Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,981,039	T→C	5.0%	I382V (ATC→GTC)	araE ←	arabinose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,981,039	0	T	C	5.0%	259.4 / 3.2	81	I382V (ATC→GTC)	araE	arabinose transporter
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (2/2);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCACG  >  NC_000913/2980893‑2981187                                                                                                                                                   |                                                                                                                                                     gagCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATc                                                                                                                                                     >  7:290206/1‑148 (MQ=255) gagCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATc                                                                                                                                                     <  6:97985/148‑1 (MQ=255)  agCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCca                                                                                                                                                   <  2:216861/149‑1 (MQ=255)    ccAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcaca                                                                                                                                                 <  1:32694/149‑1 (MQ=255)        aaCGTACCTGCAGCGCCAATGCTATCAAGCAGTGTAAGGAAGGTCGCGCCGATAATCATATTCGACACCCATTTCGTTGTGGTCGAACATGTAATACCTAAATAGCTGCATTTCAGCTGCTGAATTTCAGAGCACATGATCCAcaccac                                                                                                                                             <  6:34233/149‑1 (MQ=255)             aCCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcg                                                                                                                                        >  5:355772/1‑149 (MQ=255)                       ccAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCAt                                                                                                                              >  1:236406/1‑149 (MQ=255)                        cAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATc                                                                                                                             >  1:195595/1‑149 (MQ=255)                          aTGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCg                                                                                                                            >  2:300820/1‑148 (MQ=255)                              tATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATaa                                                                                                                       >  4:169669/1‑149 (MQ=255)                              tATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATaa                                                                                                                       >  2:121779/1‑149 (MQ=255)                                tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATaa                                                                                                                       <  5:70667/147‑1 (MQ=255)                                tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAAcc                                                                                                                     >  1:385477/1‑149 (MQ=255)                                  aaGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg                                                                                                                   <  4:121410/149‑1 (MQ=255)                                  aaGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg                                                                                                                   >  3:24778/1‑149 (MQ=255)                                    gCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg                                                                                                                   >  6:75965/1‑147 (MQ=255)                                    gCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg                                                                                                                   <  5:75965/147‑1 (MQ=255)                                    gCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGc                                                                                                                  >  7:53989/1‑148 (MQ=255)                                     cAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCaa                                                                                                                <  6:355772/149‑1 (MQ=255)                                     cAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCaa                                                                                                                <  5:329863/149‑1 (MQ=255)                                       gtgtCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATa                                                                                                              <  6:301119/149‑1 (MQ=255)                                       gtgtCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATa                                                                                                              <  5:185570/149‑1 (MQ=255)                                          tCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATacac                                                                                                           <  6:161572/149‑1 (MQ=255)                                            aGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCTAACAGGTAATACCGAAATCTCGGCATTTCAGCGGCTGAATTTCATAGCACAGGATCCACACCACTGGCGCTGCGCTCATCGCATAACCCGCAATACAcat                                                                                                         >  8:167942/1‑149 (MQ=255)                                            aGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACAcat                                                                                                         >  7:383900/1‑149 (MQ=255)                                              taaGGTCGCGCCGATAATGATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACAGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACATGATCCACACCACTGGCGCGGCGCTCTTc                                                                                                                             <  1:50408/126‑1 (MQ=255)                                              gAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACAcatca                                                                                                       >  3:177436/1‑149 (MQ=255)                                              gAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACAcatca                                                                                                       <  8:290206/149‑1 (MQ=255)                                              gAAGGTCGCGCCGATAATAATATTCGACACCCAGTTAGTGGAGGACGATCAGGTAATAACGAAATCTCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGAACCACACCACTGGCGCGGCGCTCATc                                                                                                                             >  2:50408/1‑127 (MQ=255)                                               aaGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACAcatcat                                                                                                      <  8:383900/149‑1 (MQ=255)                                                  gTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGt                                                                                                   <  1:300820/149‑1 (MQ=255)                                                      cgcCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCACTCATCGCATAACCGGCAATACACATCATCGTCATg                                                                                               >  4:70664/1‑149 (MQ=255)                                                          gATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACCCCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCaa                                                                                           >  1:337527/1‑149 (MQ=255)                                                          gATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCaa                                                                                           >  3:18609/1‑149 (MQ=255)                                                            tAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACa                                                                                         <  8:53989/149‑1 (MQ=255)                                                                 atatTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACagaga                                                                                     <  2:385477/148‑1 (MQ=255)                                                                    ttCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCa                                                                                 <  4:24778/149‑1 (MQ=255)                                                                          acCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCa                                                                                 <  1:97682/143‑1 (MQ=255)                                                                          acCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCa                                                                                 >  2:97682/1‑143 (MQ=255)                                                                            ccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACaa                                                                           >  6:61777/1‑147 (MQ=255)                                                                            ccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACaa                                                                           <  5:61777/147‑1 (MQ=255)                                                                            ccAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGc                                                                         >  5:150279/1‑149 (MQ=255)                                                                            ccAGTTATTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACCGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCCATACACAGCATCGTCATGCCAACAGAGAGCCCGGACAAGc                                                                         >  2:307948/1‑149 (MQ=255)                                                                              aGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAACTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCa                                                                       >  7:136765/1‑149 (MQ=255)                                                                                 tCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACt                                                                     <  1:121779/148‑1 (MQ=255)                                                                                       tggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCa                                                                       >  8:368805/1‑140 (MQ=255)                                                                                       tggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCa                                                                       <  7:368805/140‑1 (MQ=255)                                                                                       tggtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc                                                              <  3:169669/149‑1 (MQ=255)                                                                                         gtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCg                                                             >  3:356932/1‑148 (MQ=255)                                                                                         gtCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCGCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCAACTGGAAGCCg                                                             >  6:345278/1‑148 (MQ=255)                                                                                          tCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTa                                                           <  4:18609/149‑1 (MQ=255)                                                                                            gAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTAcc                                                         >  7:327729/1‑149 (MQ=255)                                                                                            gAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTAcc                                                         >  1:318790/1‑149 (MQ=255)                                                                                                  gTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACCCCACTGGCGCGGCGCTCATCGCATACCCGGCAATCCACATCATCGTCATGCCAACAGAGCGCCAGGACAAGCCACTGGAAGCCGTACCGTTAt                                                    >  2:11875/1‑148 (MQ=255)                                                                                                  gTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTAt                                                    >  4:158279/1‑148 (MQ=255)                                                                                                       aCCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACt                                              >  2:39109/1‑149 (MQ=255)                                                                                                        aagAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCTTCGTCATGCCTACAGAGCGCCAGGACAAGCCACTGGAAGCCGTACCGTTGTCAGACTg                                             >  6:299412/3‑149 (MQ=255)                                                                                                         cGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGACGCCGTACCGTTATCAAACTg                                             >  3:315711/1‑148 (MQ=255)                                                                                                         cGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTg                                             >  3:304710/1‑148 (MQ=255)                                                                                                                gcgGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAgg                                      <  4:304710/148‑1 (MQ=255)                                                                                                                gcgGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAgg                                      >  8:323293/1‑148 (MQ=255)                                                                                                                 cgGCATTTCAGCGGCTGAATTTCAGAGCACAGGACCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc                                                              >  7:83395/1‑123 (MQ=255)                                                                                                                 cgGCATTTCAGCGGCTGAATTTCAGAGCACAGGACCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc                                                              <  8:133493/123‑1 (MQ=255)                                                                                                                 cgGCATTTCAGCGGCTGAATTTCAGAGCACAGGACCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc                                                              <  8:83395/123‑1 (MQ=255)                                                                                                                 cgGCATTTCAGCGGCTGAATTTCAGAGCACAGGACCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGcc                                                              >  7:133493/1‑123 (MQ=255)                                                                                                                  ggCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCaa                                   <  4:315711/149‑1 (MQ=255)                                                                                                                      tttCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGAACCGTTATCAAACTGCATCAGGCAATAGc                               <  2:195595/149‑1 (MQ=255)                                                                                                                          aGCGGCTGAATTTCAGATCACAGGATCCACACCACTGGCGCGGCACTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCa                            <  3:70664/148‑1 (MQ=255)                                                                                                                          aGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAg                           <  2:236406/149‑1 (MQ=255)                                                                                                                              gCTGAACTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCAcc                       <  8:136765/149‑1 (MQ=255)                                                                                                                               cTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCAccg                      <  1:198918/149‑2 (MQ=255)                                                                                                                               cTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCAccg                      >  2:198918/1‑148 (MQ=255)                                                                                                                                  aaTTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCaga                    <  6:150279/148‑1 (MQ=255)                                                                                                                                    tttCAGAGAACAGTATACACACCAATGGCGCGGCGCTCATCGAATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCAATGACAAGCCACTGTAAGCCGTACCGTTATCAAACTGCATCAGGAAATATCCAAGCACCAGAGTg                 >  6:170191/1‑149 (MQ=255)                                                                                                                                     ttCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGc                >  3:95792/1‑149 (MQ=255)                                                                                                                                       cAGAGCACAGGATCCCCACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTAGCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGcc               >  2:319569/1‑148 (MQ=255)                                                                                                                                            cacaGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGc         >  8:65712/1‑149 (MQ=255)                                                                                                                                               aGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGCGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCAAGCACCAGAGTGCCTAACGCCa       >  7:204392/1‑148 (MQ=255)                                                                                                                                               aGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCGTCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCAt      >  6:145018/1‑149 (MQ=255)                                                                                                                                                 gctCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCATCAGAGCGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCa    >  5:138153/3‑149 (MQ=255)                                                                                                                                                  aTCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCa    >  3:247836/1‑148 (MQ=255)                                                                                                                                                  aTCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCa    <  4:247836/148‑1 (MQ=255)                                                                                                                                                   tCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCa                            <  7:60631/123‑1 (MQ=255)                                                                                                                                                   tCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCa                            >  8:60631/1‑123 (MQ=255)                                                                                                                                                   tCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCACg  >  3:263631/1‑149 (MQ=255)                                                                                                                                                   tCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCACg  >  8:314281/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATACACATCATCGTCATGCCAACAGAGAGCCAGGACAAGCCACTGGAAGCCGTACCGTTATCAAACTGCATCAGGCAATAGCCCAGCACCAGAGTGCCTAACGCCATCACG  >  NC_000913/2980893‑2981187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑