Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,867,030 G→A 5.2% R41R (CGG→CGA yeaG → protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,867,0300GA 5.2% 245.8 / 2.6 77R41R (CGG→CGAyeaGprotein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base A (2/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01

GCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGATTGAACAGATTGTCTCT  >  NC_000913/1866882‑1867177
                                                                                                                                                    |                                                                                                                                                   
gCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGCTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTACACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGAGCTTATGCCAACGCGGCTGAGCgg                                                                                                                                                     >  5:283176/1‑149 (MQ=255)
  tAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGc                                                                                                                                                    >  7:210796/1‑148 (MQ=255)
    aaaaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGTGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                                                                                                                                 <  2:59861/149‑1 (MQ=255)
    aaaaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                                                                                                                                 >  8:42973/1‑149 (MQ=255)
    aaaaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                                                                                                                                 <  8:261339/149‑1 (MQ=255)
    aaaaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAt                                                                                                                                                 <  8:210796/149‑1 (MQ=255)
     aaaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTAtt                                                                                                                                                >  6:47/1‑149 (MQ=255)
      aaaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTg                                                                                                                                               <  2:245986/149‑1 (MQ=255)
        aCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTAACACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                                                                                                                             <  2:341746/149‑1 (MQ=255)
        aCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACAATGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                                                                                                                             >  1:108544/1‑149 (MQ=255)
          gAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATgg                                                                                                                                           <  2:178235/149‑1 (MQ=255)
          gAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGATTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATgg                                                                                                                                           <  1:74764/149‑1 (MQ=255)
               gccGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGACGCTGCCAAGGCCGAAGAGTTCACACTGGAGGAGTTTCTTACCAGTTGTCGGCAAGATCGCAGTGCTTATGCCCACGCGGCTGAGCGGATATTGATGGCTAtg                                                                                                                                      >  5:283536/3‑148 (MQ=255)
               gACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGCCGAAGAGTTCACCCTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATc                                                                                                                                      >  6:305521/1‑149 (MQ=255)
                    cATAACATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGa                                                                                                                                 <  3:26068/149‑1 (MQ=255)
                      tACCATGAATATATTCGATCACTATCGCCAGCGATATGACGCTGCCAAGGACGAAGAGTGCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGc                                                                                                                               <  5:21931/149‑1 (MQ=255)
                      tACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGc                                                                                                                               <  6:228405/149‑1 (MQ=255)
                         gaTGAATATATTCAATCACTATCGCCAGCGATATGAAGGTGCCTAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                                                                                                                             <  8:265826/131‑1 (MQ=255)
                         cATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTg                                                                                                                            <  8:211394/149‑1 (MQ=255)
                         cATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTg                                                                                                                            >  7:211394/1‑149 (MQ=255)
                         cATGAATATATTCAATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGAt                                                                                                                                             >  7:265826/1‑132 (MQ=255)
                          aTGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGt                                                                                                                           >  1:367887/1‑149 (MQ=255)
                             aaTATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCa                                                                                                                         <  6:102544/148‑1 (MQ=255)
                              atatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATg                                                                                                                       <  5:47/149‑1 (MQ=255)
                               tatatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATgg                                                                                                                      >  7:306038/1‑149 (MQ=255)
                                 tatTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTc                                                                                                                    <  6:78758/149‑1 (MQ=255)
                                     cGATCACTATCGCCAGCGTTATGAAGCTGCCAAGGACGAAGAGTTCACACTGAAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCGGAGCGGCTATTGATGGCTATCGGGGAGCCTGTCATGGTCGAt                                                                                                                 <  6:283176/148‑1 (MQ=255)
                                         cACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGc                                                                                                            >  4:291740/1‑149 (MQ=255)
                                           cTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACCCTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGGGCGGCTAt                                                                                                                                                 >  5:111898/1‑110 (MQ=255)
                                           cTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGGGCGGCTAt                                                                                                                                                 <  6:111898/110‑1 (MQ=255)
                                                 ccAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAAc                                                                                                    <  3:291740/149‑1 (MQ=255)
                                                  cAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAAcc                                                                                                   >  2:348668/1‑149 (MQ=255)
                                                     cGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTc                                                                                                                    <  5:309409/129‑1 (MQ=255)
                                                     cGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTc                                                                                                                    >  6:309409/1‑129 (MQ=255)
                                                            aaGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTtct                                                                                         <  7:42973/149‑1 (MQ=255)
                                                             aGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTtctc                                                                                        <  7:26640/149‑1 (MQ=255)
                                                                 gCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGAct                                                                                    >  5:82015/1‑149 (MQ=255)
                                                                      ggACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCtttt                                                                               <  4:129116/149‑1 (MQ=255)
                                                                        aCGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCt                                                                             <  4:107072/149‑1 (MQ=255)
                                                                          gAAGAGTTCACACTGCAGGAGTTTCATACCACTTGTCGTCAAGATCGCAGTGCATATGCCAACGCCGCTCAGCGGCTAAAGATCGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACACTATTCTaa                                                                           >  3:169038/1‑149 (MQ=255)
                                                                            agagTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGCTCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGACCAGGAACCCAGACTTTCTCGACTCTTTTCTAAcc                                                                         >  6:152388/1‑149 (MQ=255)
                                                                             gagTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCg                                                                        >  7:304675/1‑149 (MQ=255)
                                                                                 tCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGt                                                                     >  3:367935/1‑148 (MQ=255)
                                                                                   acacTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTtct                                                                                         >  1:348632/1‑126 (MQ=255)
                                                                                   acacTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTtct                                                                                         <  2:348632/126‑1 (MQ=255)
                                                                                       tGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCtttt                                                                               <  1:351702/132‑1 (MQ=255)
                                                                                       tGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCtttt                                                                               >  2:351702/1‑132 (MQ=255)
                                                                                       tGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCa                                                              >  5:9216/1‑149 (MQ=255)
                                                                                               tttCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGCCTTTCTCGCCTCTTTTCTAACCGGGTCATTGCACGTTATcc                                                      >  7:384272/1‑149 (MQ=255)
                                                                                               tttCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATcc                                                      >  5:262576/1‑149 (MQ=255)
                                                                                                   ttACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGACTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCg                                                  >  8:176325/1‑149 (MQ=255)
                                                                                                   ttACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGACTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCg                                                  >  4:200876/1‑149 (MQ=255)
                                                                                                      ccACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGttt                                               <  6:82015/149‑1 (MQ=255)
                                                                                                       cACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGttt                                               >  8:114764/1‑148 (MQ=255)
                                                                                                       cACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTg                                              <  8:304675/149‑1 (MQ=255)
                                                                                                        aCTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGa                                             <  5:305521/149‑1 (MQ=255)
                                                                                                            gTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAag                                           <  1:348668/147‑1 (MQ=255)
                                                                                                             tCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAagag                                         <  2:7394/148‑1 (MQ=255)
                                                                                                               ggCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGACCCCAGACTTTCTCGACTCGTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGttt                                      >  7:337690/1‑149 (MQ=255)
                                                                                                                gCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGttt                                      >  2:114586/1‑148 (MQ=255)
                                                                                                                gCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGttt                                      >  4:266402/1‑148 (MQ=255)
                                                                                                                gCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCAGGAACCCAGACTTTCGCGAAGGTTTTCTAACCGGGTCATTGCCCGGTATACGGCCTTTGAAGAGtttt                                     >  3:316186/1‑148 (MQ=255)
                                                                                                                  aaGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTAc                                   <  1:114586/149‑1 (MQ=255)
                                                                                                                     aTCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGc                                <  8:185157/149‑1 (MQ=255)
                                                                                                                              cTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAAACGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGAc                       >  2:327149/1‑149 (MQ=255)
                                                                                                                               gtaagCCCACGCGGCTGAGCGGCTCTTGATGGCGACCGCAGAGCCGGGAAGTTTCGATACACCCCAGGAAACCAGACATTCTCGACTCTATTCAAACCGGGTCATTGCACGTTATCCGTCGTTAGAAGAGTTTTACGGCATGGAAGAcg                      <  6:283536/145‑1 (MQ=255)
                                                                                                                                  tGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGa                   <  3:183283/149‑1 (MQ=255)
                                                                                                                                   gCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAt                  >  3:237318/1‑149 (MQ=255)
                                                                                                                                    ccAACTGGGCTGAGCGGCTTTTGATTGCTATCGGTGAGCCTGTCTTGGTCTATACAGGCGATCAACCCAGACTTTCTCGACTCTTTTCTAACCGGTTCTTTGCACGTTATCCGGCGTTTGAAGAGTTTTACGTCATGGAAGAGGCTAt                  <  8:337690/148‑1 (MQ=255)
                                                                                                                                    ccAACGCGGCTGAGCGACTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAtt                 <  7:176325/149‑1 (MQ=255)
                                                                                                                                    ccAACGCGGCTGAGCGACTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAtt                 <  3:200876/149‑1 (MQ=255)
                                                                                                                                     cAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCAc                                                             >  7:91237/1‑104 (MQ=255)
                                                                                                                                     cAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCAc                                                             <  8:91237/104‑1 (MQ=255)
                                                                                                                                     cAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAtt                 >  5:243350/1‑148 (MQ=255)
                                                                                                                                           ggCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAt                  >  4:83128/1‑141 (MQ=255)
                                                                                                                                           ggCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGAt                  <  3:83128/141‑1 (MQ=255)
                                                                                                                                             cTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGGCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGTAGACGCGATTGAACAGAtt        <  2:367887/149‑1 (MQ=255)
                                                                                                                                                aGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGATTGAACAGATTGt      <  2:108544/148‑1 (MQ=255)
                                                                                                                                                   ggCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCAAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGATTGAACAGATTGTctct  >  1:306691/1‑149 (MQ=255)
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GCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGCGTTTGAAGAGTTTTACGGCATGGAAGACGCGATTGAACAGATTGTCTCT  >  NC_000913/1866882‑1867177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: