Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,068,408 T→A 8.3% pseudogene (12/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,4080TA 8.3% 197.5 / 4.8 65pseudogene (12/159 nt)yoeHpseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (35/24);  new base A (2/4);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068262‑2068554
                                                                                                                                                  |                                                                                                                                                  
ctgatactgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGt                                                                                                                                                  >  3:12538/1‑149 (MQ=255)
  gatactgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTc                                                                                                                                                 >  4:157031/1‑148 (MQ=255)
            tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCGCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                      <  3:47209/149‑1 (MQ=255)
            tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                      <  3:108243/149‑1 (MQ=255)
              aGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGTc                                                                                                                                     <  5:144898/148‑1 (MQ=39)
                tCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGt                                                                                                                                  >  4:166377/1‑149 (MQ=255)
                  ttACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTg                                                                                                                                 >  1:30704/1‑148 (MQ=255)
                      cGCATATACGGTCAATTTCAGCAATTAATTACATTATCAACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCAGTTTTCCACGGACCGCACTCTTAAGTCAAGAGTGCGGTCCGTGGATa                                                                                                                             >  5:84957/1‑148 (MQ=255)
                       gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAc                                                                                                                            >  4:158912/1‑148 (MQ=255)
                       gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGCCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATAc                                                                                                                            >  1:225999/1‑148 (MQ=255)
                        cATATACGGTCAATTTCAGCAATTAATTACATTATCCACCCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTAATGTCAAGAGTGCGGTCCGTGGATACaa                                                                                                                          <  1:49827/149‑1 (MQ=255)
                          tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGCCATCACAATGATGGTACCTTCCTTCAGACACCATTTTTTCAACTCCGTTTTTCACGGACCGCACTCTTGACATAAGAGTGCGGTCCGTGGaaaacgga                                                                                                                        <  1:231802/149‑8 (MQ=37)
                                gTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGAc                                                                                                                  >  1:146651/1‑149 (MQ=255)
                                     tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                             <  4:64862/149‑1 (MQ=255)
                                           cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                          <  1:119269/114‑1 (MQ=255)
                                           cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                          <  1:115981/114‑1 (MQ=255)
                                           cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                          >  2:119269/1‑114 (MQ=255)
                                           cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTg                                                                                                                                          >  2:115981/1‑114 (MQ=255)
                                            aattaattACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                        >  1:183268/1‑147 (MQ=255)
                                              ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGt                                                                                                    <  7:54707/149‑1 (MQ=255)
                                              ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGt                                                                                                    <  7:51247/149‑1 (MQ=255)
                                              ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCAACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGAAACAACCAGACACCGACTGACACCAGt                                                                                                    >  7:251239/1‑149 (MQ=255)
                                                 attaCATTATCCACGCCAAACTATTTGGCATCACAATGATTGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCa                                                                                                  <  6:210556/148‑1 (MQ=255)
                                                 attaCATTATCAACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAAGTCAAGAGTGCGGTCCGTGGATAAAAACAGATACCCACTGACAAGAGTCa                                                                                                  >  4:69477/1‑148 (MQ=255)
                                                  ttaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                                <  3:158912/149‑1 (MQ=255)
                                                    aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCagag                                                                                               <  2:30704/148‑1 (MQ=255)
                                                     cATTATCCACGCCAAAGTATTTGTCATAACAATGATGGTACCTTCTTTCAGCCACCATTTTTTCAACTCCGTTTTCCAAGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAgg                                                                                              >  1:19874/1‑148 (MQ=255)
                                                        tATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAac                                                                                          <  6:86962/149‑1 (MQ=255)
                                                          tCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacga                                                                                        <  3:166377/149‑1 (MQ=255)
                                                             aCGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCAACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAacgacg                                                                                      >  7:217018/1‑148 (MQ=255)
                                                                 cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGatat                                                                                 >  7:231383/1‑149 (MQ=255)
                                                                 cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGatat                                                                                 >  4:215561/1‑149 (MQ=255)
                                                                    aGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGtt                                                                              <  2:146651/149‑1 (MQ=255)
                                                                          tGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTc                                                                         <  3:215561/148‑1 (MQ=255)
                                                                             catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGt                                                                                                    >  4:27382/1‑118 (MQ=255)
                                                                             catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGt                                                                                                    <  3:27382/118‑1 (MQ=255)
                                                                             catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAgg                                                                                              <  6:233229/124‑1 (MQ=255)
                                                                             catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAgg                                                                                              >  5:233229/1‑124 (MQ=255)
                                                                             catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGAGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTa                                                                      >  4:163408/1‑148 (MQ=255)
                                                                              atcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaa                                                                     >  1:146620/1‑148 (MQ=255)
                                                                                cacaATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGac                                                                                                          >  1:200742/1‑109 (MQ=255)
                                                                                cacaATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGac                                                                                                          <  2:200742/109‑1 (MQ=255)
                                                                                 acaATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAat                                                                  <  7:26606/148‑1 (MQ=255)
                                                                                     tgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatcta                                                             >  3:97420/1‑149 (MQ=255)
                                                                                     tgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACAAGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatcta                                                             >  2:226364/1‑149 (MQ=255)
                                                                                            aCCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCaaaa                                                       >  4:43015/1‑148 (MQ=255)
                                                                                              cttcttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGATTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCaaaaaa                                                     >  5:79803/1‑148 (MQ=255)
                                                                                               ttcttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACa                                                   >  8:152242/1‑149 (MQ=255)
                                                                                                       gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAg                                           <  2:183268/149‑1 (MQ=255)
                                                                                                          acCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                        <  8:164865/149‑1 (MQ=255)
                                                                                                            cATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGGCTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCa                                       >  5:143581/1‑148 (MQ=255)
                                                                                                            cATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAg                                                                           >  1:9162/1‑112 (MQ=255)
                                                                                                            cATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAg                                                                           <  2:9162/112‑1 (MQ=255)
                                                                                                             aTTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAATATATGTATCAAAAAACATGATTAAGGTCaa                                      >  2:129299/1‑148 (MQ=255)
                                                                                                             aTTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCaa                                      >  1:138426/1‑148 (MQ=255)
                                                                                                                   tCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGtt                               >  8:230858/1‑149 (MQ=255)
                                                                                                                      aCTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGa                            >  2:249103/1‑149 (MQ=255)
                                                                                                                        tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGCTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGata                          >  8:183949/1‑149 (MQ=255)
                                                                                                                                      aCCGCAATCTTATGTCATGAGTGCGGTCCGTGGATACAACCAGAGACCGTCTGACTCGAGTCAGATTAAACGACGGATATGTTCTGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGa            <  6:84957/149‑1 (MQ=255)
                                                                                                                                        cGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATg             >  6:8259/1‑146 (MQ=255)
                                                                                                                                            ctctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGa      <  3:43015/149‑1 (MQ=255)
                                                                                                                                            ctctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGa      <  5:8259/149‑1 (MQ=255)
                                                                                                                                             tctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGa      >  4:53857/1‑148 (MQ=255)
                                                                                                                                               ttATGTCAAGAGTGCGGTCCGTGGATACCACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGATCAGTAGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAAAAATTATGAAGATGACa    >  2:208454/1‑148 (MQ=255)
                                                                                                                                                 aTGTCAAGATTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGACACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATTAAGATGACaat  <  2:19874/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068262‑2068554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: