Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,684,983 A→T 5.5% S175S (TCT→TCA glyA ← serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,684,9830AT 5.5% 232.1 / 2.3 73S175S (TCT→TCAglyAserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (37/32);  new base T (2/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

TAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGAGTCAG  >  NC_000913/2684835‑2685129
                                                                                                                                                    |                                                                                                                                                  
tagtaACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCa                                                                                                                                                    <  5:149429/149‑1 (MQ=255)
tagtaACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCa                                                                                                                                                    <  4:181435/149‑1 (MQ=255)
  gtaACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCaga                                                                                                                                                  <  7:280894/149‑1 (MQ=255)
     acaacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAgaga                                                                                                                                                >  3:156464/1‑148 (MQ=255)
      caacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGaaa                                                                                                                                              >  2:338364/1‑149 (MQ=255)
           agatattGAGGAAACTGTTTCGGGTAGACTCCATCAGCAACCAGGACCGCAACTTGAGCCATATCAACGAACAGGTAAGCACAGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATCTGCAGAGAAaccac                                                                                                                                          <  2:84700/142‑1 (MQ=255)
            gAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAaccacc                                                                                                                                         >  7:105091/1‑148 (MQ=255)
             aGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGa                                                                                                                                       <  3:100469/149‑1 (MQ=255)
               cATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCaga                                                                                                                                                  <  4:150094/136‑1 (MQ=255)
               cATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCaga                                                                                                                                                  >  3:150094/1‑136 (MQ=255)
                  gAGGAACCGGGTTCGGGTAGACGCCAGCAGCAAACAGGCCCGCAACGTGAGCCATATCAACGAACAGGTAAGCTCCGATGATGTCAGCGATTTCAAGCATTTTCGACCAGTACACAAAGCCGGAATATGCAGAGAAaccac                                                                                                                                          >  1:84700/1‑141 (MQ=255)
                   aGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCa                                                                                                                                 >  3:103192/1‑149 (MQ=255)
                     gAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCAtt                                                                                                                               >  5:337383/1‑149 (MQ=255)
                     gAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCAtt                                                                                                                               >  3:75746/1‑149 (MQ=255)
                     gAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACAACCGATAATCAtt                                                                                                                               >  5:364550/1‑149 (MQ=255)
                      aaCCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCAttt                                                                                                                              <  8:155473/149‑1 (MQ=255)
                          gggTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCgg                                                                                                                          <  1:338364/149‑1 (MQ=255)
                            gatCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCCGAAAATGCAGAGCAACCACCGATAATCATTTTCGGCt                                                                                                                        >  2:133202/3‑149 (MQ=255)
                                     gACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCt                                                                                                                <  3:256177/148‑1 (MQ=255)
                                           agcagcAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCtt                                                                                                         <  4:156464/149‑1 (MQ=255)
                                            gcagcaACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTg                                                                                                        >  7:123931/1‑149 (MQ=255)
                                             cagcaACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGt                                                                                                       >  7:246570/1‑149 (MQ=255)
                                               gcaACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGttt                                                                                                     >  8:7332/1‑149 (MQ=255)
                                               gcaACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGGCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGttt                                                                                                     <  1:202302/149‑1 (MQ=255)
                                                    cAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                                                  >  7:329878/1‑147 (MQ=255)
                                                     aGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                                                  >  1:261675/1‑146 (MQ=255)
                                                         ccGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                                                  >  3:25819/1‑142 (MQ=255)
                                                           gCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGc                                                                                         <  6:337383/149‑1 (MQ=255)
                                                                    gcCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGat                                                                                <  8:70012/149‑1 (MQ=255)
                                                                        tatCAACGAACAGGTAAGCACCGATGCTGTCAGCGAGTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGa                                                                            >  7:85036/1‑149 (MQ=255)
                                                                            aaCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCTGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCgg                                                                        >  4:381737/1‑149 (MQ=255)
                                                                            aaCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCtt                                                                                                               >  6:283956/1‑110 (MQ=255)
                                                                            aaCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCtt                                                                                                               <  5:283956/110‑1 (MQ=255)
                                                                             aCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCgg                                                                        >  2:148056/1‑148 (MQ=255)
                                                                               gAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCTCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTTTTCTTTGGCTTGTTTTTCCAGATAGGCGTAGTCGATATGACCgg                                                                        >  4:279976/1‑146 (MQ=255)
                                                                                     gTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCTGTCCACCACTCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                                               <  7:355950/149‑1 (MQ=255)
                                                                                     gTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                                               >  7:412219/1‑149 (MQ=255)
                                                                                         gCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGAAGCATCGATAcc                                                           <  8:329878/149‑1 (MQ=255)
                                                                                           aCCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCCCCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCg                                                          >  8:255814/1‑148 (MQ=255)
                                                                                             ccaTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGt                                                         >  5:73817/3‑147 (MQ=255)
                                                                                             cGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGt                                                         >  7:280127/1‑147 (MQ=255)
                                                                                             cGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTaa                                                       <  7:7332/149‑1 (MQ=255)
                                                                                                   tGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGCAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGata                                                                               >  1:24824/1‑119 (MQ=255)
                                                                                                   tGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGata                                                                               <  2:24824/119‑1 (MQ=255)
                                                                                                   tGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTGCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCTTCGATACCGTAAGGacc                                                  >  1:260629/1‑146 (MQ=255)
                                                                                                     tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGCCCTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGAt                                               >  3:273324/1‑149 (MQ=255)
                                                                                                      cAGCGATTTCACTCATTTTCGCCCAGTCCACCACGCCTGTATATGCAGAGAAACCAGCGTTTATATTTTTCTGCTTGTGTTATTTGTCTTGTTTTTCCAGATATGCGTAGTCGATATGAGATGTAGCATCGATATCGTAAGGAACGAt                                               <  6:364550/148‑1 (MQ=255)
                                                                                                       aGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGATCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGAtgt                                             >  6:198972/1‑149 (MQ=255)
                                                                                                        gCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGAtgtt                                            <  8:123931/149‑1 (MQ=255)
                                                                                                         cGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGAtgttg                                           >  1:298965/1‑149 (MQ=255)
                                                                                                            tttCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCTGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTa                                         >  1:344195/1‑148 (MQ=255)
                                                                                                            attCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCTGAGAAACCTCCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTCTTTTTCCTGATGGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGCTGTTGTa                                         >  1:344192/2‑148 (MQ=255)
                                                                                                              tCACGCATTTTCGCCCAGTCCACCACTCCGGAATATGCAGAGAAACCACCTAAAATCATTTTCGGCTTGTGTTCTTTGGCTTGTGTTTCCAGATCGGCGGAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACa                                       <  4:56364/148‑1 (MQ=255)
                                                                                                               cACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAg                                      <  4:103192/148‑1 (MQ=255)
                                                                                                                    aTTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTAcc                                >  1:115776/1‑149 (MQ=255)
                                                                                                                     ttttCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCg                               <  8:412219/149‑1 (MQ=255)
                                                                                                                     ttttAGACCAGTCCACCACGCCTTAATATGCAGAGAAACCACAGATAATCATTTTCGGCTTGTGTTATTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCg                               <  3:279976/149‑1 (MQ=255)
                                                                                                                      tttCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGat                                                                                >  3:32229/1‑99 (MQ=255)
                                                                                                                      tttCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGat                                                                                <  4:32229/99‑1 (MQ=255)
                                                                                                                        tCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGga                             <  4:25819/148‑1 (MQ=255)
                                                                                                                          gCCCAGTCCACCACGCTGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGaa                          <  3:381737/149‑1 (MQ=255)
                                                                                                                          gCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGaa                          <  8:331740/149‑1 (MQ=255)
                                                                                                                             cAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGt                        >  8:393848/1‑148 (MQ=255)
                                                                                                                                tCCACCACGCCGGAATATGCTGAGAACCCCCCGATCATCATTTTCGGCTTGGGGTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCAGCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAAc                    <  2:344192/149‑1 (MQ=255)
                                                                                                                                tCCACCACGCCGGAATATGCTGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAAc                    <  2:344195/149‑1 (MQ=255)
                                                                                                                                  caccacGCCCGAATATGCAGAGAAACCACCGATAATCAGTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACAGGAGAATGTAACCg                  >  6:39070/1‑149 (MQ=255)
                                                                                                                                    ccacGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGTTGTTGTACAGTTTACCGGAGAAGTTAACCGga                <  6:73817/149‑1 (MQ=255)
                                                                                                                                        gCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGGTCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAAc            >  5:313108/1‑149 (MQ=255)
                                                                                                                                           ggATTATGCAGTGAAACCAACGATAATCATTTTCGGCTTGTGTTCTTTGGCGTGTTTTTCCTTTTCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTGCCGGAGAAGTTAACCGGAGAACCGt         <  1:133202/149‑1 (MQ=255)
                                                                                                                                           ggAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGt         <  1:148056/149‑1 (MQ=255)
                                                                                                                                           ggAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTGCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGt         <  4:75746/149‑1 (MQ=255)
                                                                                                                                             aaTATGCAGAGAAACCAGCGATAATCTTTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCGAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGa       <  2:260629/149‑1 (MQ=255)
                                                                                                                                             aaTATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGa       <  8:280127/149‑1 (MQ=255)
                                                                                                                                             aaTATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGa       <  2:115776/149‑1 (MQ=255)
                                                                                                                                               tatGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGAGt     >  2:130580/1‑149 (MQ=255)
                                                                                                                                                 tGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCTGAGAACCGTGa       >  7:21867/1‑145 (MQ=255)
                                                                                                                                                  gCAGAGAAACCACTGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGAGTCAg  >  4:282558/1‑149 (MQ=255)
                                                                                                                                                   cAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGAGTCAg  <  8:105091/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAGAAGTTAACCGGAGAACCGTGAGTCAG  >  NC_000913/2684835‑2685129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: