mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L001_R1_001.good.fq | 368,020 | 52,652,105 | 100.0% | 143.1 bases | 149 bases | 96.7% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L001_R2_001.good.fq | 368,020 | 52,312,278 | 100.0% | 142.1 bases | 149 bases | 97.5% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L002_R1_001.good.fq | 334,937 | 47,924,068 | 100.0% | 143.1 bases | 149 bases | 96.8% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L002_R2_001.good.fq | 334,937 | 47,636,912 | 100.0% | 142.2 bases | 149 bases | 97.3% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L003_R1_001.good.fq | 357,539 | 51,136,755 | 100.0% | 143.0 bases | 149 bases | 96.6% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L003_R2_001.good.fq | 357,539 | 50,803,135 | 100.0% | 142.1 bases | 149 bases | 97.3% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L004_R1_001.good.fq | 356,609 | 50,972,600 | 100.0% | 142.9 bases | 149 bases | 96.9% |
| errors | Plate-1-BOP-27-ACEF-END-4_S4_L004_R2_001.good.fq | 356,609 | 50,657,806 | 100.0% | 142.1 bases | 149 bases | 97.5% |
| total | 2,834,210 | 404,095,659 | 100.0% | 142.6 bases | 149 bases | 97.1% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 78.8 | 3.7 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 18003 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 55 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.004 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.77410 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 01:13:10 12 Sep 2020 | 01:13:54 12 Sep 2020 | 44 seconds |
| Read alignment to reference genome | 01:13:54 12 Sep 2020 | 01:19:20 12 Sep 2020 | 5 minutes 26 seconds |
| Preprocessing alignments for candidate junction identification | 01:19:20 12 Sep 2020 | 01:20:03 12 Sep 2020 | 43 seconds |
| Preliminary analysis of coverage distribution | 01:20:03 12 Sep 2020 | 01:21:41 12 Sep 2020 | 1 minute 38 seconds |
| Identifying junction candidates | 01:21:41 12 Sep 2020 | 01:21:58 12 Sep 2020 | 17 seconds |
| Re-alignment to junction candidates | 01:21:58 12 Sep 2020 | 01:23:34 12 Sep 2020 | 1 minute 36 seconds |
| Resolving best read alignments | 01:23:34 12 Sep 2020 | 01:24:41 12 Sep 2020 | 1 minute 7 seconds |
| Creating BAM files | 01:24:41 12 Sep 2020 | 01:26:11 12 Sep 2020 | 1 minute 30 seconds |
| Tabulating error counts | 01:26:11 12 Sep 2020 | 01:26:42 12 Sep 2020 | 31 seconds |
| Re-calibrating base error rates | 01:26:42 12 Sep 2020 | 01:26:44 12 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 01:26:44 12 Sep 2020 | 01:32:57 12 Sep 2020 | 6 minutes 13 seconds |
| Polymorphism statistics | 01:32:57 12 Sep 2020 | 01:32:58 12 Sep 2020 | 1 second |
| Output | 01:32:58 12 Sep 2020 | 01:33:13 12 Sep 2020 | 15 seconds |
| Total | 20 minutes 3 seconds | ||
