| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 414,832 | G→T | 5.4% | H308N (CAT→AAT) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 414,832 | 0 | G | T | 5.4% | 386.8 / 7.4 | 110 | H308N (CAT→AAT) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (54/50); new base T (4/2); total (58/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
GGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCT > NC_000913/414685‑414980 | ggTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATgc > 3:11274/1‑149 (MQ=255)ggTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATgc > 8:193434/1‑149 (MQ=255)ggTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGGATTTCATTCTTCAATCTGCTGGCGAATATgc > 5:210477/1‑149 (MQ=255) gtTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGCATATTCGCCAg > 1:126359/1‑148 (MQ=255) cTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGCATATTCGCCAgcagat < 2:126359/149‑5 (MQ=255) tGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGcgccgc > 1:178361/1‑149 (MQ=255) tAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCGCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGcgccgc < 5:28852/147‑1 (MQ=255) aCCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtg > 3:175852/1‑149 (MQ=255) aCCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtg > 2:400017/1‑149 (MQ=255) aCCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt < 1:230183/148‑1 (MQ=255) aCCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt < 7:193434/148‑1 (MQ=255) ccAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgt > 7:184760/1‑149 (MQ=255) ccAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGT‑ACCGTGGATTTAATTCGTCAATAATCTGGCGAATTTGCGCCAGGGCCGCGCtggg > 1:90013/1‑147 (MQ=255) cAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgtg < 4:274802/149‑1 (MQ=255) cAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtgtg < 8:189978/149‑1 (MQ=255) gTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCGGGGTGGTGGCGAATGCTCGCGGGTTGCGGCATTGTGCTCTGTTAGCGAGTATTTTCTTCTTCATTCTGCTGGCGAATATGCGCCAGCGCGGCGCTGTGTTc > 3:197926/1‑149 (MQ=255) ttCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTg < 4:175852/148‑1 (MQ=255) aGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCTAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt < 7:165220/149‑1 (MQ=255) aGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt > 6:400096/1‑149 (MQ=255) aGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt > 5:117527/1‑149 (MQ=255) ggCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATg < 5:400096/149‑1 (MQ=255) ggCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATg < 1:400017/149‑1 (MQ=255) gCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGc < 8:184760/149‑1 (MQ=255) tgttgCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGt < 7:323426/147‑1 (MQ=255) gctgctgctGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCa < 8:215615/148‑1 (MQ=255) gctgctgctGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCa < 6:160358/148‑1 (MQ=255) tgctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTc < 7:168731/131‑1 (MQ=255) tgctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTc > 8:168731/1‑131 (MQ=255) ctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTAGTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAgtgt < 6:100861/148‑1 (MQ=255) gctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt < 4:152895/121‑1 (MQ=255) gctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCt > 3:152895/1‑121 (MQ=255) tgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt < 6:84490/131‑1 (MQ=255) tgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt > 5:84490/1‑131 (MQ=255) tgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAgtgt < 1:100434/144‑1 (MQ=255) tgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAgtgt > 2:100434/1‑144 (MQ=255) tgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGAc > 4:270229/1‑148 (MQ=255) aTTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACg > 5:282411/1‑145 (MQ=255) aTTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGa < 2:178361/149‑1 (MQ=255) ttCTGCTGACTGCTTCTCCGCGTGGTGGCTAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGGGGACGAAGAt < 1:319756/149‑1 (MQ=255) ttCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGa < 5:231626/142‑1 (MQ=255) ctgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTc < 6:117527/147‑1 (MQ=255) ctgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGt > 1:297873/1‑149 (MQ=255) tgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTg > 4:168241/1‑149 (MQ=255) gACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATgcgc < 2:338446/95‑1 (MQ=255) tGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAgt < 6:369581/127‑1 (MQ=255) tGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCgg > 5:869/1‑149 (MQ=255) cTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGtt > 5:1414/1‑149 (MQ=255) ttCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCa > 2:107588/1‑122 (MQ=255) ttCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCa < 1:107588/122‑1 (MQ=255) ttCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTtg > 5:401052/1‑149 (MQ=255) gCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGa > 7:61102/1‑115 (MQ=255) gCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGa < 8:61102/115‑1 (MQ=255) gCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGAc < 4:269088/116‑1 (MQ=255) gCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGAc > 3:269088/1‑116 (MQ=255) gAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAgcgc < 8:349613/148‑1 (MQ=255) gAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAgcgc > 7:349613/1‑148 (MQ=255) gAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgcc > 2:178139/1‑149 (MQ=255) cTCGCGCGAAGCGACATTGTGCTCTCTAAGCCAGAATATACTTCTTCAATCTCCTGGCCAATATGCGCCAGCGCCGCTCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAg < 4:197926/149‑1 (MQ=255) tCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAgcgcaacctc > 2:254584/1‑142 (MQ=255) tCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGt > 7:171683/1‑149 (MQ=255) gcgcgAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGtt > 3:70371/1‑148 (MQ=255) gcgcgAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGtt < 1:98279/148‑1 (MQ=255) gcgcCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc > 3:178238/1‑149 (MQ=255) aTTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttt < 4:70371/149‑1 (MQ=255) ttGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCtttt < 8:271527/149‑1 (MQ=255) tgCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttc < 1:390918/148‑1 (MQ=255) tgCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttc > 2:390918/1‑148 (MQ=255) ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt > 3:26879/1‑145 (MQ=255) ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt < 4:26879/145‑1 (MQ=255) tctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttct > 7:226601/1‑149 (MQ=255) ctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttctt > 4:186176/1‑149 (MQ=255) ctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttctt > 4:267452/1‑149 (MQ=255) tGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGAGTTATGAGATGCGTTCCCAGTGAGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGATGAGGTTGCGCTTTttcttcttc > 5:305601/1‑149 (MQ=255) tAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttcttc < 3:318357/147‑1 (MQ=255) tAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttcttc < 3:168241/147‑1 (MQ=255) tAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttctt < 3:186176/146‑1 (MQ=255) tAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCg < 6:1414/148‑1 (MQ=255) taGCGAGTATTTACTTCTTCAATCTGCTGGCCAATATGCGCCAGCGCCGCGCTGTTTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCgg < 2:90013/147‑1 (MQ=255) aGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGc < 6:401052/148‑1 (MQ=255) gAGTTTTTACTTCTGCTATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTACCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAAc < 6:305601/149‑1 (MQ=255) gAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGtt > 5:285171/1‑66 (MQ=255) gAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGtt < 6:285171/66‑1 (MQ=255) gAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAAc > 5:158813/1‑149 (MQ=255) aTTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCt > 1:259831/1‑149 (MQ=255) tttACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCt < 2:297873/148‑1 (MQ=255) aCTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGtt < 1:178139/148‑1 (MQ=255) cttcttCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGc < 6:255973/149‑1 (MQ=255) cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCaa < 3:267452/148‑1 (MQ=255) cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAg > 1:124674/1‑149 (MQ=255) aTCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCctg < 3:24336/149‑1 (MQ=255) tgGCGCATATTCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcg > 2:173086/1‑149 (MQ=255) tgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcg > 2:4885/1‑149 (MQ=255) gcgcATATTCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGtt < 8:203189/131‑1 (MQ=255) gAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCgg > 1:281621/1‑117 (MQ=255) gAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcggcgg > 1:251115/1‑149 (MQ=255) gAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcggcgg > 7:220543/1‑149 (MQ=255) gAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcggcgg > 8:392480/1‑149 (MQ=255) atGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCggctgg > 2:201240/1‑149 (MQ=255) tGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCggctggc < 7:81472/149‑1 (MQ=255) gcgcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCggctggct > 8:40149/1‑149 (MQ=255) | GGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCT > NC_000913/414685‑414980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |