| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,844 | C→G | 30.6% | A642A (GCC→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,844 | 0 | C | G | 30.6% | 7.6 / 3.2 | 13 | A642A (GCC→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base G (2/2); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGG > NC_000913/1708772‑1708956 | gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGCGCCAGAACAACAGGTCGATCCGCCGAAAGCCGACGTCGAAGCCGCAATAGCACTAGCAAAAGCGCGCAAACgg > 8:171712/1‑148 (MQ=14) aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACATCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAg < 7:171712/149‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGAGCAAAGCAGCCGTCGAATCCGCTATTGCCCGAGCATAGGCGCGCACACGGTCAGCGCAACAGGCTaa > 7:132860/1‑149 (MQ=14) cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAAGAACAGGTCGGTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCggcg > 1:22600/1‑147 (MQ=255) cgCCAAATCACGAAACCTGGAACAGCCACACCCTACAGCCGACCCAGCACACCCTGTCGATCCCAACAACCCCGCCGTCGCAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAc < 2:22600/148‑1 (MQ=255) taaataaCGAAAGCGGGAACAGCAACATCAAAATCCCGACCCACAACAATAGCTTCAACCTCGCAAATCCGAAGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg < 8:132860/143‑1 (MQ=9) cAAAGCGCGCTAGCTGGAGCTGCATCACGCTAATGCGGTTGCTCTTCAAATGGTAGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg < 1:13006/149‑1 (MQ=11) gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa < 1:119415/38‑1 (MQ=255) gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 2:119415/1‑38 (MQ=255) aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa > 1:72659/1‑92 (MQ=255) aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa < 2:72659/92‑1 (MQ=255) tAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa < 1:87923/85‑1 (MQ=255) tAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa > 2:87923/1‑85 (MQ=255) | GCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGG > NC_000913/1708772‑1708956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |