Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,216,776 C→T 5.2% G211D (GGT→GAT)  yehX ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,7760CT 5.2% 252.7 / 3.0 77G211D (GGT→GAT) yehXputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (33/40);  new base T (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

GCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTC  >  NC_000913/2216629‑2216912
                                                                                                                                                   |                                                                                                                                        
gCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCAcc                                                                                                                                         >  7:148517/1‑149 (MQ=255)
gCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCAATCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCAcc                                                                                                                                         >  8:87111/1‑149 (MQ=255)
    gTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTg                                                                                                                                      <  8:290490/148‑1 (MQ=255)
     ttcttcGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGCACAAACTCATTCGCCGGACGAGTCAGCAGCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGAt                                                                                                                                    <  2:267363/149‑1 (MQ=255)
        ttcGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCa                                                                                                                                 >  6:40934/1‑149 (MQ=255)
          cgcgACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATcc                                                                                                                                  <  4:213784/146‑1 (MQ=255)
          cgcgACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCa                                                                                                                                 <  3:106933/147‑1 (MQ=255)
              aCGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATa                                                                                                                           <  7:87111/149‑1 (MQ=255)
               cGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATAc                                                                                                                          <  3:380400/149‑1 (MQ=255)
               cGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATAc                                                                                                                          <  8:272424/149‑1 (MQ=255)
               cGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCAAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATAc                                                                                                                          <  5:15357/149‑1 (MQ=255)
                 cACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCa                                                                                                                        <  4:308427/149‑1 (MQ=255)
                  aCGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATAc                                                                                                                          >  5:369613/1‑146 (MQ=255)
                   cGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACGCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCCGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCATCGTGATCCATCAATAc                                                                                                                          >  5:147396/1‑145 (MQ=255)
                   cGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACGCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCATCGTGATCCATCAATAc                                                                                                                          >  5:153273/1‑145 (MQ=255)
                    gTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGAt                                                                                                                     >  3:277739/1‑149 (MQ=255)
                    gTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACAGTGATCCATCAATACCAGAt                                                                                                                     >  6:280314/1‑149 (MQ=255)
                        tCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCa                                                                                                                        >  7:21309/1‑142 (MQ=255)
                        tCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCa                                                                                                                        <  8:21309/142‑1 (MQ=255)
                        tCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTc                                                                                                                 <  2:148932/149‑1 (MQ=255)
                          cGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTg                                                                                                               <  3:155190/149‑1 (MQ=255)
                                  tACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCg                                                                                                        >  1:354719/1‑148 (MQ=255)
                                   aCGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTa                                                                                                      <  5:40934/149‑1 (MQ=255)
                                          gAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCtc                                                                                               <  8:148517/149‑1 (MQ=255)
                                          gAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCt                                                                                                >  2:90554/1‑148 (MQ=255)
                                          gAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCt                                                                                                >  7:182903/1‑148 (MQ=255)
                                           aatgCAGGCCCACACCCAGTTCACTACGTTCAAAAAACTGGCCGACAATATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCtca                                                                                              <  8:182903/146‑1 (MQ=255)
                                           aaaGGAGGCGCACACCGAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCAATCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGGGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCtca                                                                                              <  3:157533/149‑1 (MQ=255)
                                           aaaGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCtca                                                                                              <  6:369613/149‑1 (MQ=255)
                                                 ggCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGAGTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAata                                                                                        <  5:118982/149‑1 (MQ=255)
                                                    gcACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCa                                                                                     >  3:185197/1‑149 (MQ=255)
                                                     cacaCCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCAt                                                                                    <  1:90554/149‑1 (MQ=255)
                                                     cacaCCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCAt                                                                                    <  8:120654/149‑1 (MQ=255)
                                                       cacCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTTATCCATCAATACCATATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGa                                                                                  <  8:381364/149‑1 (MQ=255)
                                                        acCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATc                                                                                      <  6:239839/144‑1 (MQ=255)
                                                        acCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATc                                                                                      >  5:239839/1‑144 (MQ=255)
                                                          ccAGTTCACTACGTCCAAAAATCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGt                                                                                >  6:108380/1‑148 (MQ=255)
                                                            aGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGAc                                                                             <  3:172643/149‑1 (MQ=255)
                                                                   tACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGAc                                                                             <  3:84885/142‑1 (MQ=255)
                                                                   tACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGAc                                                                             >  4:84885/1‑142 (MQ=255)
                                                                         cAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATcc                                                                                                                                  <  5:175365/83‑1 (MQ=255)
                                                                         cAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATcc                                                                                                                                  >  6:175365/1‑83 (MQ=255)
                                                                          aaaaaaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGca                                                                        >  1:333126/1‑140 (MQ=255)
                                                                          aaaaaaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGca                                                                        <  2:333126/140‑1 (MQ=255)
                                                                           aaaaaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCACTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTc                                                                                                                 <  6:149144/98‑1 (MQ=255)
                                                                            aaaaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGa                                                                                                                      >  8:259001/1‑92 (MQ=255)
                                                                            aaaaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGa                                                                                                                      <  7:259001/92‑1 (MQ=255)
                                                                              aaCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGccc                                                           <  1:11944/149‑1 (MQ=255)
                                                                               aCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCa                                                                           <  8:235056/132‑1 (MQ=255)
                                                                               aCTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCa                                                                           >  7:235056/1‑132 (MQ=255)
                                                                                cTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCa                                                         <  4:33748/149‑1 (MQ=255)
                                                                                 tGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAg                                                        >  2:286626/1‑149 (MQ=255)
                                                                                   gCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGc                                                       >  3:329330/1‑148 (MQ=255)
                                                                                          aaaTCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGt                                                >  1:104917/1‑148 (MQ=255)
                                                                                           aaTCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGt                                                >  1:21684/1‑147 (MQ=255)
                                                                                           aaTCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGt                                                <  2:21684/147‑1 (MQ=255)
                                                                                              cATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGa                                              >  2:414351/1‑146 (MQ=255)
                                                                                              cATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGa                                              <  1:414351/146‑1 (MQ=255)
                                                                                                  cGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCATCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATgcgc                                        <  6:153273/148‑1 (MQ=255)
                                                                                                  cGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCATCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATgcgc                                        <  6:147396/148‑1 (MQ=255)
                                                                                                               aGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCaaa                                                    >  3:252964/1‑123 (MQ=255)
                                                                                                               aGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCaaa                                                    <  4:252964/123‑1 (MQ=255)
                                                                                                               aGCATCGTCAGCGGATTGCCCTGCTGCACAACTTCACCGAGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCAAAATGGTACGCCACAGCAAACGGAGAATGCGCGTCATCTCttgttg                          >  2:138182/1‑149 (MQ=255)
                                                                                                                   tCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAAc                      <  2:104917/149‑1 (MQ=255)
                                                                                                                    cGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGa                                              <  4:189917/124‑1 (MQ=255)
                                                                                                                    cGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGa                                              >  3:189917/1‑124 (MQ=255)
                                                                                                                          cGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCTAACGGTGAATGCGCGTCATCTCTTGTTGCAAcgcgccgc               <  4:329330/149‑1 (MQ=255)
                                                                                                                            gATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAAcgcgccgcgc             >  2:405908/1‑149 (MQ=255)
                                                                                                                             aTTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCcgcgcg            >  1:167303/1‑149 (MQ=255)
                                                                                                                                 cccTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGAcc                                                                            <  7:278009/81‑1 (MQ=255)
                                                                                                                                 cccTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGAcc                                                                            >  8:278009/1‑81 (MQ=255)
                                                                                                                                    tcctgcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGCTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCgg     >  8:343911/3‑149 (MQ=255)
                                                                                                                                      ctgcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCgg     <  5:108380/147‑1 (MQ=255)
                                                                                                                                        gcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  >  2:289202/1‑148 (MQ=255)
                                                                                                                                        gcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  >  8:398037/1‑148 (MQ=255)
                                                                                                                                         cACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  >  2:405292/1‑147 (MQ=255)
                                                                                                                                         cACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  >  6:212283/1‑147 (MQ=255)
                                                                                                                                         cACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  <  5:252440/147‑1 (MQ=255)
                                                                                                                                         cACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTc  >  5:171517/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
GCTCGTTCTTCGCGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTACGTCCAAAAAACTGGCGGACAAAATCATTCGCCGGACGAGTCAGCATCGTCAGCGGATTGCCCTGCTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTC  >  NC_000913/2216629‑2216912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: