Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,313,153 G→A 5.4% noncoding (70/93 nt) micF → sRNA antisense regulator affecting ompF expression, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,313,1530GA 5.4% 232.7 / 2.3 75noncoding (70/93 nt)micFsRNA antisense regulator affecting ompF expression, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base A (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

AATCAATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATT  >  NC_000913/2313006‑2313300
                                                                                                                                                   |                                                                                                                                                   
aaTCAATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGa                                                                                                                                                    <  8:103950/149‑1 (MQ=255)
 aTCAATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGAt                                                                                                                                                   <  3:18572/149‑1 (MQ=255)
      tAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTCTCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTc                                                                                                                                              <  5:111999/149‑1 (MQ=255)
          ttATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCAt                                                                                                                                          >  5:196198/1‑149 (MQ=255)
          ttATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGcgaggcat                                                                                                                                          >  1:72628/1‑142 (MQ=255)
            aTTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCAtt                                                                                                                                         <  3:126674/148‑1 (MQ=255)
            aTTCTTAAGTATATGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCAtt                                                                                                                                         <  8:214733/148‑1 (MQ=255)
                ttAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtt                                                                                                                                    >  7:77295/1‑149 (MQ=255)
                  aaGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGtt                                                                                                                                    <  5:91144/147‑1 (MQ=255)
                  aaGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGtt                                                                                                                                    >  6:91144/1‑147 (MQ=255)
                  aaGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGAATGCGAGGCATCCGGt                                                                                                                                     <  5:231782/146‑1 (MQ=255)
                   aGTATTTGACAGGACTGAATGTTAAAACATATCCTTCACTCGCTACTAGTATAACTCCGTCTATGATCATTATCTTTATTTATTTGCGTCATTCATTTCTGTATGTCTGTTTACCCCTATTTCTACCGGATGGCTCGCATTCGGttttt                                                                                                                                 <  2:231312/149‑1 (MQ=255)
                    gTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATATCTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGtttttt                                                                                                                                <  4:187326/149‑1 (MQ=255)
                     tATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGttttttt                                                                                                                               >  3:18352/1‑149 (MQ=255)
                         tGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTAc                                                                                                                            <  3:24902/148‑1 (MQ=255)
                         ataCAGCACTGAATGTCAAAACAAACCCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATAACCTTTATTTATTACCGCCATTCATTTCTGAATGTCTGTTTACCACTATTTCAACCTTATGCCTCGCATTCGGTTTTTTTTAc                                                                                                                            <  4:147315/146‑1 (MQ=255)
                          gccAGCACTGAATGTCAAAACAAAACCTTACATCGCAACTAGAATAACTCCCGCTATCATCATAAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTAcc                                                                                                                           <  4:28714/146‑1 (MQ=255)
                               cACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCcttctt                                                                                                                     <  6:196198/149‑1 (MQ=255)
                                 cTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTa                                                                                                                   >  5:216495/1‑149 (MQ=255)
                                  tGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTa                                                                                                                   >  2:48623/1‑148 (MQ=255)
                                    aaTGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTaca                                                                                                                 >  2:98730/1‑148 (MQ=255)
                                          aaaacaaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACACTTCTTTACACACtttt                                                                                                          >  3:50537/1‑149 (MQ=255)
                                               aaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                     >  8:117410/1‑149 (MQ=255)
                                               aaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                     >  3:222712/1‑149 (MQ=255)
                                               aaaacCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                     <  2:48806/149‑1 (MQ=255)
                                                  cccTTCACTCCCAACTAGAATAACTCCCGCTATCATCAGTAACTTTATTTATTACCGGCATTCATTTCTGAATTTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTc                                                                                                  <  4:50537/148‑1 (MQ=255)
                                                    cTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                     >  2:236161/1‑144 (MQ=255)
                                                    cTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCatta                                                                                                     <  1:236161/144‑1 (MQ=255)
                                                    cTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCtg                                                                                                <  4:33727/149‑1 (MQ=255)
                                                        aCTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTAATTATTACCGTAATTCAATTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGACTCGCATTCGGTTTTTTTTACCCTTATTTACACACTTTTCATTATTCTGTGc                                                                                             >  4:67786/1‑148 (MQ=255)
                                                         cTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGACTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTa                                                                                           >  5:175722/1‑149 (MQ=255)
                                                            gCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAcc                                                                                         <  6:216495/148‑1 (MQ=255)
                                                            gCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAc                                                                                          <  5:63717/147‑1 (MQ=255)
                                                             cAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACcac                                                                                       <  2:154176/149‑1 (MQ=255)
                                                              aaCTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACca                                                                                        >  6:86901/1‑147 (MQ=255)
                                                              aaCTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACca                                                                                        >  1:104614/1‑147 (MQ=255)
                                                              aaCTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACca                                                                                        >  4:200655/1‑147 (MQ=255)
                                                                 tAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGa                                                                                    >  3:126830/1‑148 (MQ=255)
                                                                 tAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGa                                                                                    >  8:134233/1‑148 (MQ=255)
                                                                     aTAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAAc                                                                               >  8:136255/1‑149 (MQ=255)
                                                                        aCTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACta                                                                             <  1:48623/148‑1 (MQ=255)
                                                                          tCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACtat                                                                            >  1:45105/1‑147 (MQ=255)
                                                                          tCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACtat                                                                            <  2:45105/147‑1 (MQ=255)
                                                                             cGCTATCAGCATTACCTTTATTTATTACCGGCATACATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGGTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGc                                                                       <  6:178859/149‑1 (MQ=255)
                                                                                  tcatcaTTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTg                                                                    <  7:134233/147‑1 (MQ=255)
                                                                                  tcatcaTTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt                                                                   <  3:26253/148‑1 (MQ=255)
                                                                                    atcatTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTaa                                                                <  2:104614/149‑1 (MQ=255)
                                                                                      catTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAAc                                                               >  2:97291/1‑148 (MQ=255)
                                                                                       atTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTa                                                             >  7:187858/1‑149 (MQ=255)
                                                                                        ttAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTAt                                                            <  5:86901/149‑1 (MQ=255)
                                                                                           aCTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTc                                                         <  5:185762/149‑1 (MQ=255)
                                                                                            cTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTca                                                        <  7:117410/149‑1 (MQ=255)
                                                                                              ttatttatTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCACGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTcac                                                       >  8:145355/1‑148 (MQ=255)
                                                                                                        ccGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTaa                                             <  8:77295/148‑1 (MQ=255)
                                                                                                         cGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACa                                           >  7:187588/1‑149 (MQ=255)
                                                                                                                 attTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCAt                                    >  3:49041/1‑148 (MQ=255)
                                                                                                                  tttCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATac                                  <  7:175026/149‑2 (MQ=255)
                                                                                                                  tttCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATaa                                  >  8:175026/1‑149 (MQ=255)
                                                                                                                     cTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAAttt                               >  6:227170/1‑149 (MQ=255)
                                                                                                                        aaTGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTc                              <  6:175722/147‑1 (MQ=255)
                                                                                                                        aaTGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCa                            <  7:136255/149‑1 (MQ=255)
                                                                                                                         aTGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCa                            <  3:200655/148‑1 (MQ=255)
                                                                                                                          tGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGc                          <  4:222712/149‑1 (MQ=255)
                                                                                                                           gTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGc                          >  5:101386/1‑148 (MQ=255)
                                                                                                                                    ccccTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGt                 <  4:126830/148‑1 (MQ=255)
                                                                                                                                     cccTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCttt                   >  3:46053/1‑145 (MQ=255)
                                                                                                                                     cccTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCttt                   <  4:46053/145‑1 (MQ=255)
                                                                                                                                       cTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTAt             >  1:126036/1‑149 (MQ=255)
                                                                                                                                         aTTTCAACCTGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTc                              <  2:222449/130‑1 (MQ=255)
                                                                                                                                         aTTTCAACCTGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTc                              >  1:222449/1‑130 (MQ=255)
                                                                                                                                              aaCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCaa       <  1:98730/148‑1 (MQ=255)
                                                                                                                                              aaCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCaa       <  1:97291/148‑1 (MQ=255)
                                                                                                                                               aCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGc                                      >  3:4335/1‑116 (MQ=255)
                                                                                                                                               aCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGc                                      <  4:4335/116‑1 (MQ=255)
                                                                                                                                                ccGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAatta    <  4:18352/149‑1 (MQ=255)
                                                                                                                                                  ggATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAattatt  <  7:145355/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATCAATAACTTATTCTTAAGTATTTGACAGCACTGAATGTCAAAACAAAACCTTCACTCGCAACTAGAATAACTCCCGCTATCATCATTAACTTTATTTATTACCGTCATTCATTTCTGAATGTCTGTTTACCCCTATTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAATCTTTGTTTATTTGCAATTATT  >  NC_000913/2313006‑2313300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: