Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,575,191 G→A 5.5% V41M (GTG→ATG)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,575,1910GA 5.5% 232.1 / 2.2 73V41M (GTG→ATG) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

CGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCC  >  NC_000913/3575046‑3575339
                                                                                                                                                 |                                                                                                                                                    
cgGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGc                                                                                                                                                   <  2:124281/149‑1 (MQ=255)
           aGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCt                                                                                                                                         <  8:124924/148‑1 (MQ=255)
           aGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCt                                                                                                                                         >  8:34514/1‑148 (MQ=255)
           aGGGAATTTGAGTTATGAAAGAAATCATTTATGCAGCAGATTTATTGATCCTGATTATGGATCCGCTCGGAAACCAACCTATTTTCATGTACGTACTGAAACATACTGAACCGAAAAGACGGCGGGAAATCATTGTGCGagagatcct                                                                                                                                         >  8:288952/1‑143 (MQ=255)
            gggAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTa                                                                                                                                       >  3:308346/1‑149 (MQ=255)
                 tttGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGct                                                                                                                                  >  3:116066/1‑149 (MQ=255)
                    gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCtatc                                                                                                                               >  1:369829/1‑149 (MQ=255)
                      gTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                                                                             <  4:157731/149‑1 (MQ=255)
                          tgaatgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGt                                                                                                                          <  8:137811/148‑1 (MQ=255)
                             atgaaATCATTTCTGCAGCAGTTTTATTGCTCCTGATTATGGAGCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGACCCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATg                                                                                                                      <  1:245152/149‑1 (MQ=255)
                             atgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATg                                                                                                                      >  8:217718/1‑149 (MQ=255)
                              tgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGc                                                                                                                     <  7:34514/149‑1 (MQ=255)
                                aaaTCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTg                                                                                                                   <  1:222720/149‑1 (MQ=255)
                                          tGCAGCAGTTTTATAGATCCTGATTATGGATCCTCTCGGAAACCTACCTATTTTCATGTACGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCAATGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGATGGTGTTCCTGt                                                                                                         >  5:51683/1‑149 (MQ=255)
                                             agcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTAtt                                                                                                                                     <  5:180947/118‑1 (MQ=255)
                                             agcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTAtt                                                                                                                                     >  6:180947/1‑118 (MQ=255)
                                                   tttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGt                                                                                                 <  6:282831/148‑1 (MQ=255)
                                                     tATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTga                                                                                               <  4:308346/148‑1 (MQ=255)
                                                      aTTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGt                                                                                                         >  4:12083/1‑137 (MQ=255)
                                                      aTTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGt                                                                                                         <  3:12083/137‑1 (MQ=255)
                                                        tGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaa                                                                                            >  1:195431/1‑148 (MQ=255)
                                                           tCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTgg                                                                                                                  <  3:28682/123‑1 (MQ=255)
                                                           tCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTgg                                                                                                                  >  4:28682/1‑123 (MQ=255)
                                                           tCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAAtt                                                                                        >  7:323751/1‑149 (MQ=255)
                                                              tGATTATGGATCCGCTCGGAAACCTCCCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCGTGGTGATGCTGGTGTTCGTGTTTGCGGGTGAGAAAATTCTg                                                                                     >  4:279367/1‑149 (MQ=255)
                                                              tGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCTGTACTGATACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGc                                                                                                                     <  2:270556/117‑1 (MQ=255)
                                                               gATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCtt                                                                                                                                        <  6:207202/97‑1 (MQ=255)
                                                               gATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCtt                                                                                                                                        >  5:207202/1‑97 (MQ=255)
                                                                      gATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATg                                                                                                                      >  6:173842/1‑108 (MQ=255)
                                                                       aTCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGTGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGc                                                                                                     >  8:362794/1‑124 (MQ=255)
                                                                       aTCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCtctc                                                                                                                               >  7:65270/1‑98 (MQ=255)
                                                                       aTCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCtctc                                                                                                                               <  8:65270/98‑1 (MQ=255)
                                                                       aTCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGc                                                                                                     <  7:362794/124‑1 (MQ=255)
                                                                        tCCGCTCGGAAACCTACCTCTTTTGATGTGCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGATGCATATTGCTCTCCTGGTGATGCTGCTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           <  3:216719/149‑1 (MQ=255)
                                                                        tCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCt                                                                             >  2:62008/1‑147 (MQ=255)
                                                                        tCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           <  4:116066/149‑1 (MQ=255)
                                                                        tCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           <  1:62008/149‑1 (MQ=255)
                                                                          cGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTgg                                                                                    >  1:346272/1‑138 (MQ=255)
                                                                          cGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTgg                                                                                    <  2:346272/138‑1 (MQ=255)
                                                                               ggAAACCTACCTTTTTTCATGTCCGTACTGAATCATACTGAACCGAAAAGACGGCGGGCTATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTAc                                                                     <  6:51683/148‑1 (MQ=255)
                                                                               ggAAACCTACCTCTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTAc                                                                     <  3:57403/148‑1 (MQ=255)
                                                                                  aaCCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGc                                                                 <  2:195431/149‑1 (MQ=255)
                                                                                   acctacctATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAAACATGGTGCGAGAGATGCTTAATGCTCTCCAGCTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGTCAATTCTTAGCCTACGAg                                                                  >  3:33001/1‑147 (MQ=255)
                                                                                     ctacctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAg                                                               >  3:71648/1‑148 (MQ=255)
                                                                                         ctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGGGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAAcc                                                          >  7:186370/1‑149 (MQ=255)
                                                                                            ttttCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGtc                                                       <  4:71648/149‑1 (MQ=255)
                                                                                            ttttCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGt                                                        <  7:217718/148‑1 (MQ=255)
                                                                                                        tACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTg                                                                                                                   <  5:261626/77‑1 (MQ=255)
                                                                                                        tACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTg                                                                                                                   >  6:261626/1‑77 (MQ=255)
                                                                                                           tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTggcgg                                         >  1:44108/1‑148 (MQ=255)
                                                                                                           tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGATGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           <  8:206919/114‑1 (MQ=255)
                                                                                                           tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGATGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           >  7:206919/1‑114 (MQ=255)
                                                                                                           tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGATGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           <  8:212429/114‑1 (MQ=255)
                                                                                                           tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGATGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                           >  7:212429/1‑114 (MQ=255)
                                                                                                              aaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcat                                      >  6:40281/1‑148 (MQ=255)
                                                                                                                 aTACTGAACCGAAAAGACGGCGGGCAATCATGGTTCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAATTTCTGGCATTTCTTAGCCTACGAGCAGTGACCGTCTCCATTTCTGGCGGCATCAtt                                  >  6:7343/1‑149 (MQ=255)
                                                                                                                     tGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTTCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTTTTTGAGGGTGATAAAATTCTGGCATTTCTTAGCCTACGAGAAGAAACCGTCTCCATTTCTTTCGGCATCATTCTGt                              >  5:139810/1‑149 (MQ=255)
                                                                                                                     tGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGt                              >  1:376222/1‑149 (MQ=255)
                                                                                                                     tGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGt                              >  3:236047/1‑149 (MQ=255)
                                                                                                                       aaCCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCt                                >  8:6013/1‑145 (MQ=255)
                                                                                                                       aaCCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCt                                <  7:6013/145‑1 (MQ=255)
                                                                                                                        aCCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTc                           <  5:40281/149‑1 (MQ=255)
                                                                                                                         ccGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTc                           <  6:15267/148‑1 (MQ=255)
                                                                                                                         ccGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTc                           >  5:15267/1‑148 (MQ=255)
                                                                                                                          cGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCt                          >  8:287725/1‑148 (MQ=255)
                                                                                                                            aaaaGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGAt                       >  3:12283/1‑149 (MQ=255)
                                                                                                                             aaaGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATc                      <  1:138233/149‑1 (MQ=255)
                                                                                                                                 aCGGCGTGCAATCATGGGGCGAGAGTTGCTTATTGCTCTCGTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCa                  <  2:376222/149‑1 (MQ=255)
                                                                                                                                  cggcggGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAt                                                  >  1:81235/1‑116 (MQ=255)
                                                                                                                                  cggcggGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAt                                                  <  2:81235/116‑1 (MQ=255)
                                                                                                                                       ggCAATCATGGTGCGGGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTaaaa            <  8:186370/149‑1 (MQ=255)
                                                                                                                                            tCATGGTGCGAGTGTTGCTTATTGCTCTCCTTGTGATGCTGTTGTTCCTGTTTGCGGGTTAGAAATTTCTGGCATTTCTTAGCCTACGAGCTGAAAGCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGAtt       <  4:33001/149‑1 (MQ=255)
                                                                                                                                            tCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGGCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGAtt       >  1:271797/1‑149 (MQ=255)
                                                                                                                                                 gTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTccc  <  8:323751/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCC  >  NC_000913/3575046‑3575339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: