Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,514,351 A→G 6.4% D13D (GAT→GAC)  gph ← phosphoglycolate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,514,3510AG 6.4% 183.8 / 3.6 62D13D (GAT→GAC) gphphosphoglycolate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base G (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

CGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAT  >  NC_000913/3514209‑3514498
                                                                                                                                              |                                                                                                                                                   
cgcgcTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCaaaa                                                                                                                                               <  8:69974/149‑1 (MQ=255)
cgcgcTCATGCAGCACATCTGCGCCGTGACCAATCCAGGTAATAACGCGGTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCTCAGCAGCAAGACCAGTAGCACTGTCGACCAGCGTACCATCAAGATCaaaa                                                                                                                                               <  2:277377/149‑1 (MQ=255)
 gcgcTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAg                                                                                                                                              >  6:3390/1‑149 (MQ=255)
       caccAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAc                                                                                                                                          <  7:118942/144‑1 (MQ=255)
       cATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAc                                                                                                                                          <  3:104302/147‑1 (MQ=255)
           aGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAcgccgc                                                                                                                                     <  2:63257/148‑1 (MQ=255)
                aTCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCATGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAatat                                                                                                                               <  5:196707/149‑1 (MQ=255)
                   tGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAcgccgc                                                                                                                                     >  8:70437/1‑140 (MQ=255)
                   tGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAcgccgc                                                                                                                                     <  7:70437/140‑1 (MQ=255)
                     cgcCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGATCACTGTGGACCAGCGTACCATCAATATCAAAAGCGACGCCGCGAATATCt                                                                                                                             <  4:336154/146‑1 (MQ=255)
                     cgcCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCtt                                                                                                                            <  2:210590/147‑1 (MQ=255)
                            aCCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCaaaa                                                                                                                                               >  4:297868/1‑121 (MQ=255)
                            aCCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCaaaa                                                                                                                                               <  3:297868/121‑1 (MQ=255)
                               aaTCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCa                                                                                                                <  8:286558/149‑1 (MQ=255)
                                aTCCAGGTAATAACGCGTTCTTCACCTGAGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCAt                                                                                                               <  8:166563/149‑1 (MQ=255)
                                 tCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCCCTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCAt                                                                                                               >  1:148919/1‑148 (MQ=255)
                                   cAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGa                                                                                                             <  3:235710/148‑1 (MQ=255)
                                   cAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGAc                                                                                                            <  4:331318/149‑1 (MQ=255)
                                    agttaataaGGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGGGACGCCGCGAATATCTTCAAACTTAtt                                                                                                                  <  6:30321/139‑1 (MQ=255)
                                    aGGAAATAACGCGTTCTTCACCTGCGACGCGCAACTCCAGCTCATACAGCTCCAAATCTACCGCAGCAGCAAGACCAGGAGCACTGACGACCAGAGTACCATCAAGATCAAAAGAGACGCCTCGAATATCATCAAACTTAtt                                                                                                                  >  5:30321/1‑142 (MQ=255)
                                        aataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTAcc                                                                                                       >  2:69854/1‑149 (MQ=255)
                                         ataaCGCGTTCTTCACCTGCGACGGGCAAGTCCAGCGCATACAGCGCCATCTCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCt                                                                                                      <  7:273293/149‑1 (MQ=255)
                                         ataaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCt                                                                                                      <  2:93733/149‑1 (MQ=255)
                                          taaCGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCtt                                                                                                     <  1:69854/149‑1 (MQ=255)
                                             cgcgTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGc                                                                                                  <  5:3390/149‑1 (MQ=255)
                                                          tGCGACGGGCAACTCCAGCGCCTACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACGGTCGCCCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTgcg                                                                                      >  2:210347/1‑148 (MQ=255)
                                                              aCGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCAttt                                                                                 >  8:254653/1‑149 (MQ=255)
                                                               cGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTtc                                                                                <  3:116339/149‑1 (MQ=255)
                                                                       tCCAGCGCATACAGCGCCATATCTACC‑‑‑GCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtt                                                                     >  3:172900/1‑149 (MQ=255)
                                                                          aGCGCACACAGCGCCATATCTACCGCCGCAGCAAGACCAGGAGCACTGTCGGCCAGCGTACCCTCAAGATCAAAAGCGACGCCGCGAATGTCTTCAAACTTATTCATGACTTACCTTTGCCAGGTGACTGCGCATTTCATCAATGACtg                                                                     >  6:67719/1‑148 (MQ=255)
                                                                              cATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTGATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACtttttt                                                                 >  6:318323/1‑149 (MQ=255)
                                                                                  cAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTa                                                              <  7:254653/148‑1 (MQ=255)
                                                                                    gcgcCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTc                                                           <  2:148919/149‑1 (MQ=255)
                                                                                              tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAc                                                                                                                                          >  1:139360/1‑60 (MQ=255)
                                                                                              tACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGAc                                                                                                                                          <  2:139360/60‑1 (MQ=255)
                                                                                                ccGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTGATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGaa                                               <  5:318323/149‑1 (MQ=255)
                                                                                                ccGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCg                                                 >  1:36928/1‑147 (MQ=255)
                                                                                                   cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATTAATGACTTTTTTGTAGTctgg                                                        >  7:242128/1‑137 (MQ=255)
                                                                                                   cagcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATTAATGACTTTTTTGTAGTctgg                                                        <  8:242128/137‑1 (MQ=255)
                                                                                                        gcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGcc                                        >  5:87924/1‑148 (MQ=255)
                                                                                                             aCCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCg                                  >  6:215366/1‑149 (MQ=255)
                                                                                                               cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGCATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGCACCGGc                                >  6:218622/1‑149 (MQ=255)
                                                                                                               cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                >  6:246139/1‑149 (MQ=255)
                                                                                                               cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                >  8:108141/1‑149 (MQ=255)
                                                                                                               cAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGc                                >  3:202166/1‑149 (MQ=255)
                                                                                                                 ggAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcga                              <  4:202166/149‑1 (MQ=255)
                                                                                                                  gAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGcgac                             >  8:336018/1‑149 (MQ=255)
                                                                                                                          tCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACatat                     <  3:128875/149‑1 (MQ=255)
                                                                                                                          tCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACat                       <  5:246139/147‑1 (MQ=255)
                                                                                                                            gACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATcc                   <  2:36928/149‑1 (MQ=255)
                                                                                                                                 gCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcc              >  3:20065/1‑149 (MQ=255)
                                                                                                                                   gTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccg            >  6:325510/1‑149 (MQ=255)
                                                                                                                                    tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGcgcccgc           <  4:172900/149‑1 (MQ=255)
                                                                                                                                       cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCccgccg         >  8:215599/1‑148 (MQ=255)
                                                                                                                                       cATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCccgccg         >  3:248249/1‑148 (MQ=255)
                                                                                                                                        aTCAAGGTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                          <  3:129852/98‑1 (MQ=255)
                                                                                                                                        aTCAAGGTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                          <  3:134926/98‑1 (MQ=255)
                                                                                                                                        aTCAAGGTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                          >  4:134926/1‑98 (MQ=255)
                                                                                                                                        aTCAAGGTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTct                                                          >  4:129852/1‑98 (MQ=255)
                                                                                                                                           aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGc     >  1:266690/1‑148 (MQ=255)
                                                                                                                                           aaGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGc     >  3:330434/1‑148 (MQ=255)
                                                                                                                                             gATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAt  <  1:210347/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CGCGCTCCATCAGAACATCTGCGCCGTTACCAATCCAGGTAATAACGCGTTCTTCACCTGCGACGGGCAACTCCAGCGCATACAGCGCCATATCTACCGCAGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGAT  >  NC_000913/3514209‑3514498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑