Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	696,952	T→C	5.2%	intergenic (‑13/+11)	glnU ← / ← leuW	tRNA‑Gln/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,952	0	T	C	5.2%	238.3 / 2.7	77	intergenic (‑13/+11)	glnU/leuW	tRNA‑Gln/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (44/29);  new base C (2/2);  total (46/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696810‑697098                                                                                                                                               |                                                                                                                                                   ttACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGCTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTTGCGATACCCACTCCGTACAACGCTTTCTgg                                                                                                                                              >  7:51120/1‑149 (MQ=255) ttACCGCTTGGCGATACCCCAAAAACAGGGCGGTTAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATACGAACCAGGCACAACCTCGATAAACAGGAGGTGCCTAACCGCTTGGCGATCCCCCATCAGAAGAACGATTTCTgg                                                                                                                                              >  4:107306/1‑149 (MQ=255)  tACCGCTTGGCGATACCCCAATACCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGCACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCACATCCGTACAACGCTTTCTGGt                                                                                                                                             >  3:267917/1‑149 (MQ=255)    ccGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCATGGCGAAACCCCATCCGTACACCGCTTTCTGGTg                                                                                                                                            >  1:244005/1‑148 (MQ=255)                  cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGc                                                                                                                             >  1:110188/1‑149 (MQ=255)                    cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTTTCAAACACCGGTGCCTTACCGCTTGGCGATACCCCCTCCGTACACCGCTGTCTGGTGAATGGTGCGGGAGGCgt                                                                                                                           >  8:139183/1‑148 (MQ=25)                    cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                                                                                                                                             <  2:231949/131‑1 (MQ=255)                    cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                                                                                                                                             >  1:231949/1‑131 (MQ=255)                            gggCGTTGAACCGCTTACTGGAAGAAGATGGCTGGGGTACCTTGATTCGAACCATGGAATGCCGGTATGAAAAACCGTTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGaa                                                                                                                   <  8:51120/149‑1 (MQ=25)                                ggTGAACCGCTTAATCGAAGAAGATGGCGGGGGTACATGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATAACCCTTGCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCg                                                                                                               <  2:110188/149‑1 (MQ=25)                                      ccGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACAcc                                                                                                         >  6:324651/1‑149 (MQ=255)                                              tCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggcg                                                                                                 >  4:149018/1‑149 (MQ=255)                                                gaagaaGATGGGTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGc                                                                                                                                    >  5:260141/1‑112 (MQ=255)                                                gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGc                                                                                                                                    <  6:260141/112‑1 (MQ=255)                                                gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  8:308709/1‑148 (MQ=255)                                                gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  1:348782/1‑148 (MQ=255)                                                gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  8:69077/1‑148 (MQ=255)                                                gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  3:335120/1‑148 (MQ=255)                                                 aagaagATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  7:328810/1‑147 (MQ=255)                                                   gaagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAg                                                                                             >  5:240747/1‑148 (MQ=255)                                                    acgaTGGCGTGGGTACCTGGATTCGAACCAGTGAATGCCTGCATCAAACACCGGTGACTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGaa                                                                                           <  2:244005/147‑1 (MQ=37)                                                    aagaTGGCGTGGGTACCTGTATTCGACCCAGGTAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGaa                                                                                           <  4:267917/149‑1 (MQ=37)                                                      gaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                                                                                                                                                <  5:95890/94‑1 (MQ=255)                                                      gaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                                                                                                                                                >  6:95890/1‑94 (MQ=255)                                                                    cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGt                                                                           >  7:87741/1‑149 (MQ=255)                                                                    cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTCGTGCGt                                                                           >  2:183926/1‑149 (MQ=255)                                                                    cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCCCTTGCCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCTCGACTTGAACTCGCACACCATGCGGCGCCAGAACCTAAATCTGGTGctt                                                                           >  5:279033/1‑147 (MQ=37)                                                                        aTTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGAGCGTCTAc                                                                       >  7:315128/1‑149 (MQ=255)                                                                           cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgc                                                                                                     >  2:387548/1‑116 (MQ=255)                                                                           cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgc                                                                                                     <  1:387548/116‑1 (MQ=255)                                                                            gAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAAt                                                                   >  4:223461/1‑149 (MQ=255)                                                                                cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt                                                                                                                                      <  1:364365/78‑1 (MQ=255)                                                                                cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCg                                                               >  6:33620/1‑149 (MQ=255)                                                                                cAGGGAATGCCGGTATCAAAAACCGGTGCATTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt                                                                                                                                      >  2:364365/1‑78 (MQ=255)                                                                                 aGGGATCGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGc                                                              <  3:181883/149‑1 (MQ=255)                                                                                   ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggc                                                                                                  >  1:222986/1‑111 (MQ=255)                                                                                   ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggc                                                                                                  <  2:222986/111‑1 (MQ=255)                                                                                     aaTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTTGTGCGTCTACCAATTTCGCAACt                                                          >  6:336864/1‑149 (MQ=255)                                                                                     aaTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACt                                                          >  6:342297/1‑149 (MQ=255)                                                                                      aTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTc                                                         <  5:342297/149‑1 (MQ=255)                                                                                      aTGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATATCGCCACTc                                                         <  5:336864/149‑1 (MQ=255)                                                                                          cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCAGGACAACGCTGTCTGGGGAATGGTGCGGGAGGCGAGGCGTGAACTCGCACCCCTAGCGGCGCCAGCACGTAAATCAGCGGCGTCGAGCAAGATAGCCAcatcgg                                                      >  4:181883/1‑143 (MQ=19)                                                                                            gTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCa                                                    >  6:140294/1‑148 (MQ=255)                                                                                               tCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaa                                                 >  4:70499/1‑148 (MQ=255)                                                                                                 aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGAc                                                                                                                        <  4:106352/75‑1 (MQ=255)                                                                                                 aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAACTCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaa                                                >  3:206059/1‑147 (MQ=255)                                                                                                 aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaaaa                                              <  8:328810/149‑1 (MQ=255)                                                                                                 aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaaaa                                              >  1:183026/1‑149 (MQ=255)                                                                                                 aaaaaCCGGTGCCTTACCGCTTGGAGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGAc                                                                                                                        >  3:106352/1‑75 (MQ=255)                                                                                                 aaaaaCCGGTGCCGTACCGCTTGGCGATACCCCATCCTTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACATGAACTCGCACACCTTGCGGCGCCAGAACCTAACTCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaa                                                >  3:281166/1‑147 (MQ=255)                                                                                                  aaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAg                                             <  5:33620/149‑1 (MQ=255)                                                                                                     aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtg                                          <  2:348782/149‑1 (MQ=255)                                                                                                      ccGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtgg                                         <  5:140294/149‑1 (MQ=255)                                                                                                        ggTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtggtg                                       <  6:240747/149‑1 (MQ=255)                                                                                                         gTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtggtgg                                      <  2:183026/149‑1 (MQ=255)                                                                                                                   gCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACgg                             >  1:336531/1‑148 (MQ=255)                                                                                                                          gATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGAGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGaa                     <  8:315128/149‑1 (MQ=255)                                                                                                                           aTACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCACAAAAAAAGATGGTGGCTACGACTGGATTCGAAc                    >  1:338802/1‑149 (MQ=255)                                                                                                                           aTACACCATCCGTACAACGCTTTCTGGTGAATGGTGCGTGAGGCTAGTCTTGAACTCTCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTGCTTCATTAAAAGATGGTGGCTACGACGGGATTCGAAc                    <  2:72288/149‑1 (MQ=255)                                                                                                                              ccccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtg                 <  3:70499/149‑1 (MQ=255)                                                                                                                               cccATCCGTACACCGCTTTCTGGTGAGTGGTGCGGGAGGCGAGCCTTGAACTCGCCCCCATTGCGGCGCCAGACCCTAAATCTGGTGCGTCTACCATTTTCGCCCATCCCGCAAAAAAAGATGGTGCCTCCGCCGGGATTCGACCCtgt                >  4:179677/1‑149 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             >  7:240972/1‑104 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             <  8:240972/104‑1 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             <  4:172784/104‑1 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             >  3:172784/1‑104 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  5:324651/149‑1 (MQ=255)                                                                                                                               cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  8:87741/149‑1 (MQ=255)                                                                                                                                ccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                >  3:221155/1‑148 (MQ=255)                                                                                                                                 cATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAtt                         <  6:116751/138‑1 (MQ=255)                                                                                                                                 cATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAtt                         >  5:116751/1‑138 (MQ=255)                                                                                                                                  aTCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAc             <  3:107306/149‑1 (MQ=255)                                                                                                                                   tCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAcc            <  7:69077/149‑1 (MQ=255)                                                                                                                                     cGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAccc           >  7:38363/1‑148 (MQ=255)                                                                                                                                         cAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCcatca      >  8:306030/1‑149 (MQ=255)                                                                                                                                          aaCGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCcatca      >  1:195701/1‑148 (MQ=255)                                                                                                                                             gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTGGAACCTGTGACCCCATCATTAt  <  2:336531/149‑1 (MQ=255)                                                                                                                                             gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCGACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAGTCGAACCTGTGACCCCATCATTAt  >  2:260715/1‑149 (MQ=255)                                                                                                                                               tttCTGTTGAATTGTGCGGGAGGCGAGTCTTCTTGTCGCAGACCTTGCGGCGCAAGATCCTAAATCTGGTGCGTCTTCCTATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACTACGGGATTCGATCGTGTGTCGCCATCATTAt  <  6:101717/147‑1 (MQ=255)                                                                                                                                               tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                >  8:240914/1‑133 (MQ=255)                                                                                                                                               tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  7:240914/133‑1 (MQ=255)                                                                                                                                               tttCTGGTGAATGGTGAGGGAGGCGAGACTTGAACTCGCACACCTTGCGTCGCCAGAACCTAAATCTGGTGCTTCTACCAATTTCGCCACTCCCGCTAAAAAAGATGCTGGCTACGACGGGATTCGAACCTGTGACCCAATCAatat  >  5:101717/1‑147 (MQ=255)                                                                                                                                               |                                                                                                                                                   TTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696810‑697098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑