Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,375,962 C→G 5.1% Q334E (CAG→GAG)  ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,375,9620CG 5.1% 297.5 / 3.2 90Q334E (CAG→GAG) ycjQputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (45/39);  new base G (2/3);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGG  >  NC_000913/1375817‑1376110
                                                                                                                                                 |                                                                                                                                                    
cAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGTAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAg                                                                                                                                                    <  8:279257/148‑1 (MQ=255)
cAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGt                                                                                                                                                   <  6:217070/149‑1 (MQ=255)
 aGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGGAGTa                                                                                                                                                  >  3:71585/1‑149 (MQ=255)
  gCGAACCGCACCCGGATAATCCGCGCTGGCGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCAGATGACCGGTTATCTCAATAGAGAAGATTTAATCGACCCGGTGGTCACCGTTGCCAATCGCCCGGAAAGCTATATGCAGt                                                                                                                                                   >  2:327170/1‑147 (MQ=255)
  gCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCa                                                                                                                                                     >  1:202517/1‑145 (MQ=255)
  gCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCa                                                                                                                                                     <  2:202517/145‑1 (MQ=255)
             ccGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCAAATATCCCGGAAAGCTATATGAAGGATGTCGAACAGCa                                                                                                                                      >  6:306514/1‑149 (MQ=255)
                            tGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCAt                                                                                                                       >  2:293526/1‑149 (MQ=255)
                              gAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATc                                                                                                                      >  7:70470/1‑148 (MQ=255)
                              gAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCa                                                                                                                     <  2:187913/149‑1 (MQ=255)
                               aGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCa                                                                                                                     >  6:206389/1‑148 (MQ=255)
                                gCCGCAAGCGTATTGAAGAAACCTGTTTGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCACGGAAATCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCaa                                                                                                                    >  6:322274/1‑148 (MQ=255)
                                   gCAAGGGTATTGAAGAAACCTGTTGGTAACTGCTGATGAACGTTTATCTCAATATCGAAGATTTAATCGACCCAGTGGTGACCTTTGACAATAGCCCGGAAAGCTATATACAGTATGTCGACCAGCATCCGGAACAGAGCAACaaaagt                                                                                                                >  5:243922/1‑147 (MQ=255)
                                   gCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATg                                                                                                                >  3:159741/1‑149 (MQ=255)
                                      aGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGGAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGc                                                                                                             <  4:71585/149‑1 (MQ=255)
                                       gCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCg                                                                                                            >  3:176058/1‑149 (MQ=255)
                                         gTATTCAAGAAACCTGTTGGGAACTGCTGTTGAACGTTTATCTCAATTTCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCTATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCTTAACAGAGCATCAAAATGGGCGtt                                                                                                          <  7:199896/149‑1 (MQ=255)
                                           aTTGAAGAAAACTGATGGGAACTGCTGAAGAACGGTAATCTCAATTGAGAAGATTTAATCGACCCGGTGGTGACCTTTCCCAATATCACGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGACCATCAAAATGGCCGTAAc                                                                                                        >  3:61253/1‑149 (MQ=255)
                                              gaagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGtt                                                                                                     >  2:389339/1‑149 (MQ=255)
                                               aagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGACGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGttt                                                                                                    <  2:111579/149‑1 (MQ=255)
                                               aagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGAGCAGCATCCGGAACaga                                                                                                                           >  3:26602/1‑126 (MQ=255)
                                               aagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACaga                                                                                                                           <  4:26602/126‑1 (MQ=255)
                                               aagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGttt                                                                                                    >  7:37953/1‑149 (MQ=255)
                                               aagaaACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGtt                                                                                                     <  1:293526/148‑1 (MQ=255)
                                                  aatcggtttGTGAATTTCTGATTAATGGTTATCTCAATGTGGATGATTTAATCGACCAGGTGGTGACCTTTGCCAATAGCACGTAAAGCTATATGGAGTATGTCGACCAGCATCCGGAACAGTGCATCAAAATGGTCGTTACGTTTTaa                                                                                                 <  6:243922/142‑1 (MQ=255)
                                                  aaaCCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTaa                                                                                                 >  7:15017/1‑149 (MQ=255)
                                                    aCCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGCAACAGAGCATCAAAATGGGCGTTACGTTTTAAt                                                                                                >  5:73871/1‑148 (MQ=255)
                                                         ttGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCaaa                                                                                           <  6:73871/148‑1 (MQ=255)
                                                         ttGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCaaa                                                                                           <  1:9514/148‑1 (MQ=255)
                                                         ttGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAg                                                                                          <  4:51045/149‑1 (MQ=255)
                                                         ttGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAg                                                                                          >  3:195567/1‑149 (MQ=255)
                                                           gggAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAg                                                                                          >  8:294462/1‑147 (MQ=255)
                                                           gggAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAg                                                                                          <  7:294462/147‑1 (MQ=255)
                                                                 tgctgATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGaa                                                                                   >  3:114571/1‑148 (MQ=255)
                                                                    tgatgaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGaaaaa                                                                                >  3:371775/1‑148 (MQ=255)
                                                                     gatgaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGtt                                                                                                          <  7:85921/121‑1 (MQ=255)
                                                                     gatgaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGtt                                                                                                          >  8:85921/1‑121 (MQ=255)
                                                                      atgaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGaaaaaa                                                                               <  5:206389/147‑1 (MQ=255)
                                                                        gaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATg                                                                            >  7:325667/1‑148 (MQ=255)
                                                                        gaACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCAGGAAAGCTATATGCAGTATGTCCACCAGCATCCGGAACCGAGCATCAAAATGGGCGTAACGTTTTAATTCAAAGGAATGAAAAAAAtt                                                                            >  2:213503/1‑147 (MQ=255)
                                                                          aCGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGCCCTTTGCCAATAGCCCGGCAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGaa                                                                          >  8:298271/1‑148 (MQ=255)
                                                                          aCGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGaa                                                                          >  2:196424/1‑148 (MQ=255)
                                                                           cGGTTATCTCAATTGCGAAGATTTAATCGCCCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGAATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGaaaa                                                                        >  5:265328/1‑149 (MQ=255)
                                                                            ggTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAAt                                                                       >  8:239769/1‑149 (MQ=255)
                                                                                aTCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGTCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCgg                                                                    <  5:306514/148‑1 (MQ=255)
                                                                                   tcAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACAAGCATCCGGAAAATAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGcaac                                                                >  6:357377/1‑147 (MQ=255)
                                                                                           gAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGCCCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTGTAATTCAAAGGAATGAAAAAAATGAAAATCGGCAGACAGAAt                                                          >  1:285258/1‑147 (MQ=255)
                                                                                           gAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAAt                                                          >  6:151222/1‑147 (MQ=255)
                                                                                           gAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCa                                                        <  1:389339/149‑1 (MQ=255)
                                                                                            aaGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACa                                                                                                                             <  4:119254/79‑1 (MQ=255)
                                                                                            aaGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACa                                                                                                                             >  3:119254/1‑79 (MQ=255)
                                                                                            aaGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAg                                                       >  2:222997/1‑149 (MQ=255)
                                                                                            aaGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATAAAAAAAATGAAAATCGGCACACAGatgcag                                                       >  2:40711/1‑144 (MQ=255)
                                                                                             aGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAgg                                                      <  8:254556/149‑1 (MQ=255)
                                                                                              gATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGc                                                     <  3:293524/149‑1 (MQ=255)
                                                                                              gATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGc                                                     <  6:120875/149‑1 (MQ=255)
                                                                                               aTTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGGAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATc                                                         >  2:176864/1‑144 (MQ=255)
                                                                                               aTTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGGAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATc                                                         <  1:176864/144‑1 (MQ=255)
                                                                                               aTTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATTGCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAAt                                                                                                <  7:213672/105‑1 (MQ=255)
                                                                                               aTTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGATACGTTTTAAt                                                                                                >  8:213672/1‑105 (MQ=255)
                                                                                                tttAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGt                                                   >  2:171391/1‑149 (MQ=255)
                                                                                                    aTCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCt                                                <  1:222997/148‑1 (MQ=255)
                                                                                                     tCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAATCTATATGCAGTATGTCGAACAGCATCCGGAACAGAGCATCAAAATTGGCGTTACGTTTTACTTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTGCttt                                              <  5:322274/149‑1 (MQ=255)
                                                                                                     tCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCttt                                              >  2:20095/1‑149 (MQ=255)
                                                                                                       gACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTcc                                            <  5:151222/149‑1 (MQ=255)
                                                                                                       gACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTc                                             <  4:114571/148‑1 (MQ=255)
                                                                                                       gACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTc                                             <  4:195567/148‑1 (MQ=255)
                                                                                                       gACCCGGTGGTGACCTTTGCCAATAGACCTGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTcc                                            <  3:297477/149‑1 (MQ=255)
                                                                                                        aCCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCg                                           <  4:176058/149‑1 (MQ=255)
                                                                                                        aCCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCg                                           >  8:124125/1‑149 (MQ=255)
                                                                                                           cGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGa                                         >  4:117476/1‑148 (MQ=255)
                                                                                                            ggtggtGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGaaa                                       <  1:213503/149‑1 (MQ=255)
                                                                                                             gtggtgACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGaaaa                                      >  6:207751/1‑149 (MQ=255)
                                                                                                                gtgACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACAt                                   >  2:26016/1‑149 (MQ=255)
                                                                                                                     cTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTgg                              <  3:117476/149‑1 (MQ=255)
                                                                                                                           cAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGGTTTAATTCAAAGGAATGAAAAAAATGAAAATAGGCACACAGAATCAGGCGTTCTTTCCGTCAAACATTCTGGAGAAAt                        >  6:50668/1‑149 (MQ=255)
                                                                                                                           cAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAAAGGGCGTTACGATTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAAt                        >  3:164423/1‑149 (MQ=255)
                                                                                                                           cAATAGACCGGAAAGCTATATGCAGTATGTAGAACAGCATCCGTAACAGAGCATCAAAATGGGCGTTACGTTATAATTCAAAGGAATGAAAAAAATGAAAATCGGCACCCAGAATCAGGCGTCCTTTCCGGAAAACATTCTGGAGAAAt                        >  2:91720/1‑149 (MQ=255)
                                                                                                                              tAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTc                     <  8:325667/149‑1 (MQ=255)
                                                                                                                               aGCCCGGAAATCTATAAGCATTATGTCGACCATCATCCCGAACAGTGCATCAAAATTGGCGTTACGTTTTAATTCCAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCg                    <  1:40711/149‑1 (MQ=255)
                                                                                                                                gCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGATCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGt                   <  5:50668/149‑1 (MQ=255)
                                                                                                                                 cccGGAAAGCTATATGCAGTCTGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGGTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGTATCTGTCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGtt                  <  1:327170/149‑1 (MQ=255)
                                                                                                                                  ccGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTta                 <  5:207751/149‑1 (MQ=255)
                                                                                                                                    ggAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTtata               >  8:40233/1‑149 (MQ=255)
                                                                                                                                     gAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGTATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTatat              >  7:62838/1‑149 (MQ=255)
                                                                                                                                     gAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTatat              <  4:159741/149‑1 (MQ=255)
                                                                                                                                          cTATATGCAGTATGTCGACCAGCATCCGGAAGAGAGCCTCAAAATGGGCGTTTCGTTTTAATTCAAAGGACTGAAAAAAATGAACATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACTTTCTGGAGAAATTTCGTTATATCaaa          <  8:306791/148‑1 (MQ=255)
                                                                                                                                          cTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAag         <  1:26016/149‑1 (MQ=255)
                                                                                                                                                gCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAag         >  7:382666/1‑143 (MQ=255)
                                                                                                                                                gCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAag         <  8:382666/143‑1 (MQ=255)
                                                                                                                                                 cAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATggg  >  6:149112/1‑149 (MQ=255)
                                                                                                                                                 cAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATgg   <  1:171391/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCATCCGGAACAGAGCATCAAAATGGGCGTTACGTTTTAATTCAAAGGAATGAAAAAAATGAAAATCGGCACACAGAATCAGGCGTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGG  >  NC_000913/1375817‑1376110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: