Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,357,653 A→G 7.0% L250L (CTA→CTG yfaD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,357,6530AG 7.0% 258.9 / 6.8 86L250L (CTA→CTGyfaDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base A (40/40);  new base G (2/4);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGT  >  NC_000913/2357515‑2357797
                                                                                                                                          |                                                                                                                                                
tAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGAGATAACTCCCGTTTCACCCCTTATATTCAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGTGATTAAAGAGTAGTGCTGAACGGCGACGGCAGGAAg                                                                                                                                        >  2:174584/1‑149 (MQ=255)
tAATGACAGCCAGCTACAAACACTGTGTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGCCTACGGCAGGAAg                                                                                                                                        >  1:207845/1‑149 (MQ=255)
  aTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAggg                                                                                                                                      <  7:81117/149‑1 (MQ=255)
  aTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAgg                                                                                                                                       <  8:369669/148‑1 (MQ=255)
  aTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAg                                                                                                                                        <  7:225342/147‑1 (MQ=255)
  aTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAg                                                                                                                                        <  7:29132/147‑1 (MQ=255)
   tGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAggg                                                                                                                                      >  8:385464/1‑148 (MQ=255)
     aaatccagcTACAAAAACTTTTTAATTATATGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTTCCGAACGTTCACCACTACAAAATGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCAt                                                                                                                                   <  6:344308/145‑1 (MQ=38)
      cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATc                                                                                                                                  <  3:15388/149‑1 (MQ=255)
       agccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATc                                                                                                                                  >  7:163416/1‑148 (MQ=255)
         ccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCaaa                                                                                                                               >  3:113344/1‑149 (MQ=255)
             cTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtgg                                                                                                                           <  1:229359/149‑1 (MQ=255)
              tACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggc                                                                                                                          >  2:120952/1‑149 (MQ=255)
                 aaaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctgg                                                                                                                       >  7:252748/1‑149 (MQ=255)
                  aaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtgggtgg                                                                                                                       >  6:393223/1‑148 (MQ=255)
                       tGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAgg                                                                                                                   >  8:195357/1‑147 (MQ=255)
                         tttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAg                                                                                                                <  7:4837/148‑1 (MQ=255)
                         tttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAg                                                                                                                <  6:2290/148‑1 (MQ=255)
                         tttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGATGAACGGCTACGGCAGGAAGGGCAGCAAGTTGGCTGGaaggaagg                                                                                                               >  6:284618/1‑149 (MQ=255)
                         tttAATTATCTGCGGCAATGCGGCGATACCTCCCGTTGCACCCGTTTTCTTGAGGAGATTGACTAACGTTCACGACTACAAAAGGAGAGATTAATGACTATTGCTGAACGCCTGCGGCAGGAAGGTCATCAAATTGGCTGGCAGGAAg                                                                                                                <  2:207845/148‑1 (MQ=25)
                           tAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                              <  8:42598/148‑1 (MQ=255)
                             aTTATCTGCTGCAATTCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCAAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATg                                                                                                           <  3:57710/149‑1 (MQ=255)
                             aTTATCTGCTGCAAAGCGGCGATACCTCCAGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGACCGGCTACGGCAGGAAGGGCATGAAATTGGCTGGCAGGAAGGTAt                                                                                                            <  1:174584/148‑1 (MQ=255)
                              ttATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTaaat                                                                                                          >  5:24317/1‑146 (MQ=255)
                                 tCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGACTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGTAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt                                                                                                        >  6:96837/1‑148 (MQ=255)
                                     ctgcAATGCGGCGATACCTCCGGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAAc                                                                                                    >  4:193334/1‑148 (MQ=255)
                                        cactGCGGCGATACCTCCCGTTTCACACGGGTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGAATAATGTCTATTGCTGAACGGCTACGGCAGGAAGGTCATCAAATTGGCTGGCAGGAAGGTATGCATGAACaa                                                                                                  <  4:302257/144‑1 (MQ=25)
                                        cAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGc                                                                                                <  1:369340/149‑1 (MQ=255)
                                         aaTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCCCTACAAAAGGAGAGATTAATGACTATTACTCAAAGGCTACGGCAGGAAGGGCATCAAATTGGATGCCAGGAAGGTATGCATCAACAAGc                                                                                                >  5:379460/1‑148 (MQ=37)
                                                  aTACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAAtt                                                                                      >  7:358250/1‑149 (MQ=255)
                                                  aTACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAAtt                                                                                      >  6:295340/1‑149 (MQ=255)
                                                   tACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc                                                                                                          >  2:410438/1‑128 (MQ=255)
                                                   tACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTg                                                                                     <  7:385464/149‑1 (MQ=255)
                                                      cTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAgg                                                                                                                                       >  7:213592/1‑96 (MQ=255)
                                                      cTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAgg                                                                                                                                       <  8:213592/96‑1 (MQ=255)
                                                       tCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCttt                                                                                 >  8:270014/1‑149 (MQ=255)
                                                         ccGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTg                                                                                <  8:252748/148‑1 (MQ=255)
                                                           gTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTgcgc                                                                             <  1:120952/149‑1 (MQ=255)
                                                               cACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAgga                                                                                                                                      <  8:340738/88‑2 (MQ=255)
                                                               cACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAgga                                                                                                                                      >  7:340738/1‑87 (MQ=255)
                                                                aCCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt                                                                        >  6:237878/1‑149 (MQ=255)
                                                                aCCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt                                                                        <  7:252623/149‑1 (MQ=255)
                                                                aCCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt                                                                        >  2:390859/1‑149 (MQ=255)
                                                                 cccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTg                                                                       >  5:357656/1‑149 (MQ=255)
                                                                   cGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGGATGAACAATGCATTTAAATTGCTTTGAGAATTCTg                                                                       >  1:409677/1‑147 (MQ=25)
                                                                     ttttATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCa                                                                                              >  4:57405/1‑122 (MQ=255)
                                                                     ttttATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCa                                                                                              <  3:57405/122‑1 (MQ=255)
                                                                       ttATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCAGCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCa                                                                  <  5:284618/148‑1 (MQ=255)
                                                                        tATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAg                                                                 <  7:195357/148‑1 (MQ=255)
                                                                         aTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAgg                                                                <  4:113344/148‑1 (MQ=255)
                                                                          ttGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                              >  8:392875/1‑101 (MQ=255)
                                                                          ttGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGTAGCAGGGc                                                              <  8:123311/149‑1 (MQ=255)
                                                                            gaggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCt                                                             <  5:237878/148‑1 (MQ=255)
                                                                             aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGTCTACGGAAGGAAGGGAATCAAATTGGCTGGAAGGAAGGTATGCATGAACAAGCAATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCttt                                                           >  4:151378/1‑149 (MQ=37)
                                                                             aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCttt                                                           >  7:94161/1‑149 (MQ=255)
                                                                             aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCttt                                                           <  8:67156/149‑1 (MQ=255)
                                                                                      ccGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTga                                                   <  8:163416/148‑1 (MQ=255)
                                                                                      ccGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTga                                                   <  8:94161/148‑1 (MQ=255)
                                                                                      ccGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAATTGCTTTGAGCATGCTGGAGCAGGGCTTTGAACGTga                                                   >  3:364975/1‑148 (MQ=255)
                                                                                        gAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAt                                                                                                            <  5:311184/89‑1 (MQ=255)
                                                                                        gAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAt                                                                                                            >  6:311184/1‑89 (MQ=255)
                                                                                        gAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGAt                                                >  6:278328/1‑149 (MQ=255)
                                                                                            gTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATtgtg                                            <  5:278328/149‑1 (MQ=255)
                                                                                              tCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtggctg                                                                                                                        <  7:10716/71‑1 (MQ=255)
                                                                                              tCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATtggctg                                                                                                                        >  8:10716/1‑71 (MQ=255)
                                                                                                      aCAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACaa                                  <  2:180800/149‑1 (MQ=255)
                                                                                                        aaaaGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAAcc                                >  1:300819/1‑149 (MQ=255)
                                                                                                            ggAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTg                                         >  7:353308/1‑136 (MQ=255)
                                                                                                            ggAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTg                                         <  8:353308/136‑1 (MQ=255)
                                                                                                               gagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCa                         >  8:231095/1‑149 (MQ=255)
                                                                                                                agaTTAATGACTATTGCTGAACGGCTACGGCAGGTAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCAc                        <  5:96837/149‑1 (MQ=255)
                                                                                                                     aaTGACTATTGCTGAACGGCTACGGCAGGAAGGGCAACAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATg                   >  7:324718/1‑149 (MQ=255)
                                                                                                                      aTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGc                  >  1:230723/1‑149 (MQ=255)
                                                                                                                      aTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGc                  >  8:156684/1‑149 (MQ=255)
                                                                                                                       tGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGGAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTgaga                                                 >  1:319618/1‑117 (MQ=255)
                                                                                                                       tGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGGAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTgaga                                                 <  2:319618/117‑1 (MQ=255)
                                                                                                                       tGACTATTGCGGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCt                 <  3:193334/149‑1 (MQ=255)
                                                                                                                         aCTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGa               <  1:390859/149‑1 (MQ=255)
                                                                                                                           tATTGCTGAACGGCTACGGCAAGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGata             >  1:41237/1‑149 (MQ=255)
                                                                                                                             ttGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGACTGTGCTGGCGACAACCCAACTCACTGATGCTGATAtt           <  8:358250/149‑1 (MQ=255)
                                                                                                                                cTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTcc         <  7:231095/148‑1 (MQ=255)
                                                                                                                                 tGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCg        >  2:227325/1‑148 (MQ=255)
                                                                                                                                  gAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa      >  3:156730/1‑149 (MQ=255)
                                                                                                                                   aaCGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa      <  7:270014/148‑1 (MQ=255)
                                                                                                                                   aaCGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa      <  7:156684/148‑1 (MQ=255)
                                                                                                                                      ggCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGt  <  2:300819/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGT  >  NC_000913/2357515‑2357797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: