Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,054 C→T 42.8% Q50* (CAA→TAA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,0540CT42.8% 41.7 / 102.6 84Q50* (CAA→TAA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base T (16/20);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

GAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTG  >  NC_000913/755909‑756197
                                                                                                                                                 |                                                                                                                                               
gaAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAg                                                                                                                                              >  7:413390/1‑149 (MQ=255)
  aaTTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAggc                                                                                                                                            <  5:248068/149‑1 (MQ=255)
   aTTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAggcg                                                                                                                                           <  4:41053/149‑1 (MQ=255)
   aTTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAggcg                                                                                                                                           >  4:164951/1‑149 (MQ=255)
    ttGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAggcgg                                                                                                                                          >  8:45987/1‑149 (MQ=255)
       ccAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCAt                                                                                                                                       <  2:27442/149‑1 (MQ=255)
        cAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCAtt                                                                                                                                      <  6:247802/149‑1 (MQ=255)
          gTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTAc                                                                                                                                    >  1:168561/1‑149 (MQ=255)
          gTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGAGCAAGGCGGCATTAc                                                                                                                                    >  6:250074/1‑149 (MQ=255)
             agagAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCGAGGCGGCATTACCGt                                                                                                                                 >  1:39475/1‑149 (MQ=255)
             agagAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGt                                                                                                                                 >  4:207836/1‑149 (MQ=255)
              gagaATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCGAGGCGGCATTACCGtt                                                                                                                                <  2:39475/149‑1 (MQ=255)
                       aTGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGt                                                                                                                       <  6:186730/149‑1 (MQ=255)
                                tgATTGGTGCCGGTGGCGCAGGTATGCGCGCGTCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCa                                                                                                               <  2:108886/148‑1 (MQ=255)
                                 gATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCAt                                                                                                              >  5:341657/1‑148 (MQ=255)
                                   ttGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGaa                                                                                                           <  3:360176/149‑1 (MQ=255)
                                      gTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTACTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATa                                                                                                                   >  8:195276/1‑138 (MQ=255)
                                      gTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTACTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATa                                                                                                                   <  7:195276/138‑1 (MQ=255)
                                          cGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACt                                                                                                    >  6:56199/1‑149 (MQ=255)
                                           ggTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGAACCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTg                                                                                                   <  4:367033/149‑1 (MQ=255)
                                            gTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTgg                                                                                                  <  1:340158/149‑1 (MQ=255)
                                            gTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTgg                                                                                                  >  4:151934/1‑149 (MQ=255)
                                                cgcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAAt                                                                                              <  5:378146/149‑1 (MQ=255)
                                                 gcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATTAAGATAACTGGGaa                                                                                               >  7:132106/1‑147 (MQ=255)
                                                 gcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATTAAGATAACTGGGaa                                                                                               <  8:132106/147‑1 (MQ=255)
                                                   aGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGc                                                                                           <  8:322949/149‑1 (MQ=255)
                                                    ggTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCa                                                                                          <  1:313419/149‑1 (MQ=255)
                                                     gTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCat                                                                                         <  2:168561/149‑1 (MQ=255)
                                                     gTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCat                                                                                         <  6:78616/149‑1 (MQ=255)
                                                           gcgcgGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTAc                                                                                   >  5:24246/1‑149 (MQ=255)
                                                           gcgcgGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTAc                                                                                   <  7:45987/149‑1 (MQ=255)
                                                                   cTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGTAATGGCATATGTACGACACCGt                                                                           <  8:413390/149‑1 (MQ=255)
                                                                   cTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt                                                                           >  3:371187/1‑149 (MQ=255)
                                                                    tGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt                                                                           >  1:391045/1‑148 (MQ=255)
                                                                    tGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa                                                                                                      >  7:177321/1‑121 (MQ=255)
                                                                    tGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa                                                                                                      <  8:177321/121‑1 (MQ=255)
                                                                    tGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa                                                                                                      >  1:162062/1‑121 (MQ=255)
                                                                    tGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa                                                                                                      <  2:162062/121‑1 (MQ=255)
                                                                      cAAATTTCCCAGAGCGCCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCACATACAGTATCTCCGCAAGGCGCCATTACCCTTGCACTCGCTAATACCCATGAAGATAACTGCCAATGGCAAATGTACGACACCGTGa                                                                         >  6:395817/1‑148 (MQ=255)
                                                                       aaaTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGaaa                                                                       <  2:391045/149‑1 (MQ=255)
                                                                         aTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAgg                                                                     <  5:56199/149‑1 (MQ=255)
                                                                          tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGa                                                                                                         <  4:275684/112‑1 (MQ=255)
                                                                          tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGa                                                                                                         >  3:275684/1‑112 (MQ=255)
                                                                          tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCAAACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAggg                                                                    >  8:99599/1‑149 (MQ=255)
                                                                             cccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTc                                                                  <  4:371187/148‑1 (MQ=255)
                                                                              ccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTAGGACACCGTGaaa                                                                       >  3:275827/1‑142 (MQ=255)
                                                                              ccAGAGCGGCCACACCTGTGCGGTTGGCTCTAAAGTCTTCCCGTCCCGTTCCCATACCGTTTCTTCGTAATGCGGCATTACCGTTTCGCTGGGTAATACCCATGAATATAACTGGGAATGGCATATGTACGACACCGTGaaa                                                                       <  4:275827/142‑1 (MQ=255)
                                                                               cAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                               <  4:250411/149‑1 (MQ=255)
                                                                               cAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACAACGTGAAAGGGTCGGa                                                               >  1:375973/1‑149 (MQ=255)
                                                                               cAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                               >  1:33420/1‑149 (MQ=255)
                                                                                agagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCat                                                                                         >  4:299625/1‑122 (MQ=255)
                                                                                agagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCat                                                                                         <  3:299625/122‑1 (MQ=255)
                                                                                 gagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTgg                                                                                                  >  6:132225/1‑112 (MQ=255)
                                                                                 gagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTgg                                                                                                  <  5:132225/112‑1 (MQ=255)
                                                                                      gCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATTAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                        <  3:207836/149‑1 (MQ=255)
                                                                                      gCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGAGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                        <  5:250074/149‑1 (MQ=255)
                                                                                       ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCg                                                       >  5:357023/1‑149 (MQ=255)
                                                                                             cTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAc                                                  >  5:301547/1‑148 (MQ=255)
                                                                                               gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                               >  8:137033/1‑149 (MQ=255)
                                                                                                   gctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATa                                                                                                                   >  6:247141/1‑77 (MQ=255)
                                                                                                   gctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATa                                                                                                                   <  5:247141/77‑1 (MQ=255)
                                                                                                         ctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGCCACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtg                                      >  1:228074/1‑147 (MQ=255)
                                                                                                         ctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                      >  8:225045/1‑148 (MQ=255)
                                                                                                          tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt                                                                           <  5:305238/110‑1 (MQ=255)
                                                                                                          tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt                                                                           >  6:305238/1‑110 (MQ=255)
                                                                                                          tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                      <  7:182565/147‑1 (MQ=255)
                                                                                                            tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                   >  7:264942/1‑148 (MQ=255)
                                                                                                            tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                   >  8:248714/1‑148 (MQ=255)
                                                                                                              aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAat                                  <  6:24246/147‑1 (MQ=255)
                                                                                                              aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAat                                  <  5:104444/147‑1 (MQ=255)
                                                                                                              aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGTAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGaa                                   <  7:137033/146‑1 (MQ=255)
                                                                                                               aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                        >  6:134368/1‑124 (MQ=255)
                                                                                                               aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                        <  5:134368/124‑1 (MQ=255)
                                                                                                               aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatata                               >  2:194521/1‑149 (MQ=255)
                                                                                                                        gACCCGTTCACATACAGTTAATTCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCAATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAACAGGACGCGATTGAATATATGTGTaaaa                      >  4:63284/1‑149 (MQ=255)
                                                                                                                           ccGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTg                                     >  6:312590/1‑131 (MQ=255)
                                                                                                                           ccGTTCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTg                                     <  5:312590/131‑1 (MQ=255)
                                                                                                                              ttCCCATACCGTTTCTGCGTAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                <  8:264942/149‑1 (MQ=255)
                                                                                                                                cccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc               <  3:164951/148‑1 (MQ=255)
                                                                                                                                 ccATACCGTTTCTGCGCAAGGCGGCATTACCGTAGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTAGGACACCGTGAAAGGGTCGGACAATATCGGTGACCAGGACGCGATAGAATATATGTGTAAAACCGGGCCg              >  7:366051/1‑148 (MQ=255)
                                                                                                                                    tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg          <  1:17386/149‑1 (MQ=255)
                                                                                                                                        gattCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt      >  1:94478/3‑149 (MQ=255)
                                                                                                                                         tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATTAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt     <  6:341657/149‑1 (MQ=255)
                                                                                                                                            cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg  <  2:33420/149‑1 (MQ=255)
                                                                                                                                               cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg  <  7:248714/146‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTG  >  NC_000913/755909‑756197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: