Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,046,842 C→A 5.1% T368T (ACG→ACT gfcD ← putative O‑antigen capsule production periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,046,8420CA 5.1% 341.7 / 3.0 101T368T (ACG→ACTgfcDputative O‑antigen capsule production periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (47/48);  new base A (3/3);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GCCCCGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTCTGGCGCGTCA  >  NC_000913/1046695‑1046989
                                                                                                                                                   |                                                                                                                                                   
gCCCCGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCGGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGc                                                                                                                                                     <  7:187929/148‑1 (MQ=255)
  cccGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGAGt                                                                                                                                                   >  1:278948/1‑148 (MQ=255)
   ccGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCa                                                                                                                                                 <  2:54315/149‑1 (MQ=255)
    cGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCAc                                                                                                                                                <  2:350057/149‑1 (MQ=255)
    cGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCAc                                                                                                                                                >  2:43733/1‑149 (MQ=255)
     gACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACg                                                                                                                                               <  6:160125/149‑1 (MQ=255)
         aaaGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTaa                                                                                                                                           >  1:213142/1‑149 (MQ=255)
         aaaGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTaa                                                                                                                                           <  5:34893/149‑1 (MQ=255)
          aaGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAt                                                                                                                                          <  2:213142/149‑1 (MQ=255)
          aaGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCACCAGTTAGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCCCCATATGCTCGCGCTATTGCGTCACGCTAAt                                                                                                                                          >  5:231942/1‑149 (MQ=255)
              cAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                                                                                                      >  1:131255/1‑149 (MQ=255)
              cAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                                                                                                      >  7:95286/1‑149 (MQ=255)
               aGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttct                                                                                                                                     <  3:9600/149‑1 (MQ=255)
                gAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGc                                                                                                                                              <  2:57917/139‑1 (MQ=255)
                gAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGc                                                                                                                                              >  1:57917/1‑139 (MQ=255)
                gAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTtctc                                                                                                                                    <  3:252184/149‑1 (MQ=255)
                 aaaGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTtctc                                                                                                                                    >  5:60459/1‑148 (MQ=255)
                     aTCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGc                                                                                                                               >  4:178213/1‑149 (MQ=255)
                          cAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGcc                                                                                                                              <  2:361521/145‑1 (MQ=255)
                          cAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTg                                                                                                                           <  1:171256/148‑1 (MQ=255)
                           aGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGTCGCTGTACCTGCCAGCTGTTAGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCg                                                                                                                         >  3:241387/1‑149 (MQ=255)
                           aGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCg                                                                                                                                                    <  3:347309/122‑1 (MQ=255)
                           aGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCg                                                                                                                                                    >  4:347309/1‑122 (MQ=255)
                           aGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCg                                                                                                                         >  4:307940/1‑149 (MQ=255)
                           aGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCg                                                                                                                         >  2:147074/1‑149 (MQ=255)
                            gCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCgg                                                                                                                        >  2:186833/1‑149 (MQ=255)
                            gCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGATACGTCGGTTTCGGTCGTAACCATAGCCATATTCTCGCGCTTTTGAGTAACGCTACTCTTCTCAACGCCTTGAgg                                                                                                                        >  8:283596/1‑149 (MQ=255)
                                cAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAgg                                                                                                                    >  2:31963/1‑149 (MQ=255)
                                 aaCACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAgg                                                                                                                    <  2:343190/148‑1 (MQ=255)
                                       ttgttgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCa                                                                                                             <  8:11457/149‑1 (MQ=255)
                                          ttgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCa                                                                                                          <  1:43733/149‑1 (MQ=255)
                                            gttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAg                                                                                                         >  8:192456/1‑148 (MQ=255)
                                               gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCTGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                                                                                                      <  2:107826/116‑1 (MQ=255)
                                               gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                                                                                                      >  1:107826/1‑116 (MQ=255)
                                               gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCACCGCCTTGCGGCAGGTTATTCACCAGAATc                                                                                                     >  6:11888/1‑149 (MQ=255)
                                               gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGc                                                                                                                          >  3:131820/1‑128 (MQ=255)
                                               gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGc                                                                                                                          <  4:131820/128‑1 (MQ=255)
                                                     gCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGtt                                                                                                <  3:307940/148‑1 (MQ=255)
                                                     gCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGtt                                                                                                <  1:147074/148‑1 (MQ=255)
                                                     gCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGtt                                                                                                <  1:31963/148‑1 (MQ=255)
                                                     gCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTg                                                                                               >  1:68187/1‑149 (MQ=255)
                                                            ttGACCAGGCGCTGTACCTGCCAGCTGTGTGCGCAGGCTGGCGACGGAGGTTTCGTTAGGCACCATCGCCAACTGCTCGCGCTTATGCGTCACGCTAATCTTCTCAACGCCTTGCTGCAGTTTATTCACCAGAATCCGGTTGGCACGAt                                                                                        <  7:283596/149‑1 (MQ=255)
                                                               aCCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGa                                                                                     >  2:401447/1‑149 (MQ=255)
                                                               aCCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGa                                                                                     >  1:148001/1‑149 (MQ=255)
                                                               aCCAGGCGCTGTACCGGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGACACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGa                                                                                     <  1:328620/149‑1 (MQ=255)
                                                                ccAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCAcc                                                                                                           >  5:112850/1‑126 (MQ=255)
                                                                ccAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGAGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCAcc                                                                                                           <  6:112850/126‑1 (MQ=255)
                                                                 cAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAcc                                                                                   >  1:32442/1‑149 (MQ=255)
                                                                 cAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAcc                                                                                   >  7:118506/1‑149 (MQ=255)
                                                                  aGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAtt                                                                                                               >  1:60437/1‑120 (MQ=255)
                                                                  aGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAtt                                                                                                               <  2:60437/120‑1 (MQ=255)
                                                                      gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACCCCATGCGGCAGGTTATTCACCAGAATCCGCTTGGCCCGATCGACCGCTTc                                                                              >  7:196468/1‑149 (MQ=255)
                                                                       cTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCa                                                                             <  8:154164/149‑1 (MQ=255)
                                                                        tGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCAc                                                                            >  7:208512/1‑149 (MQ=255)
                                                                        tGTACCTGCCAGCTGTTTGCGCAGGCGGGCTACGTCGGTTTCGGTAGGCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGGTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCAc                                                                            <  3:59468/149‑1 (MQ=255)
                                                                          tACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGAGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCAc                                                                            <  2:278948/147‑1 (MQ=255)
                                                                          tACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGAGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCAc                                                                            <  6:78294/147‑1 (MQ=255)
                                                                           aCCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCAc                                                                            >  2:34898/1‑146 (MQ=255)
                                                                            ccTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACgaga                                                                        <  8:208512/149‑1 (MQ=255)
                                                                             cTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGagag                                                                       >  5:25158/1‑149 (MQ=255)
                                                                              tGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCa                                                                                                                                   >  1:383684/1‑88 (MQ=255)
                                                                              tGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCa                                                                                                                                   <  2:383684/88‑1 (MQ=255)
                                                                                 cAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCAc                                                                   <  2:68187/149‑1 (MQ=255)
                                                                                  aGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACg                                                                  >  1:367398/1‑149 (MQ=255)
                                                                                       tttGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTAtt                                                             <  3:178213/149‑1 (MQ=255)
                                                                                       tttGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTAt                                                              <  8:118506/148‑1 (MQ=255)
                                                                                        ttGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTAttt                                                            >  3:65019/1‑149 (MQ=255)
                                                                                         tGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTAttt                                                            <  1:186833/148‑1 (MQ=255)
                                                                                                gctggctACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCt                                                    >  4:2856/1‑149 (MQ=255)
                                                                                                  tggctACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGa                                                  >  1:27084/1‑149 (MQ=255)
                                                                                                   ggctACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGAc                                                 <  3:2856/149‑1 (MQ=255)
                                                                                                    gctACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGa                                                  <  1:401447/147‑1 (MQ=255)
                                                                                                      tACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAc                                                                                    <  1:287738/111‑1 (MQ=255)
                                                                                                      tACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAc                                                                                    >  2:287738/1‑111 (MQ=255)
                                                                                                        cGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCTGCAGGTTATTCACCAGAATCCGGTTTGCACGATCGACCGCTTCAAGAGAGTCACTGTATTTGTATTGCTGACCATAc                                            <  4:241387/149‑1 (MQ=255)
                                                                                                        cGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGa                                             <  1:397589/148‑1 (MQ=255)
                                                                                                         gTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGt                                                                >  3:52725/1‑128 (MQ=255)
                                                                                                         gTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGt                                                                <  4:52725/128‑1 (MQ=255)
                                                                                                         gTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACa                                           <  2:349170/149‑1 (MQ=255)
                                                                                                          tCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACa                                           >  4:182881/1‑148 (MQ=255)
                                                                                                             gTTTCTGTAGTCAGCTTCGCCATATGCTCGCGGTTTTGCGTCACGCTAATCTTCTCAACGCCGTGCGGCAGGTTATTCAACAGAATCCTGTTGGAACGATCTACCGCTTCACGAGAGTCTCGGTATTTGTATTGCTGACCTGACAtata                                       <  8:196468/149‑1 (MQ=255)
                                                                                                             gTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGGTATTCACCAGAATCCGGTTGGCGCGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACGTGGCAtata                                       >  4:232491/1‑149 (MQ=255)
                                                                                                              tttCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGCGAGTCACGGTATTTGTATTGCTGACCAGACATATAc                                      <  6:60459/149‑1 (MQ=255)
                                                                                                                 cGGTCGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAg                                    <  1:191373/148‑1 (MQ=255)
                                                                                                                   gTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAgtgt                                 <  4:65019/149‑1 (MQ=255)
                                                                                                                       tCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTa                             >  4:166016/1‑149 (MQ=255)
                                                                                                                         aCCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATc                           >  8:52429/1‑149 (MQ=255)
                                                                                                                         aCCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATc                           >  5:354680/1‑149 (MQ=255)
                                                                                                                         aCCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATc                           <  7:192456/149‑1 (MQ=255)
                                                                                                                                ccATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTg                                                      <  4:291579/115‑1 (MQ=255)
                                                                                                                                ccATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGc                    >  5:302126/1‑149 (MQ=255)
                                                                                                                                 cATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCACCGCCTTGCGGCAGGTCATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAg                     <  2:291589/147‑1 (MQ=255)
                                                                                                                                 cATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCt                   >  1:285140/1‑149 (MQ=255)
                                                                                                                                       cTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAAttt             <  7:52429/149‑1 (MQ=255)
                                                                                                                                        tCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGCATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTc            >  1:54934/1‑149 (MQ=255)
                                                                                                                                        tCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCa                                               >  7:391489/1‑114 (MQ=255)
                                                                                                                                        tCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCa                                               <  8:391489/114‑1 (MQ=255)
                                                                                                                                                  gCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTCTGGCGCGTc   >  1:19142/1‑148 (MQ=255)
                                                                                                                                                   cGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCCCGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTCTGGCGCGTCa  >  6:105227/1‑148 (MQ=255)
                                                                                                                                                   cGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTCTGGCGCGTCa  >  5:82699/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCCCGACCAAAGGCAGAAAGATCTTCAGCTTCAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGAGAGTCACGGTATTTGTATTGCTGACCAGACATATACAGTGTCTTATCGCGTAGCTGAATTTCTGGCGCGTCA  >  NC_000913/1046695‑1046989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: