Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,041,860 A→T 5.1% I162I (ATA→ATT zinT → zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,041,8600AT 5.1% 246.8 / 2.8 78I162I (ATA→ATTzinTzinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (36/38);  new base T (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

ATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAG  >  NC_000913/2041714‑2042007
                                                                                                                                                  |                                                                                                                                                   
aTTGTTGCATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATAAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATAc                                                                                                                                                    >  2:190789/1‑148 (MQ=255)
  tgttgAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAAt                                                                                                                                                 >  4:227752/1‑149 (MQ=255)
    ttgAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAAtt                                                                                                                                                >  8:143476/1‑148 (MQ=255)
     tgAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAAtt                                                                                                                                                >  1:194420/1‑147 (MQ=255)
      gAATACCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTAAAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATAAACAATTTAg                                                                                                                                             >  8:225781/1‑149 (MQ=255)
       aaTTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGc                                                                                                                                            <  1:41845/149‑1 (MQ=255)
       aaTTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGc                                                                                                                                            >  7:93115/1‑149 (MQ=255)
            cATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATc                                                                                                                                        <  5:85661/148‑1 (MQ=255)
            cATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCa                                                                                                                                       <  7:239453/149‑1 (MQ=255)
             aTAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATTCAATTTAGCGATCa                                                                                                                                       >  8:35028/1‑148 (MQ=255)
             aTAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTAGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATTCAATTTAGCGATCa                                                                                                                                       >  8:38973/1‑148 (MQ=255)
             aTAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGATCGTTACTTATTCGAATCTAAAGCTCCTGAAAGCAAAGCCCCTAAATATATACAATATAGCGATCa                                                                                                                                       >  8:64982/1‑148 (MQ=255)
                       tGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcac                                                                                                                            <  1:186064/149‑1 (MQ=255)
                        gAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGcac                                                                                                                            >  5:98553/1‑148 (MQ=255)
                                 tCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAatc                                                                                                                  <  1:14028/149‑1 (MQ=255)
                                    tgtAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAatcatc                                                                                                               >  8:183673/1‑149 (MQ=255)
                                    tgtAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGAGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAatcatc                                                                                                               <  8:96266/149‑1 (MQ=255)
                                    tgtAAATATGAATACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAatcatc                                                                                                               <  8:215421/149‑1 (MQ=255)
                                         atatGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCAtt                                                                                                          <  8:128979/149‑1 (MQ=255)
                                         atatGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCAtt                                                                                                          >  2:14670/1‑149 (MQ=255)
                                            tGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTc                                                                                                       <  6:98553/149‑1 (MQ=255)
                                               ttACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTcaca                                                                                                    <  2:208551/149‑1 (MQ=255)
                                                tACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACAt                                                                                                   >  6:135350/1‑149 (MQ=255)
                                                  cGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGATCTTTACTTATTCGAATGTAAAGATCCTGAAAGCAAACCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACAttt                                                                                                 <  8:115317/149‑1 (MQ=255)
                                                     tGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAATATCCTGAAAGAAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTa                                                                                              <  7:64982/149‑1 (MQ=255)
                                                        aTACAAAATACTCTACTATAAATCAGTCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATgg                                                                                           <  7:216722/149‑1 (MQ=255)
                                                        aTACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATgg                                                                                           <  6:118764/149‑1 (MQ=255)
                                                           cAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTa                                                                                        <  2:221263/149‑1 (MQ=255)
                                                            aaaaTACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTaa                                                                                       >  1:175662/1‑149 (MQ=255)
                                                                  cTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTc                                                                                 <  3:227752/149‑1 (MQ=255)
                                                                   tCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCTCGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTca                                                                                >  5:43595/1‑149 (MQ=255)
                                                                   tCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATATTTATGGGTAATGATTca                                                                                >  5:237309/1‑149 (MQ=255)
                                                                     aCCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTca                                                                                >  4:30000/1‑147 (MQ=255)
                                                                     aCCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTca                                                                                <  6:150772/147‑1 (MQ=255)
                                                                       cTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAg                                                                             >  8:53015/1‑148 (MQ=255)
                                                                        tataAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGc                                                                            >  8:93129/1‑148 (MQ=255)
                                                                        tataAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGc                                                                            >  6:110674/1‑148 (MQ=255)
                                                                         ataAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCaa                                                                          >  6:116239/1‑149 (MQ=255)
                                                                          taAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAAt                                                                         >  1:172651/1‑149 (MQ=255)
                                                                            aaTCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATg                                                                                            >  8:126462/1‑128 (MQ=255)
                                                                            aaTCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATg                                                                                            <  7:126462/128‑1 (MQ=255)
                                                                            aaTCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGAACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCt                                                                       >  5:152581/1‑149 (MQ=255)
                                                                             aTCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCtt                                                                      >  4:226381/1‑149 (MQ=255)
                                                                             aTCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCtt                                                                      <  7:183673/149‑1 (MQ=255)
                                                                              tCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCtt                                                                      >  1:57585/1‑148 (MQ=255)
                                                                                aGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTttat                                                                   <  5:135350/149‑1 (MQ=255)
                                                                                    aaGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTAt                                                                                             >  6:163925/1‑119 (MQ=255)
                                                                                    aaGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTAt                                                                                             <  5:163925/119‑1 (MQ=255)
                                                                                    aaGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaa                                                               >  2:246148/1‑149 (MQ=255)
                                                                                     aGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaat                                                              <  8:42645/149‑1 (MQ=255)
                                                                                         aGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCACAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaatgaaa                                                          <  2:245206/149‑1 (MQ=255)
                                                                                         aGGCGTTCGTTACTTATGAGAAAGTAAAGATCCTGCACGCACAGCCCCTAAATATCTACAATTTCGCGCTCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaatgaaa                                                          <  6:132265/149‑1 (MQ=255)
                                                                                           gCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaatgaaatg                                                        <  6:237309/149‑1 (MQ=255)
                                                                                                     cTTATTCGAATGTAAATATCCTTAAATCAAATCCCCTAAATATATTAAATTTATCTATCATATAATTGCAACAAGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGc                                              <  7:38973/149‑1 (MQ=255)
                                                                                                     cTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATTCAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGc                                              <  7:35028/149‑1 (MQ=255)
                                                                                                        aTTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGcc                                             <  7:25020/147‑1 (MQ=255)
                                                                                                        aTTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCaa                                           >  7:114440/1‑149 (MQ=255)
                                                                                                         ttCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAAc                                          <  7:93129/149‑1 (MQ=255)
                                                                                                            gAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGta                                       >  7:126859/1‑149 (MQ=255)
                                                                                                              aTGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaat                                                              >  6:178447/1‑124 (MQ=255)
                                                                                                              aTGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTaaat                                                              <  5:178447/124‑1 (MQ=255)
                                                                                                               tGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAAc                                          <  7:193746/143‑1 (MQ=255)
                                                                                                               tGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAAc                                          >  8:193746/1‑143 (MQ=255)
                                                                                                                gTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGtattat                                   >  7:71745/1‑149 (MQ=255)
                                                                                                                 tAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGTAAAACTGGCCAACGTATTATc                                  <  1:246148/149‑1 (MQ=255)
                                                                                                                    aGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCa                                >  4:46080/1‑148 (MQ=255)
                                                                                                                     gATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCata                              <  7:143476/149‑1 (MQ=255)
                                                                                                                         cTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAg                          >  3:233060/1‑149 (MQ=255)
                                                                                                                          tGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGt                         >  5:92385/1‑149 (MQ=255)
                                                                                                                            aaagcaaagcCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGtt                        <  2:194420/148‑1 (MQ=255)
                                                                                                                            aaagcaaagcCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGtt                        <  3:46080/148‑1 (MQ=255)
                                                                                                                            aaagcaaagcCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGtt                        <  2:57585/148‑1 (MQ=255)
                                                                                                                             aagcaaagcCCCTAAATATATACAATTTATCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGa                      <  1:190789/149‑1 (MQ=255)
                                                                                                                                    gcCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTa                                                                                              <  6:242036/70‑1 (MQ=255)
                                                                                                                                    gcCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTa                                                                                              >  5:242036/1‑70 (MQ=255)
                                                                                                                                       ccTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATcc                                 <  5:36568/128‑1 (MQ=255)
                                                                                                                                       ccTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATcc                                 >  6:36568/1‑128 (MQ=255)
                                                                                                                                        cTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCgaagaa           <  8:93115/149‑1 (MQ=255)
                                                                                                                                                ataCAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTc     >  6:128651/1‑147 (MQ=255)
                                                                                                                                                 taCAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAg  <  3:226381/149‑1 (MQ=255)
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ATTGTTGAATTCCATAGAAATAATGAAACAACATCGTGTAAATATGATTACGATGGATACAAAATACTCACCTATAAATCAGGCAAGAAAGGCGTTCGTTACTTATTCGAATGTAAAGATCCTGAAAGCAAAGCCCCTAAATATATACAATTTAGCGATCATATAATTGCACCACGAAAATCATCTCATTTTCACATTTTTATGGGTAATGATTCACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAG  >  NC_000913/2041714‑2042007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: