Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 235,980 A→G 8.7% F8S (TTC→TCC)  rnhA ← ribonuclease HI, degrades RNA of DNA‑RNA hybrids

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913235,9800AG 8.7% 126.2 / 3.4 46F8S (TTC→TCC) rnhAribonuclease HI, degrades RNA of DNA‑RNA hybrids
Reads supporting (aligned to +/- strand):  ref base A (18/24);  new base G (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATG  >  NC_000913/235836‑236120
                                                                                                                                                |                                                                                                                                            
aGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGTGGGGGTAGCCAGCGCTAAAGGTTTTCTCGCGGCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAAt                                                                                                                                          <  5:101060/149‑1 (MQ=255)
     gACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTa                                                                                                                                     <  7:85144/149‑1 (MQ=255)
     gACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTa                                                                                                                                     <  3:26394/149‑1 (MQ=255)
          tAGCGGCCATGCTCTCCTTACGGGTGTTGGGGGTGCGGGTGTTGCGTGGGGTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACGCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAATATTTCTACCTGt                                                                                                                                <  3:155556/149‑1 (MQ=255)
              ggCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTaa                                                                                                                            <  8:73346/149‑1 (MQ=255)
                  aTCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCAt                                                                                                                        >  2:201361/1‑149 (MQ=255)
                          cATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTgg                                                                                                                  >  5:183143/1‑147 (MQ=255)
                               ggTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACtt                                                                                                           >  6:71526/1‑149 (MQ=255)
                                gttgttGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTc                                                                                                          <  6:183143/149‑1 (MQ=255)
                                   gttgGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATATCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCt                                                                                                        <  1:201361/148‑1 (MQ=255)
                                        tggtgCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAAtt                                                                                                  <  7:89699/149‑1 (MQ=255)
                                                gtgtAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGaa                                                                                          <  1:188986/149‑1 (MQ=255)
                                                  gtAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTACCCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGAAATTGAATCGAACt                                                                                        >  5:192877/1‑149 (MQ=255)
                                                   tAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACt                                                                                        <  5:71526/148‑1 (MQ=255)
                                                    aGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTg                                                                                       >  5:72519/1‑148 (MQ=255)
                                                     gCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGt                                                                                      <  4:118264/148‑1 (MQ=255)
                                                      ccAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCCGGTCCTGGATTGCCCAGACACGAACCATCGGTGGAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCGGTAATTGAATCGCACTGTa                                                                                     >  5:29964/1‑148 (MQ=255)
                                                      ccAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGGAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTa                                                                                     >  5:33225/1‑148 (MQ=255)
                                                      ccAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTa                                                                                     >  6:81362/1‑148 (MQ=255)
                                                      ccAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTa                                                                                     >  7:28192/1‑148 (MQ=255)
                                                      ccAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTa                                                                                     >  1:228424/1‑148 (MQ=255)
                                                        aGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTaaaa                                                                                  <  2:226319/149‑1 (MQ=255)
                                                         gcgcTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAAc                                                                                 >  4:38739/1‑149 (MQ=255)
                                                              aaaGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGAc                                                                              >  3:221217/1‑147 (MQ=255)
                                                               aaGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACaa                                                                            <  7:68089/148‑1 (MQ=255)
                                                                 ggTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGATCTGTAAAACGACAAGTc                                                                         >  3:76071/1‑149 (MQ=255)
                                                                   ttttCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTg                                                                       <  3:154434/149‑1 (MQ=255)
                                                                          gcgTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTg                                                                                       <  5:51140/126‑1 (MQ=255)
                                                                          gcgTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTg                                                                                       >  6:51140/1‑126 (MQ=255)
                                                                                    tAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTAt                                                      <  6:155457/149‑1 (MQ=255)
                                                                                            aaTAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGAc                                                            >  4:41483/1‑135 (MQ=255)
                                                                                            aaTAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGAc                                                            <  3:41483/135‑1 (MQ=255)
                                                                                                gcgcCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCaa                                           <  2:228424/148‑1 (MQ=255)
                                                                                                          ccccAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGc                                   >  2:126341/1‑146 (MQ=255)
                                                                                                            ccAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGc                                   >  6:242484/1‑144 (MQ=255)
                                                                                                                   cTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGGAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTtct                        <  3:182613/148‑1 (MQ=255)
                                                                                                                      gATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa                     >  8:149191/1‑148 (MQ=255)
                                                                                                                       aTTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATa                   <  1:126341/149‑1 (MQ=255)
                                                                                                                        ttGCCCAGACACGAACCATCGGTGGAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGACAGATCGTTCTCGATAc                  <  6:33225/149‑1 (MQ=255)
                                                                                                                          gaccAGACACGAACCATCTGTGGAAATTTCTACCTGTTTACGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCGATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAc                  <  6:29964/145‑1 (MQ=255)
                                                                                                                            ccAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaa              <  5:81362/149‑1 (MQ=255)
                                                                                                                             cAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaac             >  4:200562/1‑148 (MQ=255)
                                                                                                                                     aaCCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGt     <  4:221217/149‑1 (MQ=255)
                                                                                                                                       ccATCGGTGAAAATTTCTTCCTGTTTTAGGATCTCTGGTAGACTTCCTGTATTTGAATCGAATTGTAAAACGACAAGTATGAGATAAATTAACGCTATTAGCAATTCAATTTCACGCCAGATCGTTCTGGTTACCGAAACCACCTGTAt   <  6:192877/149‑1 (MQ=255)
                                                                                                                                        cATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg  <  4:115637/149‑1 (MQ=255)
                                                                                                                                         aTCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg  >  8:111814/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                            
AGCGCGACAATAGCGGCCATCAACTCCATACGGTTGTTGGTGGTGCGGGTGTAGCCAGCGCTAAAGGTTTTCTCGCGTCCGCGATAGCGTAAAATAGCGCCGTAACCCCCAGGTCCTGGATTGCCCAGACACGAACCATCGGTGAAAATTTCTACCTGTTTAAGCATCTCTGGTAGACTTCCTGTAATTGAATCGAACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATG  >  NC_000913/235836‑236120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑