Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,339,310 G→A 7.0% V37V (GTC→GTT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,339,3100GA 7.0% 172.3 / 2.7 57V37V (GTC→GTT) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base G (30/23);  new base A (2/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

ATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGATTCAAA  >  NC_000913/2339163‑2339456
                                                                                                                                                   |                                                                                                                                                  
aTAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGa                                                                                                                                                   >  1:212485/1‑149 (MQ=255)
   gACCGCCGAGGCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGATCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACAt                                                                                                                                                <  3:152487/149‑1 (MQ=255)
    aCCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATc                                                                                                                                               <  2:100372/149‑1 (MQ=255)
      cgccgAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTg                                                                                                                                             >  4:224202/1‑149 (MQ=255)
      cgccgAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTg                                                                                                                                             >  7:114614/1‑149 (MQ=255)
      cgccgAGTCACCATGGGGATGGTATTTACCGATTACCTCACAAACGACACGGGCAGAGTTTTTATAGCCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGCCTTCAGGCCATCTCGGACATCTg                                                                                                                                             >  7:87213/1‑149 (MQ=255)
      cgccgAGTCACCATGGGGATGGAATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCATTGTAGGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTAAGGCCATCACGGACATCTg                                                                                                                                             >  8:107556/1‑149 (MQ=255)
       gccgAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTgg                                                                                                                                            <  4:104559/149‑1 (MQ=255)
               aCCATGGGGATGGTATTTACCGATTACGTCCCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCAc                                                                                                                                    >  1:58887/1‑149 (MQ=255)
               aCCATGGGGATGGTATTTACCGATTACGTCACCCACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCAc                                                                                                                                    >  3:64926/1‑149 (MQ=255)
                ccATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACg                                                                                                                                   >  2:113067/1‑149 (MQ=255)
                       gATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCcaac                                                                                                                             >  5:226213/1‑148 (MQ=255)
                         tGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAAt                                                                                                                          <  5:86702/149‑1 (MQ=255)
                              tttACCGATTACGTCCCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATTGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGAcc                                                                                                                      <  2:58887/148‑1 (MQ=255)
                                 aCCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACa                                                                                                                  >  5:209986/1‑149 (MQ=255)
                                      ttACGGCACCTACGACAAGGGCATATTTTTTATAGGCTTTGTGCCAGTCATTGCCTAGTACGTTCATGGTGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACACTGACCGACATCGCa                                                                                                             <  4:37898/149‑1 (MQ=255)
                                             aCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCa                                                                                                      <  6:226213/149‑1 (MQ=255)
                                               cAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGa                                                                                                    <  2:212485/149‑1 (MQ=255)
                                                aaCGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGTCGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGAt                                                                                                   <  7:107556/149‑1 (MQ=255)
                                                  cGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATGTCGGTCATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAg                                                                                                 <  2:195678/149‑1 (MQ=255)
                                                  cGACACGGGCAGATTTTTTATAGGATTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCTGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAg                                                                                                 <  7:242656/149‑1 (MQ=255)
                                                          gCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGAACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctctt                                                                                         >  3:229674/1‑149 (MQ=255)
                                                          gCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGAACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctctt                                                                                         >  3:226257/1‑149 (MQ=255)
                                                              aTTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagc                                                                                     >  8:149509/1‑149 (MQ=255)
                                                              aTTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagc                                                                                     >  8:149511/1‑149 (MQ=255)
                                                               ttttttATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACAGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCACCAATGACCGACATCGCATAATCCAGATAGGagctcttcagct                                                                                    >  7:221152/1‑149 (MQ=255)
                                                                    tctaGGCGTTGTGCCAGTCATTGCCTAGTACGTGCATGGCGTAAAGTACGCGACGGTGTACCGGCTGCAGGCCAGCTCGGACATCTGGCAGCGCACTGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctcttc                                                                                <  2:209003/146‑1 (MQ=255)
                                                                        ggCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGCCCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCaa                                                                           >  5:236060/1‑149 (MQ=255)
                                                                         gCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGAACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAAt                                                                          <  4:226257/149‑1 (MQ=255)
                                                                         gCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGAACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAAt                                                                          <  4:229674/149‑1 (MQ=255)
                                                                              gTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATATGAGCTCTTCAGCTCTTCCTCAAtgatga                                                                     >  8:89686/1‑149 (MQ=255)
                                                                               ttCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAc                                                                    <  1:113067/149‑1 (MQ=255)
                                                                                 ccAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATTTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCg                                                                  >  4:135162/1‑149 (MQ=255)
                                                                                   aGTCATTGCCTAGTACGTTCCTGGCGTAAAGTACGCGACGGGGTACCGGCTTCAGGCCATCGCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAAt                                                                          <  1:205677/139‑1 (MQ=255)
                                                                                   aGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGgt                                                                <  7:149509/149‑1 (MQ=255)
                                                                                   aGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGgt                                                                <  7:149511/149‑1 (MQ=255)
                                                                                   aGTCATTGCCTAGTACGTTCATGGCGTAAAGGACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCTCACTGCCAACAATGAACGACATAGCATAATACAGATAGGAGCTCGTCAGCTCTTCATCAAt                                                                          >  2:205677/1‑139 (MQ=255)
                                                                                       aTTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTaa                                                            >  3:30382/1‑149 (MQ=255)
                                                                                         tGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCa                                                                            >  6:213430/1‑131 (MQ=255)
                                                                                           ccTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtc                                                        <  7:169730/149‑1 (MQ=255)
                                                                                             tAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctc                                                      >  8:187514/1‑149 (MQ=255)
                                                                                                tACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCg                                                   >  6:47301/1‑149 (MQ=255)
                                                                                                       aTGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGt                                             <  6:209986/148‑1 (MQ=255)
                                                                                                           cGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCt                                         >  1:64503/1‑148 (MQ=255)
                                                                                                                  tACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAAc                                 >  6:36715/1‑149 (MQ=255)
                                                                                                                   aCGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAAcc                                >  1:169093/1‑149 (MQ=255)
                                                                                                                    cgcgACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAAcc                                <  2:169093/148‑1 (MQ=255)
                                                                                                                     gcgACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCAAAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGc                              >  7:9518/1‑149 (MQ=255)
                                                                                                                       gACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTa                            >  5:86428/1‑149 (MQ=255)
                                                                                                                        aCGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTAt                           <  2:64503/149‑1 (MQ=255)
                                                                                                                               aCCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTa                    >  5:216585/1‑149 (MQ=255)
                                                                                                                                ccGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTAc                   >  1:144022/1‑149 (MQ=255)
                                                                                                                                     ttCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTAt              >  7:44148/1‑149 (MQ=255)
                                                                                                                                         ggCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCg          >  5:35778/1‑149 (MQ=255)
                                                                                                                                           ccATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGa        <  3:194414/149‑1 (MQ=255)
                                                                                                                                            cATCTCGGACATCTGGCAGCGCACGGCCAACAAAGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGGCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGAt       <  4:64926/149‑1 (MQ=255)
                                                                                                                                             aTCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGAtt      <  5:36715/149‑1 (MQ=255)
                                                                                                                                                  ggACATCTGTCAGCGCACGGCCAACAATGAGCGACATCGCATTATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGATTCaaa  <  7:89686/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCTCGCAAGGTCGCTCATCTAACCGCTATCCCTCTACTGTATCCCGGATTCAAA  >  NC_000913/2339163‑2339456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑