Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,781 C→T 6.3% intergenic (‑37/+46) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,7810CT 6.3% 246.8 / 4.9 79intergenic (‑37/+46)ygjV/uxaAImp‑YgjV family inner membrane protein/altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (35/39);  new base T (3/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTC  >  NC_000913/3241637‑3241922
                                                                                                                                                |                                                                                                                                             
gACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGGCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTca                                                                                                                                           <  6:104553/149‑1 (MQ=255)
 agcGAAAGCTTCTTTTTGAAGCGACGTTCGTCTCGATTGAAAATTGTTGTGATAGCTATCAGACAGGCTATGTAACCCACGCCATGGGCCAGCCAATACGCGGTCATGATTAATCATTAGCAGGTATGGAAAAGCAAACGTCTCTTca                                                                                                                                           <  8:86482/146‑1 (MQ=255)
  ccGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTcaca                                                                                                                                         <  3:200298/149‑1 (MQ=255)
    gAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACAtt                                                                                                                                       <  1:12856/149‑1 (MQ=255)
         ctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTcataatgtg                                                                                                                                    >  5:364712/1‑140 (MQ=255)
         ctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCtt                                                                                                                                             >  1:146890/1‑138 (MQ=255)
         ctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCtt                                                                                                                                             >  1:141518/1‑138 (MQ=255)
         ctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCtt                                                                                                                                             <  2:146890/138‑1 (MQ=255)
         ctgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCtt                                                                                                                                             <  2:141518/138‑1 (MQ=255)
          tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                     >  1:287624/1‑145 (MQ=255)
          tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                     <  2:287624/145‑1 (MQ=255)
           gctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAc                                                                                                                                >  4:275520/1‑149 (MQ=255)
             tttttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCATAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                              >  2:114168/1‑149 (MQ=255)
             tttttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                              >  3:261787/1‑149 (MQ=255)
                   aaGCGTCGTTCGTCACGTTTGAAAATTGTTGTGATACCAATCAGAAATGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGttt                                                                                                                         <  6:364712/148‑1 (MQ=255)
                   aaGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGtttt                                                                                                                        >  8:2297/1‑149 (MQ=255)
                      cGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                     <  4:233030/149‑1 (MQ=255)
                          gTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaa                                                                                                                 <  3:176420/149‑1 (MQ=255)
                          gTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaa                                                                                                                 <  4:261787/149‑1 (MQ=255)
                           ttCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                <  3:275520/149‑1 (MQ=255)
                           ttCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                <  7:2297/149‑1 (MQ=255)
                               tCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACt                                                                                                            >  6:340226/1‑149 (MQ=255)
                                cACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATg                                                                                                                                    >  8:44511/1‑124 (MQ=255)
                                cACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATg                                                                                                                                    <  7:44511/124‑1 (MQ=255)
                                        aaaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                   >  3:283594/1‑149 (MQ=255)
                                        aaaaaTGTTGTGATACCAATAAGAAAGGCGATGCCACCCACGCACTGGGCCAGACAATACGCGGTAATGAGAAAACCGTCGCAGGAATGGAAAAGCAAACGGAGCTTCACATTATGAAACGAAGGTTGTTATTAAAAACTCATTTCGAc                                                                                                   >  1:103822/1‑149 (MQ=255)
                                         aaacTGTTGTGATACCAATCAGAAAGGCGATGACACACACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAACCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACt                                                                                                  <  4:5642/149‑1 (MQ=255)
                                          aaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                 >  1:84906/1‑149 (MQ=255)
                                           aaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGACAATACGCGGTAATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                 >  3:67938/1‑148 (MQ=255)
                                                 gtgAAACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGc                                                                                           <  3:182485/148‑1 (MQ=255)
                                                      aCCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTa                                                                                                                                      >  7:167579/1‑100 (MQ=255)
                                                      aCCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTa                                                                                                                                      <  8:167579/100‑1 (MQ=255)
                                                             aGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                >  6:325835/1‑115 (MQ=255)
                                                             aGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                <  5:325835/115‑1 (MQ=255)
                                                                 aGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGaa                                                                          <  7:380279/149‑1 (MQ=255)
                                                                     gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTg                                                                            >  7:168456/1‑143 (MQ=255)
                                                                     gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATc                                                                      <  2:84906/149‑1 (MQ=255)
                                                                       tGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATTATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    <  1:114168/149‑1 (MQ=255)
                                                                        gACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    >  4:201347/1‑148 (MQ=255)
                                                                         acacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCa                                                                  <  8:168456/149‑1 (MQ=255)
                                                                           acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    >  1:162310/1‑145 (MQ=255)
                                                                           acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    <  2:162310/145‑1 (MQ=255)
                                                                           acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCATTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGt                                                                >  7:145830/1‑149 (MQ=255)
                                                                             ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCTCATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTc                                                              >  6:360128/1‑149 (MQ=255)
                                                                             ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTCTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTc                                                              >  6:61839/1‑149 (MQ=255)
                                                                             ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTCCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    <  2:155185/143‑1 (MQ=255)
                                                                             ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTCCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                    >  1:155185/1‑143 (MQ=255)
                                                                             ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCGTAGCAGGTATGGAAAAGCAAACGCCGCTTCACATTATGAACCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGt                                                                >  2:288034/1‑147 (MQ=255)
                                                                                   gcTGGGCCAGCCAATCCTCTGTCATGCGACATCATTAGCAGGGATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACTACGCTTTTGAATATCGACATTTATCGGaa                                                        <  2:103822/148‑1 (MQ=255)
                                                                                   ccTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGaa                                                        <  3:201347/149‑1 (MQ=255)
                                                                                                      ggTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                     <  5:340226/149‑1 (MQ=255)
                                                                                                          aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                     >  4:9183/1‑65 (MQ=255)
                                                                                                          aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                     <  3:9183/65‑1 (MQ=255)
                                                                                                             aTAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                              >  5:90136/1‑149 (MQ=255)
                                                                                                              tAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                     <  6:186569/61‑1 (MQ=255)
                                                                                                                aaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                            <  6:90136/148‑1 (MQ=255)
                                                                                                                 aTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                   >  1:17596/1‑140 (MQ=255)
                                                                                                                 aTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                   <  2:17596/140‑1 (MQ=255)
                                                                                                                  tacTTAGCCTGTCTGGAAAAGCAAACGGCGCTGCACATTAGGAAAAGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACCCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAc                           <  1:213764/145‑1 (MQ=255)
                                                                                                                  tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                           <  1:261554/99‑1 (MQ=255)
                                                                                                                  tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                           >  2:261554/1‑99 (MQ=255)
                                                                                                                  tCCTTAGCAGGTATGGAAAAGCAAACGGCGCGTCACAGTATGATACGCCGTTGTTTATGAACAACTCATTTAGACTTTATAGCGTTACGCCGCGTTTGAAGATCGCAAGATCGCGGAAGTCGTTACGCTCTTTACATGTTTGCTTACCg                         >  1:68009/1‑149 (MQ=255)
                                                                                                                  accTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACATTATAGCGTTACGCCGCTTTAGAAGATCGACAGTTCGCGGAAGTCTTTACGCTCGTTACAGGTTTGCttcc                           >  2:213764/2‑145 (MQ=255)
                                                                                                                       aGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTgg                     >  6:370716/1‑148 (MQ=255)
                                                                                                                       aGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                      <  5:61839/147‑1 (MQ=255)
                                                                                                                        gCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                    <  4:67938/148‑1 (MQ=255)
                                                                                                                            gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                            >  2:83935/1‑120 (MQ=255)
                                                                                                                            gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                            <  1:83935/120‑1 (MQ=255)
                                                                                                                            gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                     <  6:258018/127‑1 (MQ=255)
                                                                                                                            gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                     >  5:258018/1‑127 (MQ=255)
                                                                                                                             tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                            >  6:192018/1‑135 (MQ=255)
                                                                                                                             tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                            <  5:192018/135‑1 (MQ=255)
                                                                                                                               tGGAAAAGCAAACGGCGCTTCTCATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa            <  5:360128/149‑1 (MQ=255)
                                                                                                                                 gAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAAc           <  4:283594/148‑1 (MQ=255)
                                                                                                                                 gAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAAc           >  2:98615/1‑148 (MQ=255)
                                                                                                                                    taaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg       >  8:174388/5‑149 (MQ=39)
                                                                                                                                      gCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgt     >  8:378610/1‑149 (MQ=255)
                                                                                                                                        aaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgtgt   <  7:174388/149‑1 (MQ=255)
                                                                                                                                         aaCGGCGTGTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTATGAATATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTAGGCAAACTCAAAGATGGTGTc  >  1:108560/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTC  >  NC_000913/3241637‑3241922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: