MQ=255)
                                                                                                   ctctcGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTg                                            <  4:314690/148‑1 (MQ=255)
                                                                                                    tctcGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCaaaa                                                                                                                        >  7:149070/1‑71 (MQ=255)
                                                                                                    tctcGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCaaaa                                                                                                                        <  8:149070/71‑1 (MQ=255)
                                                                                                        gcgcgGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGc                                                                   <  4:218446/120‑1 (MQ=255)
                                                                                                        gcgcgGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGc                                                                   >  3:218446/1‑120 (MQ=255)
                                                                                                          gcggcgATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGt                                     >  4:3176/1‑148 (MQ=255)
                                                                                                             gcgATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCAcc                                 <  1:129989/149‑1 (MQ=255)
                                                                                                                 tGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGt                             <  3:3176/149‑1 (MQ=255)
                                                                                                                         tGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGt                      <  7:339442/148‑1 (MQ=255)
                                                                                                                           gTATCGATGGCGCGACCGTTTCTTCCTGAAGCGGAGCTGCTGTCAATAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCACGATCAAATCTTCGTTGGCAAAGTAACCTCGTGCCTGGTGaa                   >  5:289966/1‑149 (MQ=255)
                                                                                                                                gATGGCGTGAGCGATTCTTGCTGTTGCGGAGCTGATGTCAAAAGCGCAATAGGGACGAGCCTCTGAGATCATCACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGTATCCTc              <  6:289966/149‑1 (MQ=255)
                                                                                                                                      gcgACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGa                    <  2:303385/137‑1 (MQ=255)
                                                                                                                                      gcgACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGa                    >  1:303385/1‑137 (MQ=255)
                                                                                                                                      gcgACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCt        <  3:306019/149‑1 (MQ=255)
                                                                                                                                             tttCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACg  >  6:43900/1‑148 (MQ=255)
                                                                                                                                                 ttGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATcc                >  8:349975/1‑130 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GGCGCATTTAGCTGGGTCACGCGCAAACTGAAAGGCCACGTCTCGCTGCCGCTGGTAACCACCAACCGGATTAACGATCCGCAGGTTGCCGACGATATTCTCTCGCGCGGCGATGCCGATATGGTATCGATGGCGCGACCGTTTCTTGCTGATGCGGAGCTGCTGTCAAAAGCGCAATCGGGACGAGCCGATGAGATCAACACTTGTATTGGCTGCAATCAGGCCTGTCTCGATCAAATCTTCGTTGGCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACG  >  NC_000913/3232463‑3232751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: