Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	810,502	A→G	6.1%	Y41C (TAT→TGT)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	810,502	0	A	G	6.1%	200.2 / 2.8	65	Y41C (TAT→TGT)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base G (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAG  >  NC_000913/810354‑810641                                                                                                                                                     |                                                                                                                                            cgacgaATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTa                                                                                                                                             >  6:257873/1‑149 (MQ=255) cgacgaATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTa                                                                                                                                             >  5:284798/1‑149 (MQ=255)       atatTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGaa                                                                                                                                       >  4:81413/1‑149 (MQ=255)       atatTACAACGCGGCAGCATTATGAGCCGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGaa                                                                                                                                       >  3:185313/1‑149 (MQ=255)          ttACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACtt                                                                                                                                    >  6:133202/1‑149 (MQ=255)             cAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTc                                                                                                                                 <  6:148608/149‑1 (MQ=255)               aCGCGGCAGCCTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTc                                                                                                                                 >  4:104601/1‑147 (MQ=255)               aCGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCa                                                                                                                               <  3:81413/149‑1 (MQ=255)                   ggCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTaa                                                                                                                           >  8:143458/1‑149 (MQ=255)                       gcaTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACttt                                                                                                                                   >  5:18771/1‑137 (MQ=255)                       gcaTTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACttt                                                                                                                                   <  6:18771/137‑1 (MQ=255)                               aGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAgg                                                                                                               <  5:280401/149‑1 (MQ=255)                               aGCCGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAgg                                                                                                               <  4:185313/149‑1 (MQ=255)                                 cTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGCCGCTGGCTGGTGGCGGATGCTCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGtt                                                                                                             >  7:218112/1‑149 (MQ=255)                                    gCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGttt                                                                                                            >  1:81810/1‑147 (MQ=255)                                    gCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTaa                                                                                                          <  8:218112/149‑1 (MQ=255)                                      aGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCt                                                                                                                                          <  2:183135/115‑1 (MQ=255)                                      aGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCt                                                                                                                                          >  1:183135/1‑115 (MQ=255)                                        gagaAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCca                                                                                                      <  5:257873/149‑1 (MQ=255)                                          gaAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCcat                                                                                                     >  7:380160/1‑148 (MQ=255)                                           aaaaTCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCcatca                                                                                                   >  7:131766/1‑149 (MQ=255)                                                 aaCGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGaa                                                                                                                                       <  2:283133/107‑1 (MQ=255)                                                   cgcgGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCaaa                                                                                           <  8:77252/149‑1 (MQ=255)                                                        cgcTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGAAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAATCCATCATCCGCAAATTATc                                                                                      <  2:51369/149‑1 (MQ=255)                                                         gcTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGAATATTTAGGTTTAAGCCATCATCCGCAAATTATcc                                                                                     <  3:188922/149‑1 (MQ=255)                                                           tCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATcc                                                                                     <  4:235483/147‑1 (MQ=255)                                                                 cgcgGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTgg                                                                             <  6:284798/149‑1 (MQ=255)                                                                           tgcCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTc                                                                                                                                 >  3:187211/1‑87 (MQ=255)                                                                           tgcCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTc                                                                                                                                 <  4:187211/87‑1 (MQ=255)                                                                             ccGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCg                                                                 >  2:130873/1‑149 (MQ=255)                                                                              cGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCg                                                                 >  8:247749/1‑148 (MQ=255)                                                                               gATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCaaa                                                                                           >  7:197157/1‑121 (MQ=255)                                                                               gATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCaaa                                                                                           <  8:197157/121‑1 (MQ=255)                                                                                  gCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGa                                                               <  2:321441/146‑1 (MQ=255)                                                                                  gCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGa                                                               >  1:321441/1‑146 (MQ=255)                                                                                    ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTACCGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAAt                                                          >  5:82000/1‑149 (MQ=255)                                                                                    ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAggggg                                                                   <  1:171880/140‑1 (MQ=255)                                                                                    ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAggggg                                                                   >  2:171880/1‑140 (MQ=255)                                                                                    ccTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAAt                                                          >  4:129940/1‑149 (MQ=255)                                                                                         gTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTgg                                                      >  4:93583/1‑148 (MQ=255)                                                                                            gCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCAt                                                   <  8:131766/148‑1 (MQ=255)                                                                                            gCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATc                                                  >  1:297869/1‑149 (MQ=255)                                                                                              cGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGAAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCg                                                                 <  1:86440/132‑1 (MQ=255)                                                                                              cGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGAAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCg                                                                 >  2:86440/1‑132 (MQ=255)                                                                                                ttATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTa                                              <  1:130873/149‑1 (MQ=255)                                                                                                          gcgcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTc                                    <  3:129940/149‑1 (MQ=255)                                                                                                            gcAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCg                                  >  8:194589/1‑149 (MQ=255)                                                                                                              aaGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGt                                >  1:288085/1‑149 (MQ=255)                                                                                                               aGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTc                               >  3:108065/1‑149 (MQ=255)                                                                                                                ggAGACGGACGCTGGCTGGTGGCGGATGAACGCCAGTATCTGAACTTTTCCAGTAACGAATATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCATGGCAGCAGGGGGCGGAGAAATTTGGCATCGGTAGCGGCGGCTCCGGtga                              >  4:322844/1‑147 (MQ=255)                                                                                                                 gAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCAc                             <  8:380160/149‑1 (MQ=255)                                                                                                                      ggACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACGTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAg                        >  7:3005/1‑149 (MQ=255)                                                                                                                        aCGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCg                      <  7:194589/149‑1 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTGTCTGAACTTTTCCAGTAACGATTATTTAGGTTTAATCCATCATCCGCAAATTATCCGTGCCTGGcagcag                                                                       <  7:114398/96‑1 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTGTCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGcagcag                                                                       <  7:119942/96‑1 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTGTCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGcagcag                                                                       >  8:114398/1‑96 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTGTCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGcagcag                                                                       >  8:119942/1‑96 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTta                  >  1:355762/1‑149 (MQ=255)                                                                                                                            tggctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTta                  >  8:92975/1‑149 (MQ=255)                                                                                                                              gctggTTGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGATTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGGCAGCGGTtat                 <  2:280118/148‑1 (MQ=255)                                                                                                                              gctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTtata                <  5:133202/149‑1 (MQ=255)                                                                                                                              gctggTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTtata                <  5:192548/149‑1 (MQ=255)                                                                                                                                  gtggCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGc              <  7:143458/147‑1 (MQ=255)                                                                                                                                        gatgatCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCa      >  3:36502/1‑149 (MQ=255)                                                                                                                                          tgatCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATc    <  3:104601/149‑1 (MQ=255)                                                                                                                                          tgatCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATc    <  3:93583/149‑1 (MQ=255)                                                                                                                                            atCGCCAGTATCTGAACTTTTCCAGTTACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGTAGCAATTTGGCTTCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAg  <  3:322844/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            CGACGAATATTACAACGCGGCAGCATTATGAGCTGGCAGGAGAAAATCAACGCGGCGCTCGATGCGCGGCGTGCTGCCGATGCCCTGCGTCGCCGTTATCCGGTGGCGCAAGGAGCCGGACGCTGGCTGGTGGCGGATGATCGCCAGTATCTGAACTTTTCCAGTAACGATTATTTAGGTTTAAGCCATCATCCGCAAATTATCCGTGCCTGGCAGCAGGGGGCGGAGCAATTTGGCATCGGTAGCGGCGGCTCCGGTCACGTCAGCGGTTATAGCGTGGTGCATCAG  >  NC_000913/810354‑810641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑