Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,125,574 A→C 5.9% F317V (TTT→GTT)  glcE ← glycolate oxidase FAD binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,5740AC 5.9% 198.9 / 4.0 68F317V (TTT→GTT) glcEglycolate oxidase FAD binding subunit
Reads supporting (aligned to +/- strand):  ref base A (34/30);  new base C (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCG  >  NC_000913/3125431‑3125720
                                                                                                                                               |                                                                                                                                                  
cGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccac                                                                                                                                               <  7:94539/149‑1 (MQ=255)
        ttCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGGAAGGGTCGAGCTGCTGTTTAAGCTGCTTGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                                                                                       >  5:194884/1‑149 (MQ=255)
          cAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGCTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCgg                                                                                                                                      <  2:380846/148‑1 (MQ=255)
           aGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCa                                                                                                                                    <  2:61411/149‑1 (MQ=255)
             ttAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGAAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCAc                                                                                                                                   >  1:336146/1‑148 (MQ=255)
                   gggTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGc                                                                                                                             <  2:176033/148‑1 (MQ=255)
                   gggTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGc                                                                                                                             <  2:181849/148‑1 (MQ=255)
                    ggTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCgg                                                                                                                           <  2:198528/149‑1 (MQ=255)
                             aGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGa                                                                                                                  >  7:85810/1‑149 (MQ=255)
                                        cAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                                                                                       >  1:131711/1‑149 (MQ=255)
                                         aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccac                                                                                                                                               <  4:227697/108‑1 (MQ=255)
                                         aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccac                                                                                                                                               >  3:227697/1‑108 (MQ=255)
                                         aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                                                                                                <  4:178544/139‑1 (MQ=255)
                                         aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                                                                                                >  3:178544/1‑139 (MQ=255)
                                             ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                                                                                       >  7:173273/1‑112 (MQ=255)
                                             ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                                                                                       <  8:173273/112‑1 (MQ=255)
                                                  cgTACATGCGACCGTGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                                                                                             <  1:17617/149‑1 (MQ=255)
                                                       aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGcgatgcgatg                                                                                        >  8:205/1‑149 (MQ=255)
                                                        tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGcgatgcgatg                                                                                        >  3:147394/1‑148 (MQ=255)
                                                              cGGGGTTAAACACGCCTCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGTCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa                                                                                 <  7:91230/149‑1 (MQ=255)
                                                              cGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa                                                                                 <  2:336146/149‑1 (MQ=255)
                                                               ggggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt                                                                                <  8:346165/149‑1 (MQ=255)
                                                                 ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                                                                                            <  6:78340/135‑1 (MQ=255)
                                                                 ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                                                                                            >  5:78340/1‑135 (MQ=255)
                                                                     aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc                                                                                                                 >  7:263433/1‑110 (MQ=255)
                                                                     aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc                                                                                                                 <  8:263433/110‑1 (MQ=255)
                                                                       acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCCTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGAGATGCGATGGATTTGATTGTCCTCg                                                                        >  1:190879/1‑149 (MQ=255)
                                                                       acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCACATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg                                                                        >  7:293047/1‑149 (MQ=255)
                                                                       acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTACTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg                                                                        >  8:400698/1‑149 (MQ=255)
                                                                        cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGGCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCgg                                                                       <  5:80113/149‑1 (MQ=255)
                                                                                   ggTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTc                                                            <  4:147394/149‑1 (MQ=255)
                                                                                    gTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCACATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTca                                                           <  8:293047/149‑1 (MQ=255)
                                                                                     tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTcag                                                          >  4:56877/1‑149 (MQ=255)
                                                                                         gctgctGTTTAAGCTGCTGGTTATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAttt                                                                                   <  2:254589/120‑1 (MQ=255)
                                                                                         gctgctGTTTAAGATGCTGGAGATAGCGGAATAAAGGAGCCGATAGCCGGGAAAAGCCACCAACTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTTCGGGCGATGAGATGGAttt                                                                                   >  1:254589/1‑120 (MQ=255)
                                                                                                  tAAGCTACTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgc                                              <  7:400698/148‑1 (MQ=255)
                                                                                                     gctgctGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgccccg                                          >  2:184311/1‑149 (MQ=255)
                                                                                                      ctgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTTTCGATTTCAGCCAGCGTAAcgccccgc                                         >  8:136633/1‑149 (MQ=255)
                                                                                                        gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgccccgccc                                       >  1:19463/1‑149 (MQ=255)
                                                                                                         ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgccccg                                          <  6:53707/145‑1 (MQ=255)
                                                                                                         ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgccccg                                          >  5:53707/1‑145 (MQ=255)
                                                                                                          tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgccccgccc                                       >  8:188186/1‑147 (MQ=255)
                                                                                                             tGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTc                                  <  8:85810/149‑1 (MQ=255)
                                                                                                               aTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCACCGCCCCAGTCGa                                >  7:1742/1‑149 (MQ=255)
                                                                                                                tAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                               <  7:205/149‑1 (MQ=255)
                                                                                                                 aGCGGAATAAAGGAGCCGATAGCGGGGCAACGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                               >  6:135894/1‑148 (MQ=255)
                                                                                                                 aGCGGAATAAAGGAGCCGATAGCGGGGCAACGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                               >  6:141873/1‑148 (MQ=255)
                                                                                                                  gCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGAttt                                                             <  5:388705/117‑1 (MQ=255)
                                                                                                                  gCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGAttt                                                             >  6:388705/1‑117 (MQ=255)
                                                                                                                  gCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGAt                               >  3:329886/1‑147 (MQ=255)
                                                                                                                      aataatGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTg                         >  6:8657/1‑149 (MQ=255)
                                                                                                                      aaTAAAGGAGCCGATAGCGGGGCAACGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTg                         <  5:135894/149‑1 (MQ=255)
                                                                                                                      aaTAAAGGAGCCGATAGCGGGGCAACGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTg                         <  5:141873/149‑1 (MQ=255)
                                                                                                                       aTAAAGGAGCCGATAGCGGGGCGAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGc                        >  7:89443/1‑149 (MQ=255)
                                                                                                                       aTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGc                        >  4:38323/1‑149 (MQ=255)
                                                                                                                          aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCCGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCg                     >  5:158008/1‑149 (MQ=255)
                                                                                                                          aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCg                     >  2:61160/1‑149 (MQ=255)
                                                                                                                          aaGGAGCCGATAGCGGGGCAAAGCCACCATATCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCTGGCGATGCGATGGATTTGTTTGTGCTCGGCTGTCGTTTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTc                      <  6:194884/148‑1 (MQ=255)
                                                                                                                              aGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCgg                 <  1:184311/149‑1 (MQ=255)
                                                                                                                               gCCGATAGCGGGGCGAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCggg                <  8:89443/149‑1 (MQ=255)
                                                                                                                                 cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGt               >  8:36397/1‑148 (MQ=255)
                                                                                                                                 cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGt               >  5:205771/1‑148 (MQ=255)
                                                                                                                                   aTAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTaaa            >  6:156208/1‑149 (MQ=255)
                                                                                                                                         gggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatc      <  8:64357/149‑1 (MQ=255)
                                                                                                                                          ggCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatca     >  1:251486/1‑149 (MQ=255)
                                                                                                                                            cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCca        <  7:2011/144‑1 (MQ=255)
                                                                                                                                            cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCca        >  8:2011/1‑144 (MQ=255)
                                                                                                                                            cAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCatcatc   <  2:19463/149‑1 (MQ=255)
                                                                                                                                             aaaGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTTTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCg  <  7:136633/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCG  >  NC_000913/3125431‑3125720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: