Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,298 A→T 6.7% intergenic (‑178/+87) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,2980AT 6.7% 328.9 / 9.6 103intergenic (‑178/+87)yfcJ/fabBputative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (53/43);  new base T (3/4);  total (56/47)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 104 total aligned reads displayed.

GAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440151‑2440444
                                                                                                                                                   |                                                                                                                                                  
gagTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAg                                                                                                                                                   <  1:18975/149‑1 (MQ=255)
 agTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAgg                                                                                                                                                  <  4:366247/149‑1 (MQ=255)
        ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtt                                                                                                                                           <  6:84683/149‑1 (MQ=255)
         cGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttt                                                                                                                                          <  3:256468/149‑1 (MQ=255)
          ggctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTGTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         <  3:290852/147‑1 (MQ=255)
                tctctcTGGTTTCAGGGTTAAGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                   >  2:282446/1‑149 (MQ=255)
                    tctGGTTTGAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGc                                                                                                                                       <  8:2495/141‑1 (MQ=255)
                    tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGc                                                                                                                                       >  7:2495/1‑141 (MQ=255)
                    tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTa                                                                                                                                   <  1:312340/145‑1 (MQ=255)
                          ttCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTGAGTGCcggcgg                                                                                                                         >  4:120020/1‑149 (MQ=255)
                             aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttttcacat                                                                                                                                   <  4:359119/136‑7 (MQ=255)
                             aGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttttcacat                                                                                                                                   >  3:359119/1‑130 (MQ=255)
                                gTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAt                                                                                                                   <  8:342144/149‑1 (MQ=255)
                                gTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAt                                                                                                                   <  8:360388/149‑1 (MQ=255)
                                      gTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcgc                                                                                                             >  4:267235/1‑149 (MQ=255)
                                       tGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGcgcg                                                                                                            >  6:243203/1‑149 (MQ=255)
                                              gCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  4:24188/149‑1 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         <  5:191916/112‑1 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         >  6:191916/1‑112 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  8:105517/148‑1 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     >  7:105517/1‑148 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  2:134718/1‑149 (MQ=255)
                                               cAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  7:310398/1‑149 (MQ=255)
                                                aGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCg                                                                                                    >  1:37685/1‑148 (MQ=255)
                                                  gATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGAt                                                                                                 <  1:401223/149‑1 (MQ=255)
                                                    tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGCGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               >  1:389977/1‑149 (MQ=255)
                                                    tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               >  2:108243/1‑149 (MQ=255)
                                                    tttAAAGCGTTCTTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGc                                                                                               <  5:332010/149‑1 (MQ=255)
                                                        aCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACg                                                                                           >  2:241096/1‑149 (MQ=255)
                                                         cgcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGACAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGc                                                                                          >  2:212823/1‑149 (MQ=255)
                                                          gcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         >  7:164984/1‑149 (MQ=255)
                                                             tACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGgc                                                                                      >  5:234500/1‑149 (MQ=255)
                                                                gTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGcgcg                                                                                   >  2:136288/1‑149 (MQ=255)
                                                                 tCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  >  3:106091/1‑149 (MQ=255)
                                                                  cTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTc                                                                                 >  2:241604/1‑149 (MQ=255)
                                                                   ttttCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTc                                                                                 >  3:158995/1‑148 (MQ=255)
                                                                      tCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCTCGTAACGTCGGATGCGCCGCTGGCGCGTCTCCt                                                                             >  8:353606/1‑149 (MQ=255)
                                                                      tCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACt                                                                             >  4:39586/1‑149 (MQ=255)
                                                                       cAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACt                                                                             <  2:37685/148‑1 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  >  1:402054/1‑142 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  <  2:402054/142‑1 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           <  1:241096/149‑1 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  6:96908/1‑149 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  8:303549/1‑149 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  3:169558/1‑149 (MQ=255)
                                                                        aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  3:104991/1‑149 (MQ=255)
                                                                         gAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTTCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  2:119939/1‑148 (MQ=255)
                                                                               aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGCACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTAc                                                                    >  6:74129/1‑149 (MQ=255)
                                                                               aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgc                                                                                                               <  5:298936/106‑1 (MQ=255)
                                                                               aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTa                                                                     >  2:375052/1‑148 (MQ=255)
                                                                                  cGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGc                                                                 >  4:214195/1‑149 (MQ=255)
                                                                                    aCAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                        >  1:98946/1‑75 (MQ=37)
                                                                                    aCAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCttttt                                                                                                                                        <  2:98946/76‑2 (MQ=37)
                                                                                     cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGaa                                                              <  5:315399/149‑1 (MQ=255)
                                                                                      aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGa                                                                                             >  6:388789/1‑117 (MQ=255)
                                                                                      aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGa                                                                                             <  5:388789/117‑1 (MQ=255)
                                                                                      aaaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             <  7:303549/149‑1 (MQ=255)
                                                                                       aaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             >  1:227468/1‑148 (MQ=255)
                                                                                          cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         <  5:243203/149‑1 (MQ=255)
                                                                                            ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                       <  1:282446/149‑1 (MQ=255)
                                                                                             gAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTc                                                                            <  4:307556/127‑1 (MQ=255)
                                                                                             gAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTc                                                                            >  3:307556/1‑127 (MQ=255)
                                                                                              aaGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     <  1:108243/149‑1 (MQ=255)
                                                                                                gTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGTGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     >  8:19090/1‑147 (MQ=255)
                                                                                                      cTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgc                                             >  1:368272/1‑149 (MQ=255)
                                                                                                           aCTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGGCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCAtt                                        <  7:384612/149‑1 (MQ=255)
                                                                                                             tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     >  3:331942/1‑134 (MQ=255)
                                                                                                             tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCttt                                                     <  4:331942/134‑1 (MQ=255)
                                                                                                             tGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTAc                                      >  3:185536/1‑149 (MQ=255)
                                                                                                                     aTTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     <  5:197840/78‑1 (MQ=255)
                                                                                                                     aTTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTc                                                                                                     >  6:197840/1‑78 (MQ=255)
                                                                                                                      ttGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCTTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGc                             <  1:134718/149‑1 (MQ=255)
                                                                                                                      ttGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGc                             <  1:375052/149‑1 (MQ=255)
                                                                                                                       tGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCg                            >  5:157926/1‑149 (MQ=255)
                                                                                                                         tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGTGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGt                           <  7:19090/148‑1 (MQ=255)
                                                                                                                         tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                          >  2:339068/1‑149 (MQ=255)
                                                                                                                         tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGt                           <  2:227468/148‑1 (MQ=255)
                                                                                                                           aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGCCCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  6:369619/1‑149 (MQ=255)
                                                                                                                           aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  4:123602/1‑149 (MQ=255)
                                                                                                                           aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                         <  1:136288/148‑1 (MQ=255)
                                                                                                                           aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTg                         <  4:169558/148‑1 (MQ=255)
                                                                                                                            atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       <  8:310398/149‑1 (MQ=255)
                                                                                                                            atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       >  6:122106/1‑149 (MQ=255)
                                                                                                                               atgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    <  1:228563/149‑1 (MQ=255)
                                                                                                                                 gtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccgc                  >  7:406119/1‑149 (MQ=255)
                                                                                                                                   gcaaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGACCCATTGGCGCGTAACGTCGGATCCGAAGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAACTTTAAGCTTGCGCATTACCAGCGTGGCGTTGGTgccg                   >  1:117539/3‑146 (MQ=255)
                                                                                                                                    aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    <  4:353111/144‑1 (MQ=255)
                                                                                                                                    aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgcc                    >  3:353111/1‑144 (MQ=255)
                                                                                                                                    aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                >  7:46770/1‑148 (MQ=255)
                                                                                                                                    aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGCAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGAGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                >  7:116874/1‑148 (MQ=255)
                                                                                                                                     aaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa              <  1:339068/149‑1 (MQ=255)
                                                                                                                                      aaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaag             >  5:221399/1‑149 (MQ=255)
                                                                                                                                         gggAACCATGAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTTCCAGCGTGGCGTTGGTGCCgccgaagcc           <  1:212823/148‑1 (MQ=255)
                                                                                                                                           gAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagccg          >  8:146287/1‑147 (MQ=255)
                                                                                                                                            aaCCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGccgaagccgaag       <  3:267235/149‑1 (MQ=255)
                                                                                                                                              ccATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCt     <  7:353606/149‑1 (MQ=255)
                                                                                                                                                aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   <  5:96908/149‑1 (MQ=255)
                                                                                                                                                aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   <  6:221399/149‑1 (MQ=255)
                                                                                                                                                 tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   >  2:328121/1‑148 (MQ=255)
                                                                                                                                                   aGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   >  4:100145/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGTATCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440151‑2440444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: