New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4193534 =93 (1.060)3 (0.040) 3/246 NT 3.2% coding (43/756 nt) thiF adenylyltransferase, modifies ThiS C‑terminus
?NC_000913 4193590 = 91 (1.090)coding (615/636 nt) thiE thiamine phosphate synthase (thiamine phosphate pyrophosphorylase)
Rejected: Frequency below/above cutoff threshold.

CTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4193687‑4193534
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcctgcAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGC  >  NC_000913/4193590‑4193736
                                                                                                                                                                                                                                                                                                             
CTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAAT                                                                                                                                                          >  5:82927/1‑149
CTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAAT                                                                                                                                                          >  8:344576/1‑149
                                                    TCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGC                                                                                                                                                     <  1:78141/102‑1
                                                    TCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGC                                                                                                                                                     >  2:78141/1‑102
                                                                                          TGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGTGGCTTGAGTAATGGCGCTGACGACGGCGATACT                                                                             <  3:82317/136‑1
                                                                                          TGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGTGGCTTGAGTAATGGCGCTGACGACGGCGATACT                                                                             >  4:82317/1‑136
                                                                                                   CAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACC                                                       >  8:307637/1‑149
                                                                                                                                                         CAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGC  >  7:98230/1‑148
                                                                                                                                                         CAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGA                                                                                          >  3:123293/1‑60
                                                                                                                                                         CAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGA                                                                                          <  4:123293/60‑1
                                                                                                                                                                                                                                                                                                             
CTGCGGTGATAGCAACGGGTGTCGGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAATCCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4193687‑4193534
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcctgcAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGC  >  NC_000913/4193590‑4193736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.