Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,296 T→G 6.7% intergenic (‑176/+89) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,2960TG 6.7% 310.0 / 11.8 102intergenic (‑176/+89)yfcJ/fabBputative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base T (52/43);  new base G (3/4);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 104 total aligned reads displayed.

AGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440149‑2440444
                                                                                                                                                   |                                                                                                                                                    
aggagTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATc                                                                                                                                                     <  6:296066/149‑1 (MQ=255)
  gagTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAg                                                                                                                                                   <  1:18975/149‑1 (MQ=255)
   agTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAgg                                                                                                                                                  <  4:366247/149‑1 (MQ=255)
          ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtt                                                                                                                                           <  6:84683/149‑1 (MQ=255)
           cGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttt                                                                                                                                          <  3:256468/149‑1 (MQ=255)
            ggctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTGTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         <  3:290852/147‑1 (MQ=255)
                  tctctcTGGTTTCAGGGTTAAGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                   >  2:282446/1‑149 (MQ=255)
                      tctGGTTTGAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGc                                                                                                                                       <  8:2495/141‑1 (MQ=255)
                      tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGc                                                                                                                                       >  7:2495/1‑141 (MQ=255)
                      tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                   <  1:312340/145‑1 (MQ=255)
                            ttCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTGAGTGCcggcgg                                                                                                                         >  4:120020/1‑149 (MQ=255)
                               aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttttcacat                                                                                                                                   <  4:359119/136‑7 (MQ=255)
                               aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttttcacat                                                                                                                                   >  3:359119/1‑130 (MQ=255)
                                  gTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAt                                                                                                                   <  8:342144/149‑1 (MQ=255)
                                  gTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAt                                                                                                                   <  8:360388/149‑1 (MQ=255)
                                        gTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcgc                                                                                                             >  4:267235/1‑149 (MQ=255)
                                         tGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGcgcg                                                                                                            >  6:243203/1‑149 (MQ=255)
                                                gCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  4:24188/149‑1 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         >  6:191916/1‑112 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         <  5:191916/112‑1 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  8:105517/148‑1 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     >  7:105517/1‑148 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  7:310398/1‑149 (MQ=255)
                                                 cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  2:134718/1‑149 (MQ=255)
                                                  aGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  1:37685/1‑148 (MQ=255)
                                                    gATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGAt                                                                                                 <  1:401223/149‑1 (MQ=255)
                                                      tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGCGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               >  1:389977/1‑149 (MQ=255)
                                                      tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               >  2:108243/1‑149 (MQ=255)
                                                      tttAAAGCGTTCTTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               <  5:332010/149‑1 (MQ=255)
                                                          aCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACg                                                                                           >  2:241096/1‑149 (MQ=255)
                                                           cgcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGACAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGc                                                                                          >  2:212823/1‑149 (MQ=255)
                                                            gcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         >  7:164984/1‑149 (MQ=255)
                                                               tACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGgc                                                                                      >  5:234500/1‑149 (MQ=255)
                                                                  gTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGcgcg                                                                                   >  2:136288/1‑149 (MQ=255)
                                                                   tCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  >  3:106091/1‑149 (MQ=255)
                                                                    cTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTc                                                                                 >  2:241604/1‑149 (MQ=255)
                                                                     ttttCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTc                                                                                 >  3:158995/1‑148 (MQ=255)
                                                                        tCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCTCGTAACGTCGGATGCGCCGCTGGCGCGTCTCCt                                                                             >  8:353606/1‑149 (MQ=255)
                                                                        tCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACt                                                                             >  4:39586/1‑149 (MQ=255)
                                                                         cAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACt                                                                             <  2:37685/148‑1 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  >  1:402054/1‑142 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  <  2:402054/142‑1 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  3:104991/1‑149 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  6:96908/1‑149 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  8:303549/1‑149 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  3:169558/1‑149 (MQ=255)
                                                                          aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           <  1:241096/149‑1 (MQ=255)
                                                                           gAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTTCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  2:119939/1‑148 (MQ=255)
                                                                                 aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGCACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTAc                                                                    >  6:74129/1‑149 (MQ=255)
                                                                                 aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgc                                                                                                               <  5:298936/106‑1 (MQ=255)
                                                                                 aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTa                                                                     >  2:375052/1‑148 (MQ=255)
                                                                                      aCAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                        <  2:98946/76‑2 (MQ=37)
                                                                                      aCAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                        >  1:98946/1‑75 (MQ=37)
                                                                                       cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGaa                                                              <  5:315399/149‑1 (MQ=255)
                                                                                        aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGa                                                                                             <  5:388789/117‑1 (MQ=255)
                                                                                        aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGa                                                                                             >  6:388789/1‑117 (MQ=255)
                                                                                        aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             <  7:303549/149‑1 (MQ=255)
                                                                                         aaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             >  1:227468/1‑148 (MQ=255)
                                                                                            cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         <  5:243203/149‑1 (MQ=255)
                                                                                              ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                       <  1:282446/149‑1 (MQ=255)
                                                                                               gAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTc                                                                            >  3:307556/1‑127 (MQ=255)
                                                                                               gAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTc                                                                            <  4:307556/127‑1 (MQ=255)
                                                                                                aaGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     <  1:108243/149‑1 (MQ=255)
                                                                                                  gTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGTGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     >  8:19090/1‑147 (MQ=255)
                                                                                                        cTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgc                                             >  1:368272/1‑149 (MQ=255)
                                                                                                             aCTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGGCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCAtt                                        <  7:384612/149‑1 (MQ=255)
                                                                                                               tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     <  4:331942/134‑1 (MQ=255)
                                                                                                               tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     >  3:331942/1‑134 (MQ=255)
                                                                                                               tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTAc                                      >  3:185536/1‑149 (MQ=255)
                                                                                                                       aTTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  5:197840/78‑1 (MQ=255)
                                                                                                                       aTTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     >  6:197840/1‑78 (MQ=255)
                                                                                                                        ttGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCTTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGc                             <  1:134718/149‑1 (MQ=255)
                                                                                                                        ttGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGc                             <  1:375052/149‑1 (MQ=255)
                                                                                                                         tGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCg                            >  5:157926/1‑149 (MQ=255)
                                                                                                                           tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGTGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGt                           <  7:19090/148‑1 (MQ=255)
                                                                                                                           tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                          >  2:339068/1‑149 (MQ=255)
                                                                                                                           tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGt                           <  2:227468/148‑1 (MQ=255)
                                                                                                                             aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGCCCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  6:369619/1‑149 (MQ=255)
                                                                                                                             aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  4:123602/1‑149 (MQ=255)
                                                                                                                             aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                         <  1:136288/148‑1 (MQ=255)
                                                                                                                             aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                         <  4:169558/148‑1 (MQ=255)
                                                                                                                              atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       >  6:122106/1‑149 (MQ=255)
                                                                                                                              atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       <  8:310398/149‑1 (MQ=255)
                                                                                                                                 atgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    <  1:228563/149‑1 (MQ=255)
                                                                                                                                   gtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgc                  >  7:406119/1‑149 (MQ=255)
                                                                                                                                     gcaaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGACCCATTGGCGCGTAACGTCGGATCCGAAGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAACTTTAAGCTTGCGCATTACCAGCGTGGCGTTGGTgccg                   >  1:117539/3‑146 (MQ=255)
                                                                                                                                      aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    <  4:353111/144‑1 (MQ=255)
                                                                                                                                      aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    >  3:353111/1‑144 (MQ=255)
                                                                                                                                      aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                >  7:46770/1‑148 (MQ=255)
                                                                                                                                      aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGCAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGAGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                >  7:116874/1‑148 (MQ=255)
                                                                                                                                       aaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa              <  1:339068/149‑1 (MQ=255)
                                                                                                                                        aaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaag             >  5:221399/1‑149 (MQ=255)
                                                                                                                                           gggAACCATGAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTTCCAGCGTGGCGTTGGTGCCgccgaagcc           <  1:212823/148‑1 (MQ=255)
                                                                                                                                             gAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccg          >  8:146287/1‑147 (MQ=255)
                                                                                                                                              aaCCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGccgaagccgaag       <  3:267235/149‑1 (MQ=255)
                                                                                                                                                ccATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCt     <  7:353606/149‑1 (MQ=255)
                                                                                                                                                ccATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCt     <  4:185536/149‑1 (MQ=255)
                                                                                                                                                  aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   <  5:96908/149‑1 (MQ=255)
                                                                                                                                                  aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   <  6:221399/149‑1 (MQ=255)
                                                                                                                                                   tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   >  2:328121/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGGAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440149‑2440444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: