Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,281,441 A→G 9.7% G270G (GGA→GGG)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,4410AG 9.7% 112.3 / 3.5 41G270G (GGA→GGG) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (18/19);  new base G (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCCCCGCACGTC  >  NC_000913/2281295‑2281588
                                                                                                                                                  |                                                                                                                                                   
ttCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTCTGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGa                                                                                                                                                     <  3:25784/147‑1 (MQ=255)
ttCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGAt                                                                                                                                                    >  7:72279/1‑148 (MQ=255)
                  aaCCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGa                                                                                                                                    <  6:2957/146‑1 (MQ=255)
                    ccGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATgcag                                                                                                                               >  3:85337/1‑149 (MQ=255)
                           cTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGGTTACTGCCTGCCGAAGATGCAGCACTGAt                                                                                                                        >  6:80410/1‑149 (MQ=255)
                           cTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGGTTACTGCCTGCCGAAGATGCAGCACTGAt                                                                                                                        >  6:84193/1‑149 (MQ=255)
                             gAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGCGATTGTGGGAGTACTGCCTGCCGAAGATGCAGCACTGATTa                                                                                                                      >  5:204867/1‑149 (MQ=255)
                               aaaaaCAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTAc                                                                                                                     <  8:127513/148‑1 (MQ=255)
                                  aacaacaacGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAAAGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGc                                                                                                                 <  1:9670/149‑1 (MQ=255)
                                                aCCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGgcg                                                                                                    <  4:224298/148‑1 (MQ=255)
                                                           catcaGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGAtg                                                                                        >  3:82953/1‑149 (MQ=255)
                                                               aGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGtt                                                                                    <  3:9006/149‑1 (MQ=255)
                                                               aGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGtt                                                                                    <  6:221864/149‑1 (MQ=255)
                                                                 ccAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTaa                                                                                  >  3:185416/1‑149 (MQ=255)
                                                                             gTTTGATGCAACGCGCAATGGGGTTATGATTTTTGCCGCGACCGTTGAACACGCAAAAGAGATTGTGGGATTAGTGGCTGCCGAAGATGCAGCACTGATTACTGGCGTCACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAATtttt                                                                       <  5:18075/148‑1 (MQ=255)
                                                                                    tcAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCa                                                               <  5:37751/148‑1 (MQ=255)
                                                                                      aaCGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGGTTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGc                                                             <  5:80410/149‑1 (MQ=255)
                                                                                      aaCGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGGTTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGc                                                             <  5:84193/149‑1 (MQ=255)
                                                                                      aaCGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCa                                                               >  6:37751/1‑147 (MQ=255)
                                                                                                ggggTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTa                                                   >  1:227319/1‑149 (MQ=255)
                                                                                                     gatgatTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTgg                                              >  2:95467/1‑149 (MQ=255)
                                                                                                      atgatTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGt                                             >  7:168956/1‑149 (MQ=255)
                                                                                                      atgatTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGt                                             >  2:139384/1‑149 (MQ=255)
                                                                                                               gCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTcg                                     <  8:72279/148‑1 (MQ=255)
                                                                                                                ccGCGACGGGTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTcgcg                                   <  3:204492/149‑1 (MQ=255)
                                                                                                                  gcgACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCgg                                  >  6:145755/1‑148 (MQ=255)
                                                                                                                   cgACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTa                                >  4:49759/1‑149 (MQ=255)
                                                                                                                     aCGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACt                              <  3:49759/149‑1 (MQ=255)
                                                                                                                      cGGTTGCACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCCGCGTTTTCGCTATCTGGTCAACGTCGCGGTACt                              >  2:161759/1‑148 (MQ=255)
                                                                                                                                  cAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGAttt                 <  2:227319/149‑1 (MQ=255)
                                                                                                                                   aaaaGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTg                >  1:209907/1‑149 (MQ=255)
                                                                                                                                    aaaGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGa               >  6:174275/1‑149 (MQ=255)
                                                                                                                                     aaGAGATTGTGTGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCATTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGAc              <  6:204867/149‑1 (MQ=255)
                                                                                                                                      agagATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACg             <  8:168956/149‑1 (MQ=255)
                                                                                                                                       gagaTTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGc            <  4:82953/149‑1 (MQ=255)
                                                                                                                                          aTTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAg                                                              <  3:185992/96‑1 (MQ=255)
                                                                                                                                          aTTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAg                                                              >  4:185992/1‑96 (MQ=255)
                                                                                                                                              tgGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGAtt                  >  7:110772/1‑136 (MQ=255)
                                                                                                                                              tgGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGAtt                  <  8:110772/136‑1 (MQ=255)
                                                                                                                                                ggATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGacca                         >  3:215315/1‑127 (MQ=255)
                                                                                                                                                ggATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGacca                         <  4:215315/127‑1 (MQ=255)
                                                                                                                                                 gATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCCCCGCACGTc  <  4:85337/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCAGCGAAGCCGATCTCAACCGTGAGCTGAAAAAACAACAACGTATTACCCCGCACATCATCAGCCAGATTATGGAGTTTGCTGCAACGCGCAAAGGGGTGATGATTTTTGCCGCGACGGTTGAACACGCAAAAGAGATTGTGGGATTACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCCCCGCACGTC  >  NC_000913/2281295‑2281588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑