Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,383,296 T→C 5.5% intergenic (+29/+34) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,383,2960TC 5.5% 218.5 / 3.0 74intergenic (+29/+34)degS/mdhserine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base C (2/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

GAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGC  >  NC_000913/3383148‑3383438
                                                                                                                                                     |                                                                                                                                              
gagaCGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGCGCGCTCAAAACAAAAAACCGGAGt                                                                                                                                                >  2:299163/1‑149 (MQ=255)
         gATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTcc                                                                                                                                       >  6:22100/1‑149 (MQ=255)
                  gCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTtta                                                                                                                              <  4:209868/149‑1 (MQ=255)
                  gCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTtta                                                                                                                              <  3:165341/149‑1 (MQ=255)
                    ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTttat                                                                                                                             >  2:367297/1‑148 (MQ=255)
                    ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTttat                                                                                                                             >  4:229280/1‑148 (MQ=255)
                    ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCAACATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTatat                                                                                                                             >  1:11701/1‑148 (MQ=255)
                              ccTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAAt                                                                                                                  >  5:120793/1‑149 (MQ=255)
                                  gTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCaa                                                                                                               <  8:261342/148‑1 (MQ=255)
                                        tGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACtta                                                                                                        <  2:380901/149‑1 (MQ=255)
                                           tCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                     <  4:79618/149‑1 (MQ=255)
                                            cccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTaa                                                                                                    >  7:364083/1‑149 (MQ=255)
                                             ccTGTAGTAGTGATGCGTGATGATAAGCATTTAACGCTGCAGGTCACGATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                                   <  3:206754/149‑1 (MQ=255)
                                             ccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                                   <  4:354060/149‑1 (MQ=255)
                                              cTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGtttttt                                                                                                                               >  1:304470/1‑120 (MQ=38)
                                              cTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGtttttt                                                                                                                               <  2:304470/120‑1 (MQ=38)
                                                 tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                <  1:42984/116‑1 (MQ=255)
                                                 tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                >  2:42984/1‑116 (MQ=255)
                                                 tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGaa                                                                                                <  8:136052/148‑1 (MQ=255)
                                                   gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAActc                                                                                             >  8:280873/1‑149 (MQ=255)
                                                   gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGTTTTttgttttga                                                                                                                        <  7:122311/122‑5 (MQ=37)
                                                   gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGTTTTttgttttga                                                                                                                        >  8:122311/1‑118 (MQ=37)
                                                      gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                >  1:146487/1‑111 (MQ=255)
                                                      gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                <  2:146487/111‑1 (MQ=255)
                                                      gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                     >  1:305298/1‑138 (MQ=255)
                                                      gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                     <  2:305298/138‑1 (MQ=255)
                                                      gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGCGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTc                                                                                          <  1:299163/149‑1 (MQ=255)
                                                           gCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGcc                                                                                       >  5:52543/1‑147 (MQ=255)
                                                             gTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAgg                                                                                   >  1:288436/1‑149 (MQ=255)
                                                                  gatAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGa                                                                               >  5:165378/1‑148 (MQ=255)
                                                                      aGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtct                                                                          >  7:30589/1‑149 (MQ=255)
                                                                       gCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctt                                                                         >  7:18044/1‑149 (MQ=255)
                                                                       gCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctt                                                                         >  3:21935/1‑149 (MQ=255)
                                                                         aGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttc                                                                       <  8:18044/149‑1 (MQ=255)
                                                                              aCGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCa                                                                   <  6:120793/148‑1 (MQ=255)
                                                                              aCGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTAATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAg                                                                  >  1:132918/1‑149 (MQ=255)
                                                                                gCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCg                                                                >  8:99758/1‑149 (MQ=255)
                                                                                  tGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTAATATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTa                                                              >  3:173870/1‑149 (MQ=255)
                                                                                     aGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtcttt                                                                        <  4:31067/136‑1 (MQ=255)
                                                                                     aGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtcttt                                                                        >  3:31067/1‑136 (MQ=255)
                                                                                       gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAg                                                         <  2:11701/149‑1 (MQ=255)
                                                                                          aCCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAAGTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTCTCCAGCa                                                       >  5:5409/1‑148 (MQ=255)
                                                                                            cATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttctt                                                                     >  8:333238/1‑132 (MQ=255)
                                                                                            cATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttctt                                                                     <  7:333238/132‑1 (MQ=255)
                                                                                             aTTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATAcc                                                   <  5:22100/149‑1 (MQ=255)
                                                                                              ttCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCt                                                  >  5:54783/1‑149 (MQ=255)
                                                                                                 aGGAATATCCGGCAACCAATTAAGTCTTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGTTTTTTTATTATCCGATAATAAATTACTTATTAAAGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCATCATaaatta                                                >  8:192865/1‑143 (MQ=255)
                                                                                                 aGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCg                                                                                                                       >  7:267582/1‑77 (MQ=255)
                                                                                                 aGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCg                                                                                                                       <  8:267582/77‑1 (MQ=255)
                                                                                                   gAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAAACCGGAGGGTGTGCGCCGGTTTTTTTTTATCCGCTATTCAATTCGTTATTAACGAAGTCTTCGCCCAGGGCGATAGCTTTCTTCAGCGTATCCAGCATACCTTc                                                >  1:247820/1‑147 (MQ=255)
                                                                                                      tatCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgcgc                                          >  3:102858/1‑149 (MQ=255)
                                                                                                        tCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGt                                        >  7:248561/1‑149 (MQ=255)
                                                                                                          cGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTc                                      <  3:229280/149‑1 (MQ=255)
                                                                                                           ggCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCt                                     <  8:30589/149‑1 (MQ=255)
                                                                                                              aaCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGtt                                  <  4:21935/149‑1 (MQ=255)
                                                                                                              aaCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTAATATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGtt                                  <  4:173870/149‑1 (MQ=255)
                                                                                                               aCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTc                                 <  7:280873/149‑1 (MQ=255)
                                                                                                               aCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTc                                 <  6:52543/149‑1 (MQ=255)
                                                                                                                ccAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCa                                <  1:367297/149‑1 (MQ=255)
                                                                                                                    ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgc                                            >  3:162586/1‑133 (MQ=255)
                                                                                                                    ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgc                                            <  4:162586/133‑1 (MQ=255)
                                                                                                                    ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATg                            >  3:342674/1‑149 (MQ=255)
                                                                                                                              cgcTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAggg                   <  8:364083/148‑1 (MQ=255)
                                                                                                                                 tCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTAcc               >  2:144345/1‑149 (MQ=255)
                                                                                                                                 tCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTAcc               >  8:384508/1‑149 (MQ=255)
                                                                                                                                     aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGat                                                                              >  6:95501/1‑82 (MQ=255)
                                                                                                                                     aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGat                                                                              <  5:95501/82‑1 (MQ=255)
                                                                                                                                     aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGa             >  3:174037/1‑147 (MQ=255)
                                                                                                                                     aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATa           <  7:99758/149‑1 (MQ=255)
                                                                                                                                         aaaaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt        >  2:170401/1‑149 (MQ=255)
                                                                                                                                         aaaaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt        >  3:7262/1‑149 (MQ=255)
                                                                                                                                           aaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAttt      <  6:54783/149‑1 (MQ=255)
                                                                                                                                             aaCCGGAGGCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTAc    <  2:247820/149‑1 (MQ=255)
                                                                                                                                              aCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACg   >  3:167451/1‑149 (MQ=255)
                                                                                                                                               caggAGTCTGTGCGCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCCGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGc  <  6:5409/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGC  >  NC_000913/3383148‑3383438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: