Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,472 G→C 5.6% intergenic (‑332/+32) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4720GC 5.6% 356.3 / 8.7 102intergenic (‑332/+32)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base G (45/51);  new base C (2/4);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 103 total aligned reads displayed.

ACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATC  >  NC_000913/1567331‑1567620
                                                                                                                                             |                                                                                                                                                    
aCAGCGACTATCGTATGGGTTTTCATATTCCTACATTTTTATTAGGGCTTTCTGGCTGTTTAACTACGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGc                                                                                                                                               <  8:240949/149‑1 (MQ=255)
  aGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                             <  5:389572/149‑1 (MQ=255)
     gAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATATATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                          <  2:205196/149‑1 (MQ=255)
          tCGTCTGGTTTTTCATATTCATACATTTTTATTAGGGAGTTATGGCTGGTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTCACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                     <  8:230809/149‑1 (MQ=255)
          tCGTATGGTTTTTCATATTCCAACATTTTTAATAGGGAATTATGGCTGCTTAACTACGTGTGCTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                     <  6:43216/149‑1 (MQ=255)
          tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGTGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                     <  6:77117/149‑1 (MQ=255)
          tCGTATGGTCTTTCCTATTCATACAATTTTATTAGGGAGTTATGGCTGTTTAACTAAGTGCGCTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                     <  6:37860/149‑1 (MQ=255)
             tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                                   >  4:119191/1‑148 (MQ=255)
                 gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGaa                                                                                                                              <  7:28039/149‑1 (MQ=255)
                 gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGaa                                                                                                                              <  1:119953/149‑1 (MQ=255)
                  tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                                                                                                                                                   >  5:358586/1‑127 (MQ=255)
                    tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                           >  1:172946/1‑149 (MQ=255)
                       cctcttcataCATTTTTATTAGGGATTTCTGGCTCTTTAACTAAGTGTGGCTAATTTGACTTAACTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAACGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                         <  2:364097/144‑1 (MQ=255)
                       catattcataCATTTTTATTAGTGATTTATGGCTGTTTAACTAAGTGTGTTTAATTTGACTTAAGTAAGCATGATTATTAGTTGTATATTTTAAGAGTGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                         <  5:392203/148‑1 (MQ=255)
                       catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                           <  5:142696/146‑1 (MQ=255)
                       catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                           >  6:142696/1‑146 (MQ=255)
                       catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                         <  2:172946/148‑1 (MQ=255)
                         tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                      <  3:119191/149‑1 (MQ=255)
                         tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                      <  8:78018/149‑1 (MQ=255)
                         tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                      >  8:89925/1‑149 (MQ=255)
                          attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                             >  5:296346/1‑125 (MQ=255)
                          attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                             <  6:296346/125‑1 (MQ=255)
                           ttcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                                     >  6:386588/1‑148 (MQ=255)
                             cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTaa                                                                                                                   <  7:89925/148‑1 (MQ=255)
                              ttaCATTTTTATTAGGGATTTATGGCTTTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGTGTATCAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                           <  8:229384/122‑1 (MQ=255)
                              ataCATTTTTATTAGGGATTTATTGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                                  <  3:37490/148‑1 (MQ=255)
                              ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                                 >  8:316882/1‑149 (MQ=255)
                              ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCGTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                                 >  1:78984/1‑149 (MQ=255)
                                  aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                             >  3:17455/1‑149 (MQ=255)
                                  aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                             >  2:169817/1‑149 (MQ=255)
                                   tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                            <  6:303522/117‑1 (MQ=255)
                                   tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                            >  5:303522/1‑117 (MQ=255)
                                         ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACATGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGAc                                                                                                      >  2:91294/1‑149 (MQ=255)
                                         ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                          <  4:354576/129‑1 (MQ=255)
                                         ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                          >  3:354576/1‑129 (MQ=255)
                                         ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGAc                                                                                                      >  8:85332/1‑149 (MQ=255)
                                            gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                                   >  1:266217/1‑149 (MQ=255)
                                            gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                                   >  6:169389/1‑149 (MQ=255)
                                             ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                                  >  7:201275/1‑149 (MQ=255)
                                             ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                                  >  3:18351/1‑149 (MQ=255)
                                               aTTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                                <  8:201127/149‑1 (MQ=255)
                                                    tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                            <  7:85332/148‑1 (MQ=255)
                                                    tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAg                                                                                           <  4:17455/149‑1 (MQ=255)
                                                            tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGTTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTAAAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                 <  4:206650/135‑1 (MQ=255)
                                                            tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                 >  3:206650/1‑135 (MQ=255)
                                                             aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                                  >  8:10173/1‑149 (MQ=255)
                                                              aCTTAGTGTTGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGTATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                                 <  2:78984/149‑1 (MQ=255)
                                                              aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                                 >  7:2409/1‑149 (MQ=255)
                                                              aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                                 >  2:298741/1‑149 (MQ=255)
                                                              aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                                 >  2:145941/1‑149 (MQ=255)
                                                               cTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                                >  8:134288/1‑149 (MQ=255)
                                                                tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                               >  8:77844/1‑149 (MQ=255)
                                                                tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                               >  7:342718/1‑149 (MQ=255)
                                                                tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                               >  3:54611/1‑149 (MQ=255)
                                                                  aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                              <  7:316882/148‑1 (MQ=255)
                                                                  aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                              <  1:395190/148‑1 (MQ=255)
                                                                  aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                              <  5:169389/148‑1 (MQ=255)
                                                                  aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                              <  2:369656/148‑1 (MQ=255)
                                                                  aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                              <  4:18351/148‑1 (MQ=255)
                                                                   gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                            <  7:84536/149‑1 (MQ=255)
                                                                        gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                       >  2:72854/1‑149 (MQ=255)
                                                                        gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTATAAGAGGGTAACAAGCCGGTGGGTAAAGCAACGGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTCACCGCTTGTTGAGTCTg                                                                       >  3:350294/1‑149 (MQ=255)
                                                                         ttAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGc                                                                      >  7:122793/1‑149 (MQ=255)
                                                                           aaTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                    >  8:142499/1‑149 (MQ=255)
                                                                             tttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                 >  4:369677/1‑118 (MQ=255)
                                                                             tttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                 <  3:369677/118‑1 (MQ=255)
                                                                                 aCTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAtt                                                              <  5:348841/149‑1 (MQ=255)
                                                                                  cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAtt                                                              >  3:269747/1‑148 (MQ=255)
                                                                                  cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTc                                                             <  7:134288/149‑1 (MQ=255)
                                                                                     aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCa                                                            <  1:150111/147‑1 (MQ=255)
                                                                                        taagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAg                                                       >  6:85780/1‑149 (MQ=255)
                                                                                          agCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAg                                                       <  4:367054/147‑1 (MQ=255)
                                                                                                attattAGTGTGATAGTTTAAGAGGTTAAAAAGCGGGTTGGTAAAGCAAAGGGTTGTTACAAAGTAATTATGTGAGTTTTACTGCCCCTGCGACTTTGCAGATAGCTGACCGCTTGTTGTGTCTGCGGTTTATTCAGATTGTCCTCACg                                               <  4:350294/149‑1 (MQ=255)
                                                                                                attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                    <  3:287022/128‑1 (MQ=255)
                                                                                                attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                    >  4:287022/1‑128 (MQ=255)
                                                                                                    ttaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGaa                                            <  7:10173/148‑1 (MQ=255)
                                                                                                       gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCCCCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                           >  6:89955/1‑146 (MQ=255)
                                                                                                       gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                           >  8:72668/1‑146 (MQ=255)
                                                                                                          ggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                                      <  8:122793/148‑1 (MQ=255)
                                                                                                          ggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                                      <  5:386588/148‑1 (MQ=255)
                                                                                                               gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGc                                 <  2:266217/148‑1 (MQ=255)
                                                                                                                tttAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                               >  4:335623/1‑149 (MQ=255)
                                                                                                                 ttAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGc                              >  6:219266/1‑149 (MQ=255)
                                                                                                                    agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTaa                           <  1:169817/149‑1 (MQ=255)
                                                                                                                    agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTaa                           >  2:256807/1‑149 (MQ=255)
                                                                                                                       ttgTAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                                      >  4:290100/3‑135 (MQ=38)
                                                                                                                       ttgTAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                                      <  3:290100/133‑1 (MQ=38)
                                                                                                                        ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                         <  4:100806/83‑1 (MQ=255)
                                                                                                                        ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                         >  3:100806/1‑83 (MQ=255)
                                                                                                                          tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                     <  7:77844/149‑1 (MQ=255)
                                                                                                                          tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                     <  5:219266/149‑1 (MQ=255)
                                                                                                                            aCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg                    <  7:72668/148‑1 (MQ=255)
                                                                                                                             cAAGCCGGGGGGTAACGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTACCTGCCCCAGCGACTTTGCCGAGAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGca                  <  1:145941/149‑1 (MQ=255)
                                                                                                                                gCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg               >  5:20135/1‑149 (MQ=255)
                                                                                                                                      gggacaaGCCCCGGCCTGTTACAAAGACAGAATGGGACTTACACAGCCCCAGCGACTTTCCACATAGCTGACCGCGTGCCGAGTCTCCTGTTTATTCAGAAAGTCCTCACGGAACACGATGGTGCCGCTAATTTCGGGCACAGCATCGt         <  2:232893/144‑1 (MQ=255)
                                                                                                                                      gggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGt         >  4:258840/1‑149 (MQ=255)
                                                                                                                                         tAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGtt        >  1:241530/1‑147 (MQ=255)
                                                                                                                                           aaGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAg     <  3:258840/148‑1 (MQ=255)
                                                                                                                                           aaGCACCGGATTGTTAAAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCAAAGCATCGTTAAGa    <  2:379856/149‑1 (MQ=255)
                                                                                                                                             gCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATc  <  4:269747/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
ACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATC  >  NC_000913/1567331‑1567620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: