Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,779,586 A→T 7.7% intergenic (‑285/‑31) ydiP ← / → ydiQ putative DNA‑binding transcriptional regulator/putative electron transfer flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,779,5860AT 7.7% 154.3 / 3.6 52intergenic (‑285/‑31)ydiP/ydiQputative DNA‑binding transcriptional regulator/putative electron transfer flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base A (18/30);  new base T (2/2);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

AATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGTTCGATCT  >  NC_000913/1779439‑1779729
                                                                                                                                                   |                                                                                                                                               
aaTAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGAt                                                                                                                                                <  3:178050/149‑1 (MQ=255)
    aCTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGAATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGTCCATTTTTACCTTCCATCGGAGATGGtt                                                                                                                                            <  1:94568/149‑1 (MQ=255)
        tatTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCg                                                                                                                                         >  1:155629/1‑148 (MQ=255)
              cGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGa                                                                                                                                  <  8:171237/149‑1 (MQ=255)
                   aTTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTca                                                                                                                              >  3:17646/1‑148 (MQ=255)
                    ttGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTca                                                                                                                              >  1:161703/1‑147 (MQ=255)
                      gCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAg                                                                                                                           <  5:154609/148‑1 (MQ=255)
                       cACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAgg                                                                                                                          <  7:19214/148‑1 (MQ=255)
                       cACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAg                                                                                                                           >  1:82992/1‑147 (MQ=255)
                                       aTCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAactaa                                                                                                         >  4:43797/1‑149 (MQ=255)
                                       aTCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAaataa                                                                                                         <  1:209478/149‑1 (MQ=255)
                                           cATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc                                                                                                     <  1:171102/149‑1 (MQ=255)
                                           cATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc                                                                                                     <  2:161703/149‑1 (MQ=255)
                                           cATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAc                                                                                                     <  4:132794/149‑1 (MQ=255)
                                             ttttttttGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCt                                                                                                   >  2:106004/1‑149 (MQ=255)
                                                     gCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTaa                                                                                            <  2:155629/148‑1 (MQ=255)
                                                       aaGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGTTGGTTCCGTATGCGACTCACAGGAGAAATCATGaaaa                                                                                                            >  6:156392/1‑130 (MQ=255)
                                                       aaGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGTTGGTTCCGTATGCGACTCACAGGAGAAATCATGaaaa                                                                                                            <  5:153032/130‑1 (MQ=255)
                                                       aaGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGTTGGTTCCGTATGCGACTCACAGGAGAAATCATGaaaa                                                                                                            <  5:156392/130‑1 (MQ=255)
                                                       aaGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGTTGGTTCCGTATGCGACTCACAGGAGAAATCATGaaaa                                                                                                            >  6:153032/1‑130 (MQ=255)
                                                                ggCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTg                                                                                 <  2:35538/148‑1 (MQ=255)
                                                                 gCAAGGAAACAAGCTTGCTCAGTCGAAAACCCCGCACCGCTAACGCACACTAATTTCAGGCGTTTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTTCTTTAAGCTGGTGCCTgaa                                                                               <  5:102744/149‑1 (MQ=255)
                                                                      gAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATTATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACa                                                                          <  7:188851/149‑1 (MQ=255)
                                                                       aaaaCAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGTCCATTTTTACCTTCCATCGGAGATGGTTCCTTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAg                                                                         <  1:30237/149‑1 (MQ=255)
                                                                        aaaCAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAAGCTGCTTTTAGCTTGTGCCTGAAGAACAg                                                                         >  6:11656/1‑148 (MQ=255)
                                                                        aaaCAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAg                                                                         >  8:25432/1‑148 (MQ=255)
                                                                        aaaCAGCTTGCTCCGTCGAAAACACCTCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAgg                                                                        <  6:117648/149‑1 (MQ=255)
                                                                         aaCAGCTTGCTCCGTCGAAAACCACGCACCGCTATCGCAAACTTTTTTCAGTCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGa                                                                       <  8:173318/149‑1 (MQ=255)
                                                                              cTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCa                                                                                                                  <  2:105348/101‑1 (MQ=255)
                                                                              cTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCa                                                                                                                  >  1:105348/1‑101 (MQ=255)
                                                                              cTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAATTAATAACCTGCTTTAAGTTGGTGCCTGAAGAACAGGACAttg                                                                  <  3:43797/149‑1 (MQ=255)
                                                                                 gCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACAttgtt                                                                <  2:82992/148‑1 (MQ=255)
                                                                                 gCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACAttgtt                                                                <  7:34021/148‑1 (MQ=255)
                                                                                  cTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACATTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATtgttgt                                                              <  1:106004/149‑1 (MQ=255)
                                                                                           aaaCCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAg                                                      >  5:227669/1‑148 (MQ=255)
                                                                                               cccGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATa                                                  >  3:8855/1‑148 (MQ=255)
                                                                                                 cGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATacac                                               >  6:13713/1‑149 (MQ=255)
                                                                                                    aCCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCt                                            <  8:175804/149‑1 (MQ=255)
                                                                                                     ccGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTg                                           <  1:162332/149‑1 (MQ=255)
                                                                                                           gcGCACACTATGTTCAGGCCATTTTTAGCTTCCATCTGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTc                                     <  8:220359/148‑1 (MQ=255)
                                                                                                                 acTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAAt                               >  7:73682/1‑149 (MQ=255)
                                                                                                                          aGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCtt                                                                                               >  2:178915/1‑76 (MQ=255)
                                                                                                                          aGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCtt                                                                                               <  1:178915/76‑1 (MQ=255)
                                                                                                                          aGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGa                                                     >  5:68327/1‑118 (MQ=255)
                                                                                                                          aGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGa                                                     <  6:68327/118‑1 (MQ=255)
                                                                                                                                      ccTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCa           <  8:73682/148‑1 (MQ=255)
                                                                                                                                       cTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGt         <  4:17646/149‑1 (MQ=255)
                                                                                                                                            aTCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATtgttgt                                                              <  7:201068/91‑1 (MQ=255)
                                                                                                                                            aTCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATtgttgt                                                              >  8:201068/1‑91 (MQ=255)
                                                                                                                                            aTCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGTTCGAt    >  2:94920/1‑149 (MQ=255)
                                                                                                                                             tCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGTTCGAtc   <  7:25432/149‑1 (MQ=255)
                                                                                                                                               ggAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGTTCGAtct  >  1:224485/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AATAACTATATTGCCGCAAATTGCACTTTTGTTTTAGCGATCGCATTTTTTTTGCAAGATTGTTGGCAAGGAAAACAGCTTGCTCCGTCGAAAACCCCGCACCGCTATCGCACACTATTTTCAGGCCATTTTTACCTTCCATCGGAGATGGTTCCGTATGCGACTCACAGGAGAAATCATGAAAATAATAACCTGCTTTAAGCTGGTGCCTGAAGAACAGGACATTGTTGTCACTCCAGAATACACCCTGAATTTCGACAATGCCGACGCCAAAATCAGCCAGTTCGATCT  >  NC_000913/1779439‑1779729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑