Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,187,271 C→T 6.1% Y104Y (TAC→TAT)  rcnB → periplasmic modulator of Ni and Co efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,187,2710CT 6.1% 204.0 / 2.5 65Y104Y (TAC→TAT) rcnBperiplasmic modulator of Ni and Co efflux
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base T (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

ACCGTACCGATGAGTACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTC  >  NC_000913/2187135‑2187415
                                                                                                                                        |                                                                                                                                                
accgtaccgATGAGTACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGAtt                                                                                                                                      >  5:71028/1‑149 (MQ=255)
   gtaccgATGAGTACAACTTTAAGGAGTTGCAGTTGCGTATCCTGCCCGCGCCTGATGCCGGGACGCTCTGGACCTATATGGGTGGCGCGTTCGTTTTGATCAGCGACACCGACGGTAAAATCATTATATCCTACGAGGGTGAGAttttt                                                                                                                                   <  4:66876/149‑1 (MQ=255)
    taccgATGAGTACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGAtttttt                                                                                                                                  <  5:108176/149‑1 (MQ=255)
             gTACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGc                                                                                                                          >  4:215427/1‑148 (MQ=255)
              tACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCt                                                                                                                         >  4:79380/1‑148 (MQ=255)
              tACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCt                                                                                                                         >  4:114890/1‑148 (MQ=255)
                 aacaTTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTaaa                                                                                                                      >  7:240170/1‑148 (MQ=255)
                         gCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTATGACGGTGAGAtttttt                                                                                                                                  >  2:243260/1‑128 (MQ=255)
                         gCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTATGACGGTGAGAtttttt                                                                                                                                  <  1:239845/128‑1 (MQ=255)
                         gCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTATGACGGTGAGAtttttt                                                                                                                                  <  1:243260/128‑1 (MQ=255)
                         gCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTATGACGGTGAGAtttttt                                                                                                                                  >  2:239845/1‑128 (MQ=255)
                             tGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGACCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGc                                                                                                                <  4:68797/142‑1 (MQ=255)
                             tGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGACCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGc                                                                                                                >  3:68797/1‑142 (MQ=255)
                               gCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCtcatc                                                                                                       <  3:79380/149‑1 (MQ=255)
                                 aGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTcatcat                                                                                                     >  7:95386/1‑149 (MQ=255)
                                   ttGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGa                                                                                                   <  3:138446/149‑1 (MQ=255)
                                          aCCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGa                                                                                             >  2:234337/1‑148 (MQ=255)
                                          aCCTGCCCGCGCCAGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTCCGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggc                                                                                             <  8:99575/148‑2 (MQ=255)
                                            cTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAAt                                                                                          >  2:133610/1‑149 (MQ=255)
                                               cccGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGc                                                                                        <  6:71028/148‑1 (MQ=255)
                                               cccGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCa                                                                                       <  7:192053/149‑1 (MQ=255)
                                               cccGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCa                                                                                       <  7:119368/149‑1 (MQ=255)
                                                ccGCGCCTGATGCCGGGACGCACTGGACCGATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGCGCTGTTTTATCATCGCTACAAAAAGCCCCCTCATCATGAGGGGGAAATg                                                                                         >  5:97701/1‑146 (MQ=255)
                                                 cgcgCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGa                                                                                     <  3:215427/149‑1 (MQ=255)
                                                  gcgcCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGac                                                                                    >  5:88221/1‑149 (MQ=255)
                                                         aTGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGGGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCtt                                                                               <  1:133610/147‑1 (MQ=255)
                                                             cGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtt                                                                         >  2:217192/1‑149 (MQ=255)
                                                               ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggct                                                                                            >  4:121734/1‑126 (MQ=255)
                                                               ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggct                                                                                            <  3:121734/128‑3 (MQ=255)
                                                                         gACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatta                                                              <  8:240170/148‑1 (MQ=255)
                                                                            cTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgg                                                                                                 <  3:108061/110‑1 (MQ=255)
                                                                            cTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgg                                                                                                 >  4:108061/1‑110 (MQ=255)
                                                                               tatGGGTGGCGCGTACGTGTTGATCAGTGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACtt                                                       >  8:72372/1‑149 (MQ=255)
                                                                                  gggTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGc                                                     <  8:95386/148‑1 (MQ=255)
                                                                                    gTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCg                                                  >  6:27499/1‑149 (MQ=255)
                                                                                        cgcgTACGTGTTGATCAGCGACACAGACGGTATAATCATTAAAGCCTACGACTGTTAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTctt                                              <  7:233650/149‑1 (MQ=255)
                                                                                         gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg                                             >  1:238609/1‑149 (MQ=255)
                                                                                         gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttg                                             >  7:132845/1‑149 (MQ=255)
                                                                                                tgtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAATCCCCCTCATCATGAGGGGGTAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGtta                                      <  3:114890/149‑1 (MQ=255)
                                                                                                 gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTtta                                                                    <  5:125891/118‑1 (MQ=255)
                                                                                                 gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTtta                                                                    >  6:125891/1‑118 (MQ=255)
                                                                                                 gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttat                                     <  2:52848/149‑1 (MQ=255)
                                                                                                 gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttat                                     >  4:86839/1‑149 (MQ=255)
                                                                                                 gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttat                                     >  5:54207/1‑149 (MQ=255)
                                                                                                    gATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatta                                   <  1:217192/148‑1 (MQ=255)
                                                                                                     ctCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                  <  6:127593/147‑1 (MQ=255)
                                                                                                     aTCAGCTAAACCGACCGTAAAATCATTAACGCCTTCGACGCTGAGATTTTTTATCATCTCTAAAAAAAGCCCCCTCCTCATGAGGGGTAAATGCAGACACCTTGTTATTTTATATTATTAGCCACATGCTCGTCTTGCTTGTTATTAg                                  <  6:124418/148‑1 (MQ=255)
                                                                                                     aTCAGCGACACCGAAGGCAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGACCCCTCATCATGAGGGGGAAATGGAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAg                                  <  8:122406/148‑1 (MQ=255)
                                                                                                      tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACCCCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                >  2:192005/1‑149 (MQ=255)
                                                                                                      tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatta                                                              >  2:167987/1‑119 (MQ=255)
                                                                                                      tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatta                                                              <  1:167987/119‑1 (MQ=255)
                                                                                                          cGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTAt                            >  1:201911/1‑149 (MQ=255)
                                                                                                             cacCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTc                         <  2:238609/149‑1 (MQ=255)
                                                                                                                cGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCAc                                                         <  8:251841/114‑1 (MQ=255)
                                                                                                                cGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCAc                                                         >  7:251841/1‑114 (MQ=255)
                                                                                                                cGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACg                      >  8:61380/1‑149 (MQ=255)
                                                                                                                 gACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGt                     >  4:4182/1‑149 (MQ=255)
                                                                                                                      tAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGAtt                <  2:201911/149‑1 (MQ=255)
                                                                                                                      tAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGAt                 >  1:237044/1‑148 (MQ=255)
                                                                                                                         aaTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAAt             >  4:30208/1‑149 (MQ=255)
                                                                                                                                aaaGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTg      >  5:22605/1‑149 (MQ=255)
                                                                                                                                  aGCCTACGCCGGTGCGATTTTTTAGCATCGCTAACAAACGCCCCCTCATCCTGCTGGGGAAATGCAGACACCGTGTTCTTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTCGGCGTATTTCACGTTGATTAATGCGGTTGcc    <  6:97701/149‑1 (MQ=255)
                                                                                                                                     cTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTc  <  8:132845/148‑1 (MQ=255)
                                                                                                                                      tACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGacac                                                                                  >  5:48137/1‑67 (MQ=255)
                                                                                                                                      tACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGacac                                                                                  <  6:48137/67‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                
ACCGTACCGATGAGTACAACATTAAGCAGTGGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTC  >  NC_000913/2187135‑2187415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑