| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,860,303 | C→T | 7.4% | D344N (GAT→AAT) | ydjL ← | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,860,303 | 0 | C | T | 7.4% | 159.8 / 3.0 | 55 | D344N (GAT→AAT) | ydjL | putative Zn‑dependent NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (25/25); new base T (2/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
CATTGATGAATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTT > NC_000913/1860155‑1860450 | cATTGATGAATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATc < 7:234444/149‑1 (MQ=255)cATTGATGAATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATc < 7:208399/149‑1 (MQ=255) aTTGATGAATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATc > 4:41943/1‑148 (MQ=255) tgatgaATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGAc > 7:153122/1‑149 (MQ=255) gaATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATc > 3:30852/1‑149 (MQ=255) aaTGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATc < 7:234759/148‑1 (MQ=255) gCGGCAATCCGGTAAGCTATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCaaa < 2:71918/149‑1 (MQ=255) cGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGc > 4:170782/1‑149 (MQ=255) cGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGc < 7:223742/149‑1 (MQ=255) tCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGa < 6:190043/117‑1 (MQ=255) accacAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc > 4:177765/1‑142 (MQ=255) accacAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc < 3:177765/142‑1 (MQ=255) ccacAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCTCCATTGCGACAGGCCGATACGa < 1:35176/149‑1 (MQ=255) cacaATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAt > 6:65179/1‑149 (MQ=255) caATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGc > 3:1033/1‑149 (MQ=255) caATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGc > 3:84655/1‑149 (MQ=255) tGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATTGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt > 2:110717/1‑148 (MQ=255) tGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATTGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt > 2:107335/1‑148 (MQ=255) ggTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat > 5:217889/1‑148 (MQ=255) tttGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatcatc > 4:167261/1‑149 (MQ=255) gTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGAATCAAACCCTTCGCGCCATTGCGACAGGACGATACGATGCGTGATCATCGGt > 7:103992/1‑149 (MQ=255) ttATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGtt < 3:41943/149‑1 (MQ=255) aTCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGttt > 7:151106/1‑148 (MQ=255) tGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACt > 7:76403/1‑149 (MQ=255) ttCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATTGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGAc < 1:110717/146‑1 (MQ=255) ttCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATTGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGAc < 1:107335/146‑1 (MQ=255) cATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa < 6:35549/148‑1 (MQ=255) cGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTg < 2:182296/149‑1 (MQ=255) gTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTgg > 1:177314/1‑149 (MQ=255) ttCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTaa > 7:159229/1‑148 (MQ=255) cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATa < 4:1033/148‑1 (MQ=255) cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATa < 4:84655/148‑1 (MQ=255) aGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGc < 5:65179/149‑1 (MQ=255) ggAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGcc < 4:101586/149‑1 (MQ=255) gTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGc < 2:177314/148‑1 (MQ=255) gTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGc < 3:167261/148‑1 (MQ=255) aTTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTa > 1:221270/1‑149 (MQ=255) atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTg > 3:74785/1‑136 (MQ=255) atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTg < 4:74785/136‑1 (MQ=255) tcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCa > 2:163062/1‑149 (MQ=255) tcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCa > 7:91195/1‑149 (MQ=255) caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCAt > 1:206014/1‑149 (MQ=255) caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCAt > 3:6933/1‑149 (MQ=255) aaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGa < 8:151106/149‑1 (MQ=255) aGTCGTAAGTCATGAGCACGTGGATTGCGGTTTTATAGACCATCGCCTAAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGGGAGCATCGGTGTGACGGTGATAGCGCCGCTGGCTAATAGCCTGATATCTGTAAGACATGAg > 6:162976/1‑149 (MQ=255) tCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCa > 4:60848/1‑149 (MQ=255) tCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCa > 2:58709/1‑149 (MQ=255) tGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGTCTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGCGGAGTCATAg < 4:30852/149‑1 (MQ=255) tGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAg < 8:159229/149‑1 (MQ=255) gATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCAt > 3:126662/1‑146 (MQ=255) gATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCAt < 4:126662/146‑1 (MQ=255) tttGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCatat > 7:21928/1‑149 (MQ=255) aTTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGcc < 6:217889/148‑1 (MQ=255) gCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCaa < 3:170782/148‑1 (MQ=255) gCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCaa < 4:151240/148‑1 (MQ=255) tCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCtttt < 2:206014/149‑1 (MQ=255) tCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCtttt < 4:6933/149‑1 (MQ=255) tCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCtttt > 4:199467/1‑149 (MQ=255) | CATTGATGAATGCCAGCGGCAATCCGGTAAGCGATCCAGCCGTGCACCACAATGCGGTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAATAGCCTGATAGCGTTACGCCATGAGGTGGAGTCATAGGCCATATGCCCAATGATGCTTTT > NC_000913/1860155‑1860450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |