Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	756,291	Δ11 bp	18.8%	coding (385‑395/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 756290	72 (0.820)	16 (0.190)	15/246	NT	18.8%	coding (384/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
	?	NC_000913	756302 =	70 (0.840)					coding (396/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit

CTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/756137‑756290 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  >  NC_000913/756302‑756447                                                                                                                                                                                                                                                                                                              CTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCAGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCG                                                                                                                                                         <  4:240563/149‑1    TATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTCGGCG                                                                                                                                                      <  3:227367/149‑1             CCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACT                                                                                                                                             <  4:222420/149‑1                   CGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCA                                                                                                                                       <  7:312553/149‑1                                 TGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTAC                                                                                                                         <  8:402280/149‑1                                                cgcAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTT                                                                                                          <  2:228074/146‑1                                                CGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTT                                                                                                          >  6:246268/1‑149                                                                ACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTT                                                                                                <  1:302551/143‑1                                                                ACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTT                                                                                                >  2:302551/1‑143                                                                                CTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACC                                                                                                                        >  7:155951/1‑103                                                                                CTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACC                                                                                                                        <  8:155951/103‑1                                                                                                                        GTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGT                                  >  1:392340/1‑149                                                                                                                        GTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGT                                  >  1:350922/1‑149                                                                                                                         TCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTG                                 <  2:350922/149‑1                                                                                                                                 GCGGTCAGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCA                         <  2:392340/149‑1                                                                                                                                       AGTCGAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCAAACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGG                   >  4:330623/1‑149                                                                                                                                           GAAAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACCCCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCG               >  1:120138/1‑149                                                                                                                                             AAAACTTCGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGT             <  2:120138/149‑1                                                                                                                                                     GGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAGAACCAGGATGGCGCGGTGGTGGGTT     >  8:268866/1‑149                                                                                                                                                     GGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGC                                                                                                        >  2:30656/1‑50                                                                                                                                                     GGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGC                                                                                                        <  1:30656/50‑1                                                                                                                                                       CGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTG    >  2:278602/1‑148                                                                                                                                                        GGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTA  >  5:152744/1‑149                                                                                                                                                        GGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  <  1:56747/149‑1                                                                                                                                                        GGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  <  5:246268/149‑1                                                                                                                                                                                                                                                                                                              CTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/756137‑756290 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  >  NC_000913/756302‑756447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.