Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,335,746 A→G 6.0% I250T (ATC→ACC)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,335,7460AG 6.0% 203.9 / 3.2 67I250T (ATC→ACC) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACC  >  NC_000913/3335602‑3335891
                                                                                                                                                |                                                                                                                                                 
ggcgCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAAt                                                                                                                                               >  2:335213/1‑149 (MQ=255)
 gcgcGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAAtt                                                                                                                                              <  1:335213/149‑1 (MQ=255)
      gtacgtacGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGcc                                                                                                                                         <  6:246591/149‑1 (MQ=255)
         cgtacgtTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGTCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCcgc                                                                                                                                       <  1:328767/148‑1 (MQ=255)
          gtacgtTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCTAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCga                                                                                                                                     <  1:284088/149‑1 (MQ=255)
          gtacgtTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCga                                                                                                                                     <  2:297378/149‑1 (MQ=255)
                    aGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATcgccg                                                                                                                           <  5:266791/149‑1 (MQ=255)
                       cTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATcgccgccg                                                                                                                        >  6:188008/1‑149 (MQ=255)
                             aCGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGGTAATTTTGCCGCGAGAAATCGCCGCCGccac                                                                                                                    >  2:356831/1‑147 (MQ=255)
                                    ccATGCATATCCACGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAg                                                                                                            >  8:370323/1‑148 (MQ=255)
                                     cATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTa                                                                                                          >  5:8628/1‑149 (MQ=255)
                                      aTGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTAc                                                                                                         >  8:306012/1‑149 (MQ=255)
                                          atatCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGt                                                                                                     >  3:86654/1‑149 (MQ=255)
                                             tCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGGTAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGttt                                                                                                   <  1:356831/148‑1 (MQ=255)
                                              ccAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAg                                                                                                                 <  1:87153/133‑1 (MQ=255)
                                              ccAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAg                                                                                                                 >  2:87153/1‑133 (MQ=255)
                                               cAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGa                                                                                                >  3:289405/1‑149 (MQ=255)
                                               cAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCG‑CGCCGCCACCAGGAAAGTACCGGTTTCGa                                                                                                >  7:82283/1‑148 (MQ=255)
                                                aGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGa                                                                                                >  7:376730/1‑148 (MQ=255)
                                                 ggCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATa                                                                                              >  2:295751/1‑149 (MQ=255)
                                                      aTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCg                                                                                                       <  8:288064/135‑1 (MQ=255)
                                                      aTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCg                                                                                                       >  7:288064/1‑135 (MQ=255)
                                                      aTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATc                                                                                         >  8:260433/1‑149 (MQ=255)
                                                         cAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCgg                                                                                      >  5:45683/1‑149 (MQ=255)
                                                         cAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCgg                                                                                      <  6:8628/149‑1 (MQ=255)
                                                         cAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTATCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCgg                                                                                      >  2:153759/1‑149 (MQ=255)
                                                         cAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTATCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCgg                                                                                      <  1:153759/149‑1 (MQ=255)
                                                                cGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGATACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGa                                                                                  <  7:370323/146‑1 (MQ=255)
                                                                     aCTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATa                                                                          <  4:289405/149‑1 (MQ=255)
                                                                     aCTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATa                                                                          <  7:260433/149‑1 (MQ=255)
                                                                     aCTTCGATGTCCGCTCCAGCGTCACGCAGTTGCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATa                                                                          <  4:306423/149‑1 (MQ=255)
                                                                       ttCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGa                                                                        >  4:173005/1‑149 (MQ=255)
                                                                       ttCGATGTCCGCCCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGa                                                                            >  4:134776/1‑145 (MQ=255)
                                                                       ttCGATGTCCGCCCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGa                                                                            <  3:134776/145‑1 (MQ=255)
                                                                       ttCCATGTCCGCTCCATCGTCACGCAGTTTCGCCAGCACGGCGTAGAGAGTATCTGGCTGAGACTTACGGCATATAATTTTGACGCGAGAAATCGCCGCCGCCACCATGAAAGTAACGGTTTCGATACGATCCGCCAGAACGCGATAg                                                                         >  6:241203/1‑148 (MQ=255)
                                                                         cGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGac                                                                       <  8:376730/148‑1 (MQ=255)
                                                                             gTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGa                                                                            <  4:276583/139‑1 (MQ=255)
                                                                             gTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGa                                                                            >  3:276583/1‑139 (MQ=255)
                                                                              tCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATGGACAccgc                                                                  <  5:161204/148‑1 (MQ=255)
                                                                                cGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACcgccgc                                                               <  3:173005/149‑1 (MQ=255)
                                                                                  cTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCgccgcc                                                              >  5:240183/1‑148 (MQ=255)
                                                                                   tCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCt                                                             >  6:193562/1‑148 (MQ=255)
                                                                                   tCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCt                                                             >  5:192466/1‑148 (MQ=255)
                                                                                     cAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTaa                                                           <  4:86654/148‑1 (MQ=255)
                                                                                          tCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTTGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     <  1:32478/149‑1 (MQ=255)
                                                                                          tCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     <  6:45683/149‑1 (MQ=255)
                                                                                          tCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     <  2:194328/149‑1 (MQ=255)
                                                                                            aCGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTcc                                                    <  5:188008/148‑1 (MQ=255)
                                                                                            aCGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCG‑CGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCca                                                   <  8:82283/148‑1 (MQ=255)
                                                                                              gCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGttt                                                                                                   <  8:287495/99‑1 (MQ=255)
                                                                                              gCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGttt                                                                                                   >  7:287495/1‑99 (MQ=255)
                                                                                              gCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGaa                                                                                 >  5:70600/1‑117 (MQ=255)
                                                                                              gCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGaa                                                                                 <  6:70600/117‑1 (MQ=255)
                                                                                                    tCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     <  6:349593/139‑1 (MQ=255)
                                                                                                    tCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     >  5:349593/1‑139 (MQ=255)
                                                                                                      gCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTTAGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGAt                                         <  5:241203/149‑1 (MQ=255)
                                                                                                      gCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGGTAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGa                                          >  7:149773/1‑148 (MQ=255)
                                                                                                      gCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGGTAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGa                                          <  8:149773/148‑1 (MQ=255)
                                                                                                      gCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGa                                          <  7:306012/148‑1 (MQ=255)
                                                                                                      gCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGAt                                         <  5:193562/149‑1 (MQ=255)
                                                                                                          gCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     <  4:32526/133‑1 (MQ=255)
                                                                                                          gCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTc                                                     >  3:32526/1‑133 (MQ=255)
                                                                                                          gCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGac                                      >  7:258081/1‑148 (MQ=255)
                                                                                                          gCACGGCGTCGAGAGTATCTGGCTGCGCGATACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCCCCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGac                                      >  2:54516/1‑148 (MQ=255)
                                                                                                                                  gcgcGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCt              >  7:109555/1‑148 (MQ=255)
                                                                                                                                           ggCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCa    <  8:258081/149‑1 (MQ=255)
                                                                                                                                              aGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGACCGCGATAGACACCGCCGCCTAAACGTTCCACACCGTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCAcc  >  1:365234/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACC  >  NC_000913/3335602‑3335891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑