Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,925,473 G→C 5.3% E12Q (GAG→CAG)  yobB → C‑N hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,925,4730GC 5.3% 260.5 / 5.9 75E12Q (GAG→CAG) yobBC‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (33/38);  new base C (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCC  >  NC_000913/1925326‑1925616
                                                                                                                                                   |                                                                                                                                               
cAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGa                                                                                                                                                >  7:257248/1‑149 (MQ=255)
  aaCTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGc                                                                                                                                              <  2:132852/149‑1 (MQ=255)
   aCTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATCAGcc                                                                                                                                             >  3:91427/1‑149 (MQ=255)
   aCTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTGTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATCAGcc                                                                                                                                             >  3:86137/1‑149 (MQ=255)
     ttAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTc                                                                                                                                           >  7:372761/1‑149 (MQ=255)
       aaataaaACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCg                                                                                                                                          <  3:281055/148‑1 (MQ=255)
       aaataaaACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCg                                                                                                                                          >  5:282628/1‑148 (MQ=255)
         ataaaACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTaa                                                                                                                                       <  6:282628/149‑1 (MQ=255)
         ataaaACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAAACGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTa                                                                                                                                        >  5:129550/1‑148 (MQ=255)
           aaaaCTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTaaaa                                                                                                                                     >  5:377311/1‑149 (MQ=255)
            aaaCTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAAc                                                                                                                                    <  6:342410/149‑1 (MQ=255)
                      agagTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCAcc                                                                                                                          <  1:140444/149‑1 (MQ=255)
                      agagTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCAcc                                                                                                                          >  2:140444/1‑149 (MQ=255)
                      agagTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCAcc                                                                                                                          >  1:102729/1‑149 (MQ=255)
                      agagTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCAcc                                                                                                                          >  1:193636/1‑149 (MQ=255)
                          atACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATATCCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGATAGTAGCAGCAGCGCAATATGAGCCTCGTAATACCACACTCACCGaa                                                                                                                       >  2:196779/2‑148 (MQ=255)
                             cacTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAg                                                                                                                    <  4:187634/148‑1 (MQ=255)
                              acTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGt                                                                                                                  <  6:29595/149‑1 (MQ=255)
                                tttCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAg                                                                                                                >  4:378598/1‑149 (MQ=255)
                                tttCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAg                                                                                                                >  3:341197/1‑149 (MQ=255)
                                      aCATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtcat                                                                                                           >  8:401242/1‑148 (MQ=255)
                                         tAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCatcatc                                                                                                       <  7:279753/149‑1 (MQ=255)
                                             gCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGa                                                                                                   <  6:26682/149‑1 (MQ=255)
                                              ccTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAg                                                                                                  >  8:4342/1‑149 (MQ=255)
                                              ccTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTAGAAAAGGAAGTCATTATGGCGATCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAg                                                                                                  <  8:270923/149‑1 (MQ=255)
                                               cTGCTAAATTATGAGTATTTTCTAAACCGCCCTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACATCACTCACCGAACAGGTAGCTCATCATCTGGAGt                                                                                                 >  5:189281/1‑149 (MQ=255)
                                               cTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGt                                                                                                 <  1:58183/149‑1 (MQ=255)
                                                  cTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGttt                                                                                               <  8:372761/148‑1 (MQ=255)
                                                    aaaTTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTtgt                                                                                             >  1:368757/1‑148 (MQ=255)
                                                     aaTTCTGAGTTTTTTCTAAACCGCTCTGATAAATTGCAGTCATTTTGATTAGGAAGTATTTATGTCGTTCTGGAAAGTTGCAGCAGCGCTATATGAGCCTCGTAAAATCTCAATCACCGACCAGGTAGCTGCTCATCAGGAGTTtgt                                                                                             <  4:237677/147‑1 (MQ=255)
                                                     aaTTATGAGTATTTTCTTAACCGCACTCATAATTTGCAGTCATTTTGTAAAGGAAGGCATTATGTCGTTCTGGATAGTTGCAGCAGCGCAATATGAGCCTCGTAAATCCTCCCTCACCGAACAGGTAGGTCATCATCTGGAGTTtgtg                                                                                            <  1:196779/148‑1 (MQ=255)
                                                     aaTTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTtgt                                                                                             <  3:378598/147‑1 (MQ=255)
                                                     aaTTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGa                                                                                           <  8:257248/149‑1 (MQ=255)
                                                      aTTATGAGTATTTTCTAACCCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAg                                                                                          >  4:285305/1‑149 (MQ=255)
                                                       ttATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAgg                                                                                         <  6:377311/149‑1 (MQ=255)
                                                       ttATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATCAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAgg                                                                                         <  4:86137/149‑1 (MQ=255)
                                                       ttATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATCAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAgg                                                                                         <  4:91427/149‑1 (MQ=255)
                                                       cgatcaGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAg                                                                                          <  1:296601/143‑1 (MQ=255)
                                                              tATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATAATCTGGAGTTTGTGat                                                                                          >  2:296601/1‑140 (MQ=255)
                                                                 tttCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGgccgccgc                                                                                 >  7:196540/1‑147 (MQ=255)
                                                                  ttCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCa                                                                               <  7:401242/148‑1 (MQ=255)
                                                                   tCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAg                                                                              <  2:193636/148‑1 (MQ=255)
                                                                   tCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAg                                                                              <  5:307476/148‑1 (MQ=255)
                                                                        aCCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGAAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTATCTAATCATCTGGAGTTTGTGAGGGACTCCGCAAGGcaac                                                                         >  6:295493/1‑148 (MQ=255)
                                                                         ccGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaacaa                                                                       <  6:129550/149‑1 (MQ=255)
                                                                          cGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAAt                                                                      <  7:4342/149‑1 (MQ=255)
                                                                               tCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAg                                                                                                                    <  6:137710/98‑1 (MQ=255)
                                                                               tCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAg                                                                                                                    >  5:137710/1‑98 (MQ=255)
                                                                                 aTAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTTAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCt                                                               <  4:341197/149‑1 (MQ=255)
                                                                                 aTAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCt                                                               >  2:385324/1‑149 (MQ=255)
                                                                                          aGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAAc                                                                                                                                    <  6:115594/71‑1 (MQ=255)
                                                                                          aGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAAc                                                                                                                                    >  5:115594/1‑71 (MQ=255)
                                                                                            tCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTc                                                    <  8:196540/149‑1 (MQ=255)
                                                                                                   gAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCg                                                                                                                                          <  5:93661/56‑1 (MQ=255)
                                                                                                   gAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCg                                                                                                                                          >  6:93661/1‑56 (MQ=255)
                                                                                                   gAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTc                                                                   <  4:29848/127‑1 (MQ=255)
                                                                                                   gAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTc                                                                   >  3:29848/1‑127 (MQ=255)
                                                                                                              cATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGCACAGGTAGCTCATCATCTTGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTgg                                  >  3:27841/1‑149 (MQ=255)
                                                                                                              cATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCACCAATGTCAGCTGTTAGTTTTTCCTTCGCTTTCTGTACTgg                                  >  6:76899/1‑149 (MQ=255)
                                                                                                               aTTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaa                                                                          <  5:20219/108‑1 (MQ=255)
                                                                                                               aTTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaa                                                                          >  6:20219/1‑108 (MQ=255)
                                                                                                                ttATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTgggg                                >  5:384344/1‑149 (MQ=255)
                                                                                                                  aTGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGgtg                              >  5:251095/1‑149 (MQ=255)
                                                                                                                     tCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGa                           <  1:219250/149‑1 (MQ=255)
                                                                                                                           tGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCCGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTc                     >  8:379603/1‑149 (MQ=255)
                                                                                                                             gAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg                                 >  3:233840/1‑135 (MQ=255)
                                                                                                                             gAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg                                 <  4:233840/135‑1 (MQ=255)
                                                                                                                             gAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTc                     <  6:189281/147‑1 (MQ=255)
                                                                                                                                     cagcagCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGc            <  2:368757/148‑1 (MQ=255)
                                                                                                                                        cagcGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGACGCCCCCAGGCAACAATGTCAGCTGTTAGTTTTACCATCGCTTTCTTTACTGGGGTGAGATTATTCGCGACGTGCCCtt        >  5:498/1‑149 (MQ=255)
                                                                                                                                         agcGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTc       <  3:285305/149‑1 (MQ=255)
                                                                                                                                         agcGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTc       <  6:384344/149‑1 (MQ=255)
                                                                                                                                          gagcAATATGAGCCTCGTAAAACCTAACTCACCTAAGAGGTAGCTCATCATCTGGATTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc      <  5:295493/147‑1 (MQ=255)
                                                                                                                                             cAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGc   >  4:248478/1‑149 (MQ=255)
                                                                                                                                              aaTATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGcc  <  6:251095/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCC  >  NC_000913/1925326‑1925616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: