Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 544,323 C→G 5.1% D324H (GAC→CAC)  allC ← allantoate amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913544,3230CG 5.1% 241.1 / 3.0 72D324H (GAC→CAC) allCallantoate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (30/37);  new base G (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATCATGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTACCCGGCAC  >  NC_000913/544176‑544464
                                                                                                                                                   |                                                                                                                                             
aaaTCATGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGtc                                                                                                                                               >  4:309429/1‑148 (MQ=255)
 aaTCATGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGtcca                                                                                                                                             >  3:117317/1‑149 (MQ=255)
 aaTCATGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGtcca                                                                                                                                             >  7:182883/1‑149 (MQ=255)
     aTGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGtccatcca                                                                                                                                         >  3:156309/1‑149 (MQ=255)
     aTGCAGGGTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGtccatcc                                                                                                                                          <  3:233792/148‑1 (MQ=255)
        cAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATa                                                                                                                                       >  4:318252/1‑148 (MQ=255)
           gTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATc                                                                                                                                   >  8:34916/1‑149 (MQ=255)
              ggTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTACATCCATAAATCGa                                                                                                                                 <  8:296588/148‑1 (MQ=255)
                tACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGata                                                                                                                               >  7:187416/1‑148 (MQ=255)
                  cgcgAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCa                                                                                                                            <  3:318252/149‑1 (MQ=255)
                     gAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATa                                                                                                                         >  2:175821/1‑149 (MQ=255)
                      aGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATAc                                                                                                                        <  4:156309/149‑1 (MQ=255)
                          gcgAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAAt                                                                                                                    <  7:278684/149‑1 (MQ=255)
                            gAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGt                                                                                                                  <  7:141267/149‑1 (MQ=255)
                            gAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGt                                                                                                                  >  7:166661/1‑149 (MQ=255)
                                aTTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCAt                                                                                                              <  4:270652/149‑1 (MQ=255)
                                     cgcgTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCAt                                                                                                         <  8:166661/149‑1 (MQ=255)
                                      gcgTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATc                                                                                                        <  8:182883/149‑1 (MQ=255)
                                          cgtGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTACATCGCa                                                                                                     >  6:75775/1‑148 (MQ=255)
                                            tGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTGCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAAt                                                                                                  >  3:365522/1‑149 (MQ=255)
                                            tGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTGCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAAt                                                                                                  >  3:359888/1‑149 (MQ=255)
                                                cGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGcc                                                                                              <  4:117317/149‑1 (MQ=255)
                                                 ggCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGccc                                                                                             <  5:278344/149‑1 (MQ=255)
                                                     ccacTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCAt                                                                                         <  1:161358/149‑1 (MQ=255)
                                                      cacTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATg                                                                                        >  1:302461/1‑149 (MQ=255)
                                                             cATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCAt                                                                                         >  2:236158/1‑141 (MQ=255)
                                                             cATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCAt                                                                                         <  1:236158/141‑1 (MQ=255)
                                                             cATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCATCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGtttt                                                                                 <  1:380665/149‑1 (MQ=255)
                                                                 aCCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTaa                                                                             <  3:309429/149‑1 (MQ=255)
                                                                   ccGGTAATTCAGTTTTTCTCTTTCAAACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGTTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACt                                                                           >  1:77110/1‑149 (MQ=255)
                                                                      gTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTACATCGCAAATCGCCCGCATGTCGTTTTCTAACTGtt                                                                        <  5:75775/149‑1 (MQ=255)
                                                                       tAATTCAGTTTTTCTCTTTCTCACAATTCTGTGAGGGTGGCGACGAGATCCTTTTTCATCGGCTCGGGTTCTTCGTCCTTCCATAAATCTATATCAATACCAATGTCCATTTCATCTCAAATCGGCCGGTTGTAGTTTTCTAACTGTTg                                                                       <  4:64188/149‑1 (MQ=255)
                                                                         aTTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTggg                                                                     >  5:376289/1‑149 (MQ=255)
                                                                         aTTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTgg                                                                      <  5:114739/148‑1 (MQ=255)
                                                                          ttCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGt                                                                    >  3:308050/1‑149 (MQ=255)
                                                                          ttCACTTTATCTCTTTCACACAAATCAGTCAGGGTGGCGACCGGCTCCTTATTCAACGGCACGGGTTCTTCGTCCATCCATAAATCGAAATCAAAACAAAAGTCCATTTCAACGCAAAACGACCGCATGTCGATTTCTAACTGTTGGGt                                                                    >  2:290418/1‑149 (MQ=255)
                                                                           tCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGt                                                                    >  3:188679/1‑148 (MQ=255)
                                                                           tCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGAACAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACAAATGTCCATTTCATCGCAAATCGCACGCATTTCGATTTATAACTGTTGGGt                                                                    >  4:249461/1‑148 (MQ=255)
                                                                              gTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGtt                                                                        >  4:300119/1‑141 (MQ=255)
                                                                              gTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGtt                                                                        <  3:300119/141‑1 (MQ=255)
                                                                                 tttCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGata                                                                                                                               >  3:89299/1‑83 (MQ=255)
                                                                                 tttCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGata                                                                                                                               <  4:89299/83‑1 (MQ=255)
                                                                                    ctctTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATcgcg                                                           <  5:24833/148‑1 (MQ=255)
                                                                                      ctTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAAGTGTTGGGTGAAATCGCgcag                                                        >  4:48269/1‑149 (MQ=255)
                                                                                       tttCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTGCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCgcgc                                                          <  4:359888/146‑1 (MQ=255)
                                                                                       tttCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTGCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGGCGTTTTCTAACTGTTGGGTGAAATCgcgc                                                          <  4:365522/146‑1 (MQ=255)
                                                                                           acacaATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATcgcg                                                           <  5:68022/141‑1 (MQ=255)
                                                                                           acacaATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATcgcg                                                           >  6:68022/1‑141 (MQ=255)
                                                                                            cacaATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGc                                                  <  6:376289/149‑1 (MQ=255)
                                                                                                      tCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCg                                           <  2:302461/146‑1 (MQ=255)
                                                                                                       cAGGGTGGCGACCATCTACTTATTCATCGGCACGGTTTCTTCGTCCATCCATATCTCGATATAAATACCAATGTCCATTTCATCGCAAAGCGCCCGCATGTCGGTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTAt                                       <  8:284939/149‑1 (MQ=255)
                                                                                                        aGGGTGGCGACCAGCTCCTTATTCATCGTCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTAt                                       <  3:249461/148‑1 (MQ=255)
                                                                                                          ggTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGAATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATgac                                    >  2:77830/1‑149 (MQ=255)
                                                                                                            tGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATgacgc                                  >  8:25959/1‑148 (MQ=255)
                                                                                                               cGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCACTGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGAcaa                               <  5:329119/149‑1 (MQ=255)
                                                                                                                   cAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGTAATCGCGCAGCACGGCAGCGTCGGTATGACGAcaatcaa                           >  6:52141/1‑149 (MQ=255)
                                                                                                                    aGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGAcaatcaat                          <  8:187416/149‑1 (MQ=255)
                                                                                                                      cTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACgg                                                   <  1:159221/122‑1 (MQ=255)
                                                                                                                      cTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACgg                                                   >  2:159221/1‑122 (MQ=255)
                                                                                                                      cTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATgg                        <  7:34916/149‑1 (MQ=255)
                                                                                                                         cTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACGAATGTCCATTTCATCGCAAATCTCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACTATCAATGGTg                      <  4:308050/148‑1 (MQ=255)
                                                                                                                         cTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTg                      >  4:14026/1‑148 (MQ=255)
                                                                                                                             ttCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGt                 >  1:56963/1‑149 (MQ=255)
                                                                                                                                aTCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCAc                                                     >  8:63203/1‑110 (MQ=255)
                                                                                                                                aTCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCAc                                                     <  7:63203/110‑1 (MQ=255)
                                                                                                                                aTCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGt              >  1:72132/1‑149 (MQ=255)
                                                                                                                                aTCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACAACAATCAATGGTGAACGTGGt              <  4:188679/149‑1 (MQ=255)
                                                                                                                                   ggCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCTCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGttt            <  2:56963/148‑1 (MQ=255)
                                                                                                                                      aCGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGt                                                                    <  6:80019/89‑1 (MQ=255)
                                                                                                                                      aCGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGt                                                                    >  5:80019/1‑89 (MQ=255)
                                                                                                                                       cGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTAccc       <  1:175821/149‑1 (MQ=255)
                                                                                                                                        gggTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATgg                        <  7:96235/131‑1 (MQ=255)
                                                                                                                                        gggTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATgg                        >  8:96235/1‑131 (MQ=255)
                                                                                                                                          gTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTACCCgg     >  8:45422/1‑148 (MQ=255)
                                                                                                                                             cttcGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTACCCGGcac  <  5:52141/148‑1 (MQ=255)
                                                                                                                                              ttcGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTgg               <  7:3719/134‑1 (MQ=255)
                                                                                                                                              ttcGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTgg               >  8:3719/1‑134 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AAATCATGCAGGTTGGTACGCGAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCTCTTTCACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCGATATCAATACCAATGTCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGCGCAGCACGGCAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTACCCGGCAC  >  NC_000913/544176‑544464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: