Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 285,197 A→G 6.9% intergenic (+29/‑198) yagF → / → yagG CP4‑6 prophage; dehydratase family protein/CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913285,1970AG 6.9% 268.8 / 8.1 87intergenic (+29/‑198)yagF/yagGCP4‑6 prophage; dehydratase family protein/CP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base A (42/39);  new base G (2/4);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

ACCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACT  >  NC_000913/285052‑285344
                                                                                                                                                 |                                                                                                                                                   
acCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAata                                                                                                                                                  <  7:162581/149‑1 (MQ=255)
  ccGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATAtt                                                                                                                                                >  7:94609/1‑149 (MQ=255)
            gcggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGAc                                                                                                                                      <  1:369562/149‑1 (MQ=255)
              ggcACTGCAGTCGGTGAGCGGCGGCACCTGGACAGGCTGTATTTATGACACCGATAAACTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGa                                                                                                                                       <  1:25671/146‑1 (MQ=255)
              ggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAACTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGa                                                                                                                                       >  2:25671/1‑146 (MQ=255)
              ggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACgg                                                                                                                                    <  4:87509/149‑1 (MQ=255)
              ggcACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACgg                                                                                                                                    >  6:285443/1‑149 (MQ=255)
                     cAGTCGTTGAGCGGAGTCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGACGGttttatc                                                                                                                             <  2:66460/149‑4 (MQ=38)
                     cAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTACCGCAGACTGCtttttt                                                                                                                             >  8:285833/1‑149 (MQ=255)
                     cAGTCGGTGAGAGGCGGCACCTCGAAAGGCTGTATTTATGACACAGATAAAATTATAGAGGTAATTAAAGCAGGTAAAAAAGCGCTCGTAATTTAATTATTTTAAGAGATAAAACAGTCTGCGGAATATTTCCCGCAGACTGCAttgtt                                                                                                                             >  6:189562/1‑149 (MQ=255)
                           gTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGttttt                                                                                                                       <  2:344381/149‑1 (MQ=255)
                           gTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGttttt                                                                                                                       >  7:289064/1‑149 (MQ=255)
                            tGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGAc                                                                                                                                      <  2:131817/133‑1 (MQ=255)
                            tGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGGAAATATTCCGCAGAc                                                                                                                                      >  1:131817/1‑133 (MQ=255)
                            tGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTg                                                                                                                      <  7:377180/149‑1 (MQ=255)
                             gAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTg                                                                                                                      >  4:237376/1‑148 (MQ=255)
                                  gcggcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAAttt                                                                                                                <  8:84736/149‑1 (MQ=255)
                                  gcggcACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAAtt                                                                                                                 >  2:170670/1‑148 (MQ=255)
                                          tGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAAtt                                                                                                         >  5:121023/1‑148 (MQ=255)
                                          tGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAAtt                                                                                                         >  3:220076/1‑148 (MQ=255)
                                             aaaGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGTCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                     <  5:396513/149‑1 (MQ=255)
                                             aaaGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                     <  4:127858/149‑1 (MQ=255)
                                              aaGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                     <  5:266169/148‑1 (MQ=255)
                                                 gCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAAt                                                                                                 >  1:60728/1‑149 (MQ=255)
                                                     tATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTa                                                                                               <  5:285443/147‑1 (MQ=255)
                                                     tATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGt                                                                                             <  3:237376/149‑1 (MQ=255)
                                                     tATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGt                                                                                             <  5:255720/149‑1 (MQ=255)
                                                       tttATGACACCGATAAAATTTTCGAGGTTATTTACGCCGGTATAATAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATAATTCCCGCAGTCGGCTTTGTTGTTTTTGATATTTATTAATTTAAACAATTAGtt                                                                                            <  7:358082/148‑1 (MQ=255)
                                                         tATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTga                                                                                          <  3:6913/148‑1 (MQ=255)
                                                          aTGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGACTATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTgag                                                                                         <  7:285833/148‑1 (MQ=255)
                                                           tGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTgaga                                                                                        >  7:396204/1‑148 (MQ=255)
                                                           tGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTgaga                                                                                        <  8:396204/148‑1 (MQ=255)
                                                           tGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTgaga                                                                                        <  1:170670/148‑1 (MQ=255)
                                                              cacCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatata                                                                                    >  6:83788/1‑149 (MQ=255)
                                                               acCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatatat                                                                                   >  5:400301/1‑149 (MQ=255)
                                                               acCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTACCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGatatat                                                                                   >  4:63760/1‑149 (MQ=255)
                                                                ccGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATc                                                                                  >  6:27082/1‑149 (MQ=255)
                                                                ccGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATc                                                                                  >  8:394981/1‑149 (MQ=255)
                                                                 cGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCg                                                                                 >  3:333011/1‑149 (MQ=255)
                                                                 cGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCGTTGTTGTTTTTGATATTTATTATGTTAAACGAGTAGTTGCGatatatgg                                                                                 >  2:264984/1‑147 (MQ=255)
                                                                    tAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTg                                                                              <  8:216881/149‑1 (MQ=255)
                                                                    tAAAATTATAGCCGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGGCCGCGGAATAATTCCCGCAGTAGGCTTTGTTGGTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTg                                                                              <  8:300658/149‑1 (MQ=255)
                                                                       aaTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTa                                                                                               <  1:325912/129‑1 (MQ=255)
                                                                       aaTTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTa                                                                                               >  2:325912/1‑129 (MQ=255)
                                                                            tCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTcac                                                                       <  6:122364/148‑1 (MQ=255)
                                                                            tCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTcac                                                                       <  7:210614/148‑1 (MQ=255)
                                                                              gAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaaa                                                                    <  5:83788/149‑1 (MQ=255)
                                                                              gAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaa                                                                     >  7:386089/1‑148 (MQ=255)
                                                                               aGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaaaa                                                                   <  6:168391/149‑1 (MQ=255)
                                                                               aGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaaaa                                                                   <  4:220076/149‑1 (MQ=255)
                                                                               aGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaaaa                                                                   <  2:60728/149‑1 (MQ=255)
                                                                               aGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACaa                                                                     >  7:125418/1‑147 (MQ=255)
                                                                                    aTTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCaa                                                               >  7:164853/1‑148 (MQ=255)
                                                                                    aTTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCaa                                                               >  3:252724/1‑148 (MQ=255)
                                                                                        aCGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                     <  8:406688/106‑1 (MQ=255)
                                                                                        aCGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTaaa                                                                                                     >  7:406688/1‑106 (MQ=255)
                                                                                        aCGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATaa                                                          <  8:164853/149‑1 (MQ=255)
                                                                                             ggTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTTGCGTCACAAAAGCAAAATAACGTaa                                                     >  7:255454/1‑149 (MQ=255)
                                                                                             ggTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTaa                                                     >  5:321558/1‑149 (MQ=255)
                                                                                              gTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTaa                                                     <  2:162146/148‑1 (MQ=255)
                                                                                              gTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTaa                                                     >  1:162146/1‑148 (MQ=255)
                                                                                               tAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGTAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAAtt                                                   <  8:125418/149‑1 (MQ=255)
                                                                                                aaaaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTc                                                  >  8:309111/1‑149 (MQ=255)
                                                                                                  aaaaGCGCTCGGAATTTTATTATTTTAAGAGATAAAACCGTCTGCGGATTATTTCCCGCAGACGCCTTTGTTGTTTTTGAAATTAATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGAAAAATAACGTAATTCg                                                 >  8:182305/1‑148 (MQ=255)
                                                                                                  aaaaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAAGTAGTTGAGATATATCGTTGGCGTCACAAAAGCAACATAACGTAATTCg                                                 >  6:225787/1‑148 (MQ=255)
                                                                                                    aaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCAGAAAAGCAAAATAACTTAATTCGGa                                               >  1:58571/1‑148 (MQ=255)
                                                                                                    aaGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACTTAATTCGGa                                               >  1:108683/1‑148 (MQ=255)
                                                                                                           cGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGat                                       <  6:400301/149‑1 (MQ=255)
                                                                                                               aTTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGa                                   >  6:387545/1‑149 (MQ=255)
                                                                                                                 ttAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAATAGAAAAATAACGTAATTCGGAAATAAGATATGAcc                                 >  7:263422/1‑149 (MQ=255)
                                                                                                                 ttAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGAc                                  >  5:233603/1‑148 (MQ=255)
                                                                                                                   aaTTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCAt                               >  2:337970/1‑149 (MQ=255)
                                                                                                                          ttAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGATTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTTATATATATAGTTTGCGTCACAAAAGCAAAATAACTTAATTCGGAAATAATATATGACCATTGCTGGt                        >  2:281634/1‑149 (MQ=255)
                                                                                                                              gagaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGtt                                                                               <  6:90759/90‑1 (MQ=255)
                                                                                                                              gagaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGtt                                                                               >  5:90759/1‑90 (MQ=255)
                                                                                                                               agaTAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAAtt                   <  5:387545/149‑1 (MQ=255)
                                                                                                                                  aaaaaCCGTATGCGTAATATATCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAATATCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTTGTTAATTGaa                <  5:189562/148‑1 (MQ=255)
                                                                                                                                    aaaCCTTATTCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGATATATATCGTTGTCGTCACAAAAGCAAAATAACGTTATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAAt               <  2:139353/148‑1 (MQ=255)
                                                                                                                                    aaaCCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATa              <  8:386089/149‑1 (MQ=255)
                                                                                                                                    aaaCCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATa              <  4:333011/149‑1 (MQ=255)
                                                                                                                                     aaCCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAg             <  1:264984/149‑1 (MQ=255)
                                                                                                                                      aCCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGc            <  7:309111/149‑1 (MQ=255)
                                                                                                                                        cGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCt           >  4:216675/1‑148 (MQ=255)
                                                                                                                                         gTCTGCTGAATATTTCCCTCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCa         <  7:411901/149‑1 (MQ=255)
                                                                                                                                         gTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCa         >  6:400192/1‑149 (MQ=255)
                                                                                                                                               ggAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAATAGCAAAATAACGTAATTCGGAAATAAGATATGAGCATTGCTGGTTAATTGAATAGCTCATTacac   >  5:399104/1‑149 (MQ=255)
                                                                                                                                                gAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCATAATAAAGAAATTCCGAAATAAGATATGACCATTCCTGGTTAATTGAATAGATCATTACACt  >  6:23609/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATAAAACCGTCTGCGGAATATTTCCCGCAGACGGCTTTGTTGTTTTTGAAATTTATTAATTTAAACAATTAGTTGAGATATATCGTTGGCGTCACAAAAGCAAAATAACGTAATTCGGAAATAAGATATGACCATTGCTGGTTAATTGAATAGCTCATTACACT  >  NC_000913/285052‑285344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: